Effect of pomegranate peel polyphenols on human prostate cancer PC-3 cells in vivo

Ma, Guizhi; Wang, Chunmei; Li, Li; Ding, Nan; Gao, Xiaoli

doi:10.1007/s10068-015-0247-0

Cited by 31 publications

(18 citation statements)

References 13 publications

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“…Pomegranate extracts have broad bioactivities including antimicrobial, antiinflammatory and anti‐tumor effects (Betanzos‐Cabrera et al, ; Ma et al, ; Park et al, ) and have been used in traditional Uyghur medicine for thousands of years. Our results show that GA decreased membrane CYP51 enzyme activity and increased fungal lanosterol in a dose‐dependent manner.…”

Section: Discussionmentioning

confidence: 99%

Antifungal Activity of Gallic Acid In Vitro and In Vivo

Dawuti³

et al. 2017

Phytotherapy Research

148

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Gallic acid (GA) is a polyphenol natural compound found in many medicinal plant species, including pomegranate rind (Punica granatum L.), and has been shown to have antiinflammatory and antibacterial properties. Pomegranate rind is used to treat bacterial and fungal pathogens in Uyghur and other systems of traditional medicine, but, surprisingly, the effects of GA on antifungal activity have not yet been reported. In this study, we aimed to investigate the inhibitory effects of GA on fungal strains both in vitro and in vivo. The minimal inhibitory concentration (MIC) was determined by the NCCLS (M38-A and M27-A2) standard method in vitro, and GA was found to have a broad spectrum of antifungal activity, with MICs for all the tested dermatophyte strains between 43.75 and 83.33 μg/mL. Gallic acid was also active against three Candida strains, with MICs between 12.5 and 100.0 μg/mL. The most sensitive Candida species was Candida albicans (MIC = 12.5 μg/mL), and the most sensitive filamentous species was Trichophyton rubrum (MIC = 43.75 μg/mL), which was comparable in potency to the control, fluconazole. The mechanism of action was investigated for inhibition of ergosterol biosynthesis using an HPLC-based assay and an enzyme linked immunosorbent assay. Gallic acid reduced the activity of sterol 14α-demethylase P450 (CYP51) and squalene epoxidase in the T. rubrum membrane, respectively. In vivo model demonstrated that intraperitoneal injection administration of GA (80 mg/kg d) significantly enhanced the cure rate in a mice infection model of systemic fungal infection. Overall, our results confirm the antifungal effects of GA and suggest a mechanism of action, suggesting that GA has the potential to be developed further as a natural antifungal agent for clinical use. Copyright © 2017 John Wiley & Sons, Ltd.

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Section: Discussionmentioning

confidence: 99%

Antifungal Activity of Gallic Acid In Vitro and In Vivo

Dawuti³

et al. 2017

Phytotherapy Research

148

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“…They play an important role in protection of plants from microbial and insect attack. Many studies reported that flavonoids exhibit various effects as antioxidant (Siahpoosh et al, 2016;Balci & Özdemir, 2016), anti-cancer (Androutsopoulos et al, 2010;Ma et al, 2015), anti-allergic (Park et al, 2006), anti-thrombotic and vasodilatory (Rahimi et al, 2010), anticholinesterase (Ertas et al, 2016) actions. Finally, because of their UV-absorbing properties, flavonoids protect plants from the UV radiation of the sun and scavenge UV-generated reactive oxygen species (Shirley, 1996).…”

Section: Introductionmentioning

confidence: 99%

Simultaneous quantification of phenolic acids and flavonoids in Chamaerops humilis L. using LC–ESI-MS/MS

et al. 2018

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On this study, heated reflux extraction method has been used to identify the phenolic compounds from C. humilis var. argentea leaflets, rachis and fruits. Extractions were performed in both ultrapure water and 80% methanol solvents. The efficiency of procedures was determined in terms of the quality and quantity of phenolic acids and flavonoids identified. Chamaerops extracts have been characterized by high concentrations of phenolic compounds, which play a crucial role in protection against various diseases. LC-MS/MS was used to determine the chemical profile of various extracts obtained from Chamaerops. The results showed that the major components in leaflets and fruits extracts were quinic, malic and chlorogenic acids. On addition, nine minor acidic components were identified. Dn the other hand, rutin and hesperidin were found to be the major flavonoids. The methanol extract was shown as being the most efficient to identify phenolic compounds in C. humilis. Keywords: Chamaerops humilis L.; LC-ESO-MS/MS; phenolic compounds; chemical composition; extracts.Practical Application: Chamaerops humilis L. rich in flavonoids and phenolic acids was a great promising source of different bioactive components.

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“…Extracts isolated from the fruit peel have been explored in vivo for their preventive role against a wide array of communicable and noncommunicable diseases. Similarly, extracts derived from the fruit peel fraction have been shown to express ameliorative effect against microbial pathogenesis, and certain chronic ailments including those of cardiovascular diseases (Neyrinck et al 2013), inflammatory disorders (Bachoual et al 2011), various types of cancer (Lansky and Newman 2007;Ma et al 2015) and diabetes (Karaaslan et al 2014).…”

Section: Introductionmentioning

confidence: 99%

Oxidative and Microbial Stability of Pomegranate Peel Extracts and Bagasse Supplemented Cookies

Ismail

Akhtar

Riaz

et al. 2016

Journal of Food Quality

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This study aimed at exploring pomegranate peel (PoP) for its potential as an ingredient of choice in baked products primarily to stabilize lipids and inhibit microbiological spoilage. Pomegranate peel extracts (PoPx) and pomegranate peel bagasse (Pbg) were supplemented to wheat flour cookies at 0.25–1.0% and 1.5–7.5%, respectively. Cookies supplemented with 7.5% Pbg reduced caloric contents and significantly (P < 0.05) enhanced dietary fiber and inorganic residues of the product. Positive correlation was observed for cookies phenolics contents with 2,2‐diphenyl‐1‐picrylhydrazyl (DPPH) and ferric reducing antioxidant power assay. Total phenolics recovery from Pbg and PoPx was shown to range from 78.35 to 315.7 mg GAE/100g), respectively. PoPx supplemented cookies, holding significant antioxidant properties, inhibited thiobarbituric acid number by 67% and reduced growth of aerobic counts (2.04–1.30 log10 cfu/g) and yeasts/molds (1.70–1.05 log10 cfu/g). Sensory evaluation of Pbg supplemented cookies indicated relatively lower score for texture and crispiness while other treatment combinations were ranked acceptable at 9‐point hedonic scale for all sensory parameters. Practical Applications Synthetic antioxidants and antimicrobials being injudiciously exploited as food preservatives are hampering quality of food and life. Programmed replacement of preservative cum intoxicating agents with relatively least to nontoxic natural phytochemicals is referred to be the sustainable and safe approach in food preservation. On account of its tremendous food quality enhancement features, PoP vide its ethnobotanical extracts deliver significant antioxidant, antimicrobial and nutraceuticals properties. As witnessed in current study, PoPx supplementation in bakery products has a prospective role in inhibiting lipid oxidation and microbiological spoilage and, hence can be considered as viable substitute of synthetic preservatives. Nutritional enhancement features of peel bagasse further endeavors underutilized fruit waste as ingredient of choice in formulating virtually balanced therapeutic formulation against certain critical ailments.

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Effect of pomegranate peel polyphenols on human prostate cancer PC-3 cells in vivo

Cited by 31 publications

References 13 publications

Antifungal Activity of Gallic Acid In Vitro and In Vivo

Antifungal Activity of Gallic Acid In Vitro and In Vivo

Simultaneous quantification of phenolic acids and flavonoids in Chamaerops humilis L. using LC–ESI-MS/MS

Oxidative and Microbial Stability of Pomegranate Peel Extracts and Bagasse Supplemented Cookies

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