Effect of pore diffusional resistance on biocatalytic activity of Burkholderia cepacia lipase immobilized on SBA-15 hosts

Jaladi, Hemachand; Katiyar, Amit; Thiel, Stephen W.; Guliants, Vadim V.; Pinto, Neville G.

doi:10.1016/j.ces.2008.10.042

Cited by 38 publications

(31 citation statements)

References 24 publications

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“…The effect of diffusion is limited by the capacity of the substrate to diffuse in the pores used to achieve immobilization of the enzyme on mesoporous supports. 47,48 In this study, the pore volume of the control silica was lower than that of the modified silica; the same was not true for BCL on control silica or BCL on modified silica, since the pore volume for BCL on control silica was higher than the PIL modified silica, possibly due to the formation of a lipase multilayer on the surface of the control silica. Differently from the IB on modified silica, for the lipase bound to the support, both on the surface and in the pores of the silica, the increase in the pore diameter of the support was caused by the presence of PIL (3.02 nm for control silica and 4.17 nm for modified silica).…”

Section: Mass Transfer Coefficientmentioning

confidence: 58%

Analysis of the Performance of a Packed Bed Reactor to Production Ethyl Esters From Crude Vegetable Oil Using Lipase Immobilized in Silica Modified With Protic Ionic Liquid

Santana¹,

Oliveira²,

Carvalho³

et al. 2018

Quim. Nova

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The objective this study was to evaluated the influence of silica modified with protic ionic liquid (PIL) on an immobilized biocatalyst by covalent binding of Burkholderia cepacia lipase (BCL) in a PBR and in the transesterification reaction, by characterization and mass transfer of the system studied. The results showed that maximum conversion of ethyl esters was 39 ± 1.73% at 96 h (IB on control silica) and 47 ± 2.0% in 72 h (IB on modified silica). The PIL also influenced the analysis of residence time distribution (RTD) with the mean time ranging from 14.94 (control silica) to 25 min (modified silica). Variation of the reaction parameters (temperature, flow rate and molar ratio) positively influenced the mass transfer coefficient (kc) which varied from 0.95 × 10 −4 to 2.39 × 10 −4 m s −1 (IB on control silica) and from 1.22 × 10 −4 to 3.06 × 10 −4 m s −1 (IB on modified silica).

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Section: Mass Transfer Coefficientmentioning

confidence: 58%

Analysis of the Performance of a Packed Bed Reactor to Production Ethyl Esters From Crude Vegetable Oil Using Lipase Immobilized in Silica Modified With Protic Ionic Liquid

Santana¹,

Oliveira²,

Carvalho³

et al. 2018

Quim. Nova

View full text Add to dashboard Cite

show abstract

“…In the light of this result we hypothesize that the protein concentration in the large Zera-Xyl oligomers impairs the conformational fluctuations, namely “breathing” [45], which would be needed to accommodate the substrate in the optimum environment. This result was not unexpected because in insoluble enzymes the diffusion rates of substrates through the aggregate may be limited [46]. We are currently engineering more swollen Zera-Xyl polymers to improve enzyme efficiency.…”

Section: Discussionmentioning

confidence: 92%

The Expression of a Xylanase Targeted to ER-Protein Bodies Provides a Simple Strategy to Produce Active Insoluble Enzyme Polymers in Tobacco Plants

et al. 2011

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BackgroundXylanases deserve particular attention due to their potential application in the feed, pulp bleaching and paper industries. We have developed here an efficient system for the production of an active xylanase in tobacco plants fused to a proline-rich domain (Zera) of the maize storage protein γ-zein. Zera is a self-assembling domain able to form protein aggregates in vivo packed in newly formed endoplasmic reticulum-derived organelles known as protein bodies (PBs).Methodology/Principal FindingsTobacco leaves were transiently transformed with a binary vector containing the Zera-xylanase coding region, which was optimized for plant expression, under the control of the 35S CaMV promoter. The fusion protein was efficiently expressed and stored in dense PBs, resulting in yields of up to 9% of total protein. Zera-xylanase was post-translationally modified with high-mannose-type glycans. Xylanase fused to Zera was biologically active not only when solubilized from PBs but also in its insoluble form. The resistance of insoluble Zera-xylanase to trypsin digestion demonstrated that the correct folding of xylanase in PBs was not impaired by Zera oligomerization. The activity of insoluble Zera-xylanase was enhanced when substrate accessibility was facilitated by physical treatments such as ultrasound. Moreover, we found that the thermostability of the enzyme was improved when Zera was fused to the C-terminus of xylanase.Conclusion/SignificanceIn the present work we have successfully produced an active insoluble aggregate of xylanase fused to Zera in plants. Zera-xylanase chimeric protein accumulates within ER-derived protein bodies as active aggregates that can easily be recovered by a simple density-based downstream process. The production of insoluble active Zera-xylanase protein in tobacco outlines the potential of Zera as a fusion partner for producing enzymes of biotechnological relevance. Zera-PBs could thus become efficient and low-cost bioreactors for industrial purposes.

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“…Table 3 reports the data obtained from the nitrogen adsorption-desorption isotherms of the functionalized absorbents (particle size: 90-212 µm). to enhance the mass-transfer rate of solution phase species, i.e., toxic heavy metal cations to the pore surfaces where the functional groups are located [30].…”

Section: Elemental Analysismentioning

confidence: 99%

Removal of Mercury from Simulated Flue Gas Desulfurization Wastewater using Functionalized Silica

Guliants¹,

Sanghavi²,

J³

et al. 2017

Preprint

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We report highly promising results for Hg2+ removal from FGD wastewater to address new EPA effluent limitation guidelines (ELG). Their novelty stems from the use of realistic Hg concentrations in the presence of total dissolved solids typical for FGD wastewater. Although similar adsorbents were reported previously, they were studied at unrealistically high Hg concentrations in deionized water. We demonstrated that MPTMS-silica and MBT-silica can reduce Hg2+ below its maximum contaminant levels (MCLs) and ELGs independent of the concentration of major ions.

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Effect of pore diffusional resistance on biocatalytic activity of Burkholderia cepacia lipase immobilized on SBA-15 hosts

Cited by 38 publications

References 24 publications

Analysis of the Performance of a Packed Bed Reactor to Production Ethyl Esters From Crude Vegetable Oil Using Lipase Immobilized in Silica Modified With Protic Ionic Liquid

Analysis of the Performance of a Packed Bed Reactor to Production Ethyl Esters From Crude Vegetable Oil Using Lipase Immobilized in Silica Modified With Protic Ionic Liquid

The Expression of a Xylanase Targeted to ER-Protein Bodies Provides a Simple Strategy to Produce Active Insoluble Enzyme Polymers in Tobacco Plants

Removal of Mercury from Simulated Flue Gas Desulfurization Wastewater using Functionalized Silica

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