Effect of potassium chloride and calcium chloride induced stress on in vitro cultures of Bacopa monnieri (L.) Pennell and accumulation of medicinally important bacoside A

Ahire, Mahendra L.; Laxmi, Sri; Walunj, P. R.; Kishor, P. B. Kavi

doi:10.1007/s13562-013-0220-z

Cited by 31 publications

(9 citation statements)

References 73 publications

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“…In the present study enhanced accumulation of bacoside A content (Fig. 9) was noticed under the salt stress which corroborates with the earlier findings of Ahire et al (2013Ahire et al ( , 2014.…”

Section: Discussionsupporting

confidence: 93%

The vacuolar proton pyrophosphatase gene (SbVPPase) from the Sorghum bicolor confers salt tolerance in transgenic Brahmi [Bacopa monnieri (L.) Pennell]

Ahire

Kumar

Punita

et al. 2018

Physiol Mol Biol Plants

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Plants overcome the effect of Na toxicity either by excluding Na at the plasma membrane or by sequestering them into the vacuoles. Influx of Na ions into the plant vacuoles is usually driven by H generated by vacuolar-type H-ATPase as well as vacuolar proton pyrophosphatse (VPPase). In the present study, we have developed transgenics via containing the recombinant vector pCAMBIA2300- gene. Transformants were produced using nodal explants. Transformants were confirmed by PCR and DNA blot analysis. qPCR analysis showed higher transcript levels of compared to untransformed control (UC). Higher VPPase activity was recorded in transgenics compared to UC. Under 150 mM salt stress, transgenic shoots showed enhanced Na accumulation with better biomass production, increased glycine betaine content, and total soluble sugar levels than UC. Transgenic shoots showed 2.9-3.8-folds lower levels of malondialdehyde content indicating lesser membrane damage. Increase in antioxidant enzyme activities (1.4-3.2-folds) was observed in transgenics compared to UC. Transgenics also displayed 7.3-9.0-folds enhanced accumulation of the medicinally important compound bacoside A. Increased biomass production, accumulation of Na, osmolytes (glycine betaine, sugars etc.), and elevated antioxidant enzyme activities indicate better osmotic adjustment in transgenics by compartmentalization of Na into the vacuoles under salt stress conditions. Thus, overexpression of in alleviated salt stress by sequestering Na.

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Section: Discussionsupporting

confidence: 93%

The vacuolar proton pyrophosphatase gene (SbVPPase) from the Sorghum bicolor confers salt tolerance in transgenic Brahmi [Bacopa monnieri (L.) Pennell]

Ahire

Kumar

Punita

et al. 2018

Physiol Mol Biol Plants

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“…We observed growth of approximately 10 mm when exposed to KI concentrations up to 70.08 µM, which was 60 mm less than the control group (Fig 5B). Potassium affects plant growth and cell metabolism , and at toxic levels it will inhibit the development of the aerial portion (Ahire et al, 2014). The root length data fitted a linear model, and decreased by approximately 30 mm at increasing K concentrations, from 0 to 100 µM KI (Fig 5C).…”

Section: Test 3: Ki Effects On the Growth Of Purple Basilmentioning

confidence: 90%

“…Above this KI concentration, plants developed normally (Fig 5A). Ahire et al (2014) reported that salt stress in plants will affect plant development and growth. Surplus K appeared to cause stress that affected not only the number of shoots but also the growth of the aerial portion.…”

Section: Test 3: Ki Effects On the Growth Of Purple Basilmentioning

confidence: 99%

“…0.2g) was observed at 70.54 µM KI (Fig 5D). Increased levels of K + may cause several problems for plants, including competition for nutrients (Shabala and Cuin, 2007;Andriolo et al, 2010) and damage to cell plasma membranes due to homeostatic imbalances -resulting in decreased plant growth (Ahire et al, 2014).…”

Section: Test 3: Ki Effects On the Growth Of Purple Basilmentioning

confidence: 99%

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In vitro cultivation of purple basil Ocimum basilicum L. ‘red rubin’ at different levels of salts, charcoal, sucrose and potassium iodine

Silva¹,

Nascimento²,

Barbosa³

et al. 2017

Aust J Crop Sci

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Ocimum basilicum'red rubin' is highly valued for its essential oil, and is widely used in folk medicine, but few studies examining in vitro propagation techniques for this basil variety are available. We examined the development of red rubin basil seedlings grown in vitro in different concentrations of mineral salts, charcoal combined with sucrose, and potassium iodine in three series of tests. In the first series, five concentrations (0, 25, 50, 75 and 100%) of Murashige and Skoog salts (MS culture medium) were used to germinate sterilized seeds of O. basilicum. In the second test, to evaluate the combined effects of charcoal and sucrose on basil growth, three concentrations of charcoal (0, 3.0, and 4.5 g/L) and two of sucrose (30 and 60 g/L) were used. In the third test, the seeds were sown onto culture medium enriched with five different concentrations of potassium iodine (0, 25, 50, 75 and 100µM); 0.5 μM benzyl aminopurine (BAP) and 1.0 μM naphthalene acetic acid (NAA) were added to all germination media. A completely randomized experimental design was used in all tests, with five replicates, four vials per test, and 4 seeds per vial. In Test 2, seedling oxidation (%) and the numbers of leaves and shoots were submitted to non-parametric analysis using the Kruskal Wallis test (p ≤0.05). The other data were submitted to analysis of variance (p≤0.05). The qualitative means were evaluated by the Tukey test (p≤0.05), and the quantitative means by polynomial regression (p≤0.05). The results showed that 100% MS culture medium was most indicated for 'red rubin' seed germination and aerial portion production, while 70-80% MS was indicated for root production. A sucrose level of 30 g/L (standard) combined with 4.5 g/L of activated charcoal allowed the best seedlings development and inhibited callus formation. Activated charcoal limited copper and zinc uptake by O. basilicum seedlings, but not enough to hamper their development. Increasing concentrations of potassium iodine reduced all of the growth variables analyzed in this basil variety.Keywords: Ocimum basilicum L., micropropagation, medicinal plant, culture medium. Abbreviation: AC_ activated charcoal, APL_ aerial portion length, BAP_ benzylaminopurine, Cu_ Copper; CSD1 and CSD2_ gene families in Arabidopsis, DMAP_ dry mass of the aerial portion, DMRC_ dry mass of the root or of the root + callus portion, FMAP_ fresh masses of the aerial portions, FMRC_ fresh masses of the root, KI_ potassium iodide, miRNAs_ micro RNAs, MS_ Murashige and Skoog, NAA_ naphthalene acetic acid, NC-presence of callus, NL_ number of leaves, NS_ numbers of shoots, OX_ oxidized plants, PVP_polyvinyl pyrrolidone, RL_ root, SISVAR_ statistical analysis system, SOD_ superoxide dismutase, Zn_ Zinc.

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“…The salt stress decreased the anthocyanin level in the salt-sensitive species [46]. The effect of KCl and CaCl 2 induced stress on in vitro cultures of Bacopa monnieri enhanced the accumulation of medicinally important bacoside A content [47]. An improved synthesis of vinblastine and vincristine was observed in C. roseus embryogenic tissue culture by using sodium chloride (NaCl) as an elicitor [48].…”

Section: Lightmentioning

confidence: 99%

Impact of Abiotic Elicitors on In vitro Production of Plant Secondary Metabolites: A Review

Naik¹,

Al–Khayri²

2016

JARB

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Effect of potassium chloride and calcium chloride induced stress on in vitro cultures of Bacopa monnieri (L.) Pennell and accumulation of medicinally important bacoside A

Cited by 31 publications

References 73 publications

The vacuolar proton pyrophosphatase gene (SbVPPase) from the Sorghum bicolor confers salt tolerance in transgenic Brahmi [Bacopa monnieri (L.) Pennell]

The vacuolar proton pyrophosphatase gene (SbVPPase) from the Sorghum bicolor confers salt tolerance in transgenic Brahmi [Bacopa monnieri (L.) Pennell]

In vitro cultivation of purple basil Ocimum basilicum L. ‘red rubin’ at different levels of salts, charcoal, sucrose and potassium iodine

Impact of Abiotic Elicitors on In vitro Production of Plant Secondary Metabolites: A Review

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