Effect of potassium deficiency on the plasma membrane H<sup>+</sup>‐ATPase of the wood ray parenchyma in poplar

Arend, Matthias; Monshausen, Gabriele B.; Wind, Christa; Weisenseel, Manfred H.; Fromm, Jörg

doi:10.1111/j.1365-3040.2004.01236.x

Cited by 26 publications

(5 citation statements)

References 37 publications

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“…Extracellular pH and H ϩ fluxes at the surface of Arabidopsis root hairs were measured with H ϩ -selective microelectrodes. The microelectrode and reference electrode were fabricated and calibrated as described (47), see also SI Materials and Methods for detailed protocols.…”

Section: Methodsmentioning

confidence: 99%

Oscillations in extracellular pH and reactive oxygen species modulate tip growth of Arabidopsis root hairs

Monshausen

Bibikova

Messerli

et al. 2007

Proc. Natl. Acad. Sci. U.S.A.

376

394

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Root hairs show highly localized cell expansion focused to their growing tips. This growth pattern is accomplished through restriction of secretion to the elongating apex and modulation of cell wall properties, with the wall just behind the tip becoming rigidified to resist the lateral expansive forces of turgor. In this report we show that root hairs exhibit oscillating growth that is associated with oscillating increases in extracellular pH and reactive oxygen species (ROS), which lag growth by Ϸ7 s. Consistent with a role for these changes in growth control, artificially increasing extracellular pH arrested root hair elongation, whereas decreasing pH elicited bursting at the tip. Similarly, application of exogenous ROS arrested elongation, whereas scavenging of ROS led to root hair bursting. Roots hairs of the root hair-defective rhd2-1 mutant, which lack a functional version of the NADPH oxidase ATRBOH C, burst at the transition to tip growth. This phenotype could be rescued by elevating the pH of the growth medium to >6.0. Such rescued root hairs showed reduced cytoplasmic ROS levels and a lack of the oscillatory production of ROS at the tip. However, they exhibited apparently normal tip growth, including generation of the tip-focused Ca 2؉ gradient thought to drive apical growth, indicating that ATRBOH C is not absolutely required to sustain tip growth. These observations indicate that root hair elongation is coupled to spatially distinct regulation of extracellular pH and ROS production that likely affect wall properties associated with the polarized expansion of the cell.

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Section: Methodsmentioning

confidence: 99%

Oscillations in extracellular pH and reactive oxygen species modulate tip growth of Arabidopsis root hairs

Monshausen

Bibikova

Messerli

et al. 2007

Proc. Natl. Acad. Sci. U.S.A.

376

394

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“…Besides its essential role in a variety of cellular processes (reviewed by Sardans & Peñuelas, ), K is considered as a key osmolyte for wood formation (Fromm, ). K fertilization in K‐deficient soils can lead to a dramatic increase in wood biomass production in tree species such as pine (Smethurst et al ., ), poplar (Arend et al ., ; Wind et al ., ) and eucalypts (Laclau et al ., ). In these conditions, a higher cambial activity is correlated with a peak of K in the cambial zone (Kuhn et al ., ; Arend et al ., ; Langer et al ., ; Wind et al ., ; Fromm, ).…”

Section: Introductionmentioning

confidence: 98%

A systems biology view of wood formation in Eucalyptus grandis trees submitted to different potassium and water regimes

et al. 2019

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Summary Wood production in fast‐growing Eucalyptus grandis trees is highly dependent on both potassium (K) fertilization and water availability but the molecular processes underlying wood formation in response to the combined effects of these two limiting factors remain unknown. E. grandis trees were submitted to four combinations of K‐fertilization and water supply. Weighted gene co‐expression network analysis and MixOmics‐based co‐regulation networks were used to integrate xylem transcriptome, metabolome and complex wood traits. Functional characterization of a candidate gene was performed in transgenic E. grandis hairy roots. This integrated network‐based approach enabled us to identify meaningful biological processes and regulators impacted by K‐fertilization and/or water limitation. It revealed that modules of co‐regulated genes and metabolites strongly correlated to wood complex traits are in the heart of a complex trade‐off between biomass production and stress responses. Nested in these modules, potential new cell‐wall regulators were identified, as further confirmed by the functional characterization of EgMYB137. These findings provide new insights into the regulatory mechanisms of wood formation under stressful conditions, pointing out both known and new regulators co‐opted by K‐fertilization and/or water limitation that may potentially promote adaptive wood traits.

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“…Furthermore, PTORKmediated K + release might be required for charge balance during uptake of metabolites from the xylem stream, which has also been described for vessel-associated cells (Himpkamp 1988;Van Bel 1990). Vice versa, under conditions such as described in Arend et al (2004), when K + demand of the cambium is high, PTORK-expressing ray cells will import potassium from the vessels via a not yet identified K + uptake system. Our immunolocalisation data are in line with the seasonal expression profile of PTORK (Langer et al 2002), where high expression during the active growth period corresponds to the localisation in developing fibres and ray cells.…”

Section: Discussionmentioning

confidence: 89%

Polar-localised poplar K+ channel capable of controlling electrical properties of wood-forming cells

Arend¹,

Stinzing

Wind³

et al. 2005

Planta

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In previous studies, we have shown that annual expression profiles of cambial and wood tissue with respect to the Shaker K+ channel PTORK correlate with cambial activity. To follow PTORK-gene activity on the cellular level, we isolated the respective promoter regions and generated transgenic Arabidopsis plants expressing the GUS gene under the control of the PTORK promoter. Cross-sections of petioles showed PTORK-driven signals predominantly in the xylem parenchyma surrounding the vessels and in the phloem. Antibodies raised against a unique N-terminal region of PTORK in histo-immunochemical analyses recognised this K+-release channel in growth-active poplar plants only. PTORK labelling was found in differentiating xylem cells (young fibres) and mature xylem (vessel-associated cells of the ray parenchyma). Patch-clamp measurements on fibre cell protoplasts, derived from young poplar twigs, identified outward-rectifying K+ channels as the major K+ conductance of this cell type, which resembled the biophysical properties of PTORK when expressed in Xenopus oocytes.

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Effect of potassium deficiency on the plasma membrane H⁺‐ATPase of the wood ray parenchyma in poplar

Abstract: The effect of K + deficiency on the plasma membrane (PM) H

Cited by 26 publications

References 37 publications

Oscillations in extracellular pH and reactive oxygen species modulate tip growth of Arabidopsis root hairs

Oscillations in extracellular pH and reactive oxygen species modulate tip growth of Arabidopsis root hairs

A systems biology view of wood formation in Eucalyptus grandis trees submitted to different potassium and water regimes

Polar-localised poplar K+ channel capable of controlling electrical properties of wood-forming cells

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Effect of potassium deficiency on the plasma membrane H+‐ATPase of the wood ray parenchyma in poplar

Abstract: The effect of K + deficiency on the plasma membrane (PM) H

Cited by 26 publications

References 37 publications

Oscillations in extracellular pH and reactive oxygen species modulate tip growth of Arabidopsis root hairs

Oscillations in extracellular pH and reactive oxygen species modulate tip growth of Arabidopsis root hairs

A systems biology view of wood formation in Eucalyptus grandis trees submitted to different potassium and water regimes

Polar-localised poplar K+ channel capable of controlling electrical properties of wood-forming cells

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Product

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Effect of potassium deficiency on the plasma membrane H⁺‐ATPase of the wood ray parenchyma in poplar