Effect of polyvinyl alcohol on survival and function of angora buck spermatozoa following cryopreservation

Tekin, Koray; Daşkın, Ali

doi:10.1016/j.cryobiol.2019.05.004

Cited by 21 publications

(11 citation statements)

References 34 publications

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“…However, AFPs are not easy to extract from natural resources such as fungi, bacteria, plants, insects and fish that are adapted to cold environments [87] and AFPs derived from other organisms can be detrimental to sperms or inseminated female animals. Tekin and Daşkın [88] utilized polyvinyl alcohol (PVA) instead of AFPs for ice recrystallization inhibition and showed that supplementation enhances motility, viability, acrosome integrity and mitochondrial activity in frozen-thawed rabbit sperms. By stabilization of cell membrane, supplementation of a silk protein, sericin [89] and cholesterol-loaded cyclodextrin [90] improved motility and quality of frozen-thawed rabbit sperm.…”

Section: Effects Of Supplementsmentioning

confidence: 99%

Strategies for Highly Efficient Rabbit Sperm Cryopreservation

Nishijima

Kitajima

Matsuhisa

et al. 2021

Animals

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The rabbit is a valuable animal for both the economy and biomedical sciences. Sperm cryopreservation is one of the most efficient ways to preserve rabbit strains because it is easy to collect ejaculate repeatedly from a single male and inseminate artificially into multiple females. During the cooling, freezing and thawing process of sperms, the plasma membrane, cytoplasm and genome structures could be damaged by osmotic stress, cold shock, intracellular ice crystal formation, and excessive production of reactive oxygen species. In this review, we will discuss the progress made during the past years regarding efforts to minimize the cell damage in rabbit sperms, including freezing extender, cryoprotectants, supplements, and procedures.

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Section: Effects Of Supplementsmentioning

confidence: 99%

Strategies for Highly Efficient Rabbit Sperm Cryopreservation

Nishijima

Kitajima

Matsuhisa

et al. 2021

Animals

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“…Pattern formation during ice growth has been widely studied in the last decades among a great variety of both scientific and engineering communities due to its significance in both condensed matter physics, , atmospheric science, icephobic materials design, , material processing engineering, − and cryobiology. − Although there are plenty of advancements in the last centuries, one interesting but puzzling growth habit of ice is the occurrence of tilted growth of ice cell/dendrite in undercooled pure water and aqueous solutions − distinct from a planar dendrite in its basal plane at small undercooling. In both free growth and unidirectional growth experiments, lamellar ice in the form of either an array of dendrites or a single ice dendrite was usually reported to grow in a “noncrystallographic” or, in other words, a tilted direction deviating from the basal plane.…”

Section: Introductionmentioning

confidence: 99%

Tilting Behavior of Lamellar Ice Tip during Unidirectional Freezing of Aqueous Solutions

Zhang

Wang

et al. 2021

Langmuir

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Freezing of ice has been largely reported from many aspects, especially its complex pattern formation. Ice grown from liquid phase is usually characteristic of lamellar morphology which plays a significant role in various domains. However, tilted growth of ice via transition from coplanar to non-coplanar growth in directional solidification has been paid little attention in previous studies and there is misleading explanation of the formation of tilted lamellar ice. Here, we in-situ investigated the variations of tilting behavior of lamellar ice tip under different conditions within a single ice crystal with manipulated orientation via unidirectional freezing of aqueous solutions. It is found that tilted growth of ice tips is sensitive to pulling velocity and solute type. These experimental results reveal intrinsic tilted growth behavior of lamellar ice and enrich our understanding in pattern formation of ice.

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“…Taking the results of the assessment of sperm plasma membranes (Figure 2c) into consideration, we speculate that treatment with cBiMPS is accompanied by overstimulation of the cAMP-dependent signaling cascades leading to membrane destabilization in the presence of BSA, and that the addition of PVA may weaken the deleterious influences of cBiMPS on membrane stabilization. Moreover, it is expected that PVA can act as a protector of sperm plasma membranes, considering that PVA is effective for maintaining the motility and acrosome in cryopreserved spermatozoa (Nabeel et al, 2019;Tekin & Das ¸kın, 2019).…”

Section: Resultsmentioning

confidence: 99%

Polyvinyl alcohol, but not bovine serum albumin, promotes the induction of full‐type hyperactivation in boar cyclic AMP analog‐treated spermatozoa

Sai

Harayama

2022

Animal Science Journal

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This study aimed to verify the effects of polyvinyl alcohol (PVA) and bovine serum albumin (BSA) on the induction of full-type hyperactivation in boar spermatozoa treated with a cyclic AMP analog (cBiMPS). Washed spermatozoa were treated with cBiMPS (100 μM) for 180 min. As shown in the assessment of sperm motility, PVA (0.05%-0.4%) significantly promoted the induction of full-type hyperactivation, whereas BSA (0.025%-0.4%) did not affect the induction. In comparative experiments, BSA (0.4%) effectively promoted the induction of full-type hyperactivation in bovine spermatozoa treated with cBiMPS, calyculin A (a protein phosphatase inhibitor), and digoxin (a Na + /K + -ATPase inhibitor), while PVA (0.1%) did not affect the induction.Western blotting showed that protein tyrosine phosphorylation states of >50 kDa sperm proteins were effectively enhanced by treatment with cBiMPS in the PVA/BSAfree medium and not affected by the addition of PVA (0.1%). The assessment of plasma membrane integrity indicated that BSA (0.4%) significantly decreased spermatozoa with intact plasma membranes. These results indicate that PVA (0.1%) promotes the induction of full-type hyperactivation and does not influence the protein tyrosine phosphorylation states in boar cBiMPS-treated spermatozoa. They also suggest that BSA should not be added to medium containing cBiMPS for boar spermatozoa.

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Effect of polyvinyl alcohol on survival and function of angora buck spermatozoa following cryopreservation

Cited by 21 publications

References 34 publications

Strategies for Highly Efficient Rabbit Sperm Cryopreservation

Strategies for Highly Efficient Rabbit Sperm Cryopreservation

Tilting Behavior of Lamellar Ice Tip during Unidirectional Freezing of Aqueous Solutions

Polyvinyl alcohol, but not bovine serum albumin, promotes the induction of full‐type hyperactivation in boar cyclic AMP analog‐treated spermatozoa

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