IntroductionPost-activation potentiation (PAP) can be defined as the acute enhancement in muscle performance after preload stimulation that occurs during strength exercises or warm-up protocols, and the contractile history of any muscle group can directly influence the presented performance. The purpose of this study was to compare the results of motoric performance tests carried out at two different times of the day using three different PAP protocols.MethodsThirty-two male basketball players with at least 2 years of training experience and who competed at the national level were recruited for this study [age: 21.80 ± 1.91 years; body height: 178.40 ± 6.85 cm; body mass: 72.50 ± 7.16 kg; body mass index (BMI): 22.81 ± 2.28]. After control and experimental-specific warm-up (SWU) sessions, participants completed a countermovement jump (CMJ) and seated medicine ball throw (SMBT). The SWU protocol was developed based on the athlete’s typical warm-up routine. The experimental warm-ups included the same exercises as the SWU, with one set of bench presses for five repetitions at 80% (80% PAP) and one repetition at 100% of 1 RM (100% PAP). Each of the protocols consists of 15 min. The SWU and experimental warm-up sessions were completed in a random, counterbalanced order, completed in a period of 3-weeks.ResultsAccording to the research findings, carrying out the protocols in the evening led to a larger improvement in SMBT than in the morning (F = 35.94, p < 0.001, η2p = 0.537). Additionally, the SMBT results were statistically more significant in the group that received 80% PAP compared to the SWU (p = 0.034), and the SMBT results were also higher in the group that received 100% PAP compared to the SWU (p = 0.002). Furthermore, the interaction effect (time × PAP) was statistically significant in SMBT (F = 6.39, p = 0.003, η2p = 0.17).DiscussionThese results may provide more particular recommendations than previously thought to basketball coaches for the planning of basketball-specific PAP exercises prior to the start of training programs.