With the explosive growth of energy storage devices, commonly used lithium-ion batteries can hardly match the increased demands of energy-density, lithium-sulfur batteries have been regarded as promising candidates due to their high energy-density(2600 Wh/Kg) and low cost. However, lithium sulfur batteries still face many problems such as polysulfide shuttle effect originated from dissolution of lithium polysulfide, that leaded to the capacity fading of batteries and corrosion of lithium anodes, greatly limited their practical applications. To settle these problems, many remarkable developments have been made by the construction of separators with the dimension design of employing the materials related by metal-organic frameworks(MOFs), shedding a light on the improvement of lithium-sulfur batteries. Herein, this article aims to summarize recent developments in MOF-related separators of lithium-sulfur batteries in terms of the structural dimensions of MOF-related materials, including their architectural compositions in separators and facilitation mechanisms of battery performances. At last, an outlook section was provided to discuss remaining challenges of separators as well as potential research directions and possible prospects, to offer new thoughts for future studies of lithium-sulfur batteries.