Effect of Povidone Iodine and Hydrogen Peroxide on Fracture Healing: A Histomorphometric Study on Rats

Husodo, Kurniadi; Kamal, Achmad Fauzi; Yusuf, Achmad A.

doi:10.1177/1602400224

Cited by 7 publications

(6 citation statements)

References 32 publications

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“…Thus, 10% PVI was selected to evaluate its effect on cancellous bone union at the osteotomy site in the proximal tibia in the present study. However, 1% PVI did not cause delayed bone union in the previous study [20]. In the present study, 10% PVI, which is a higher concentration than is used at the surgical site in the clinical situation, was also used.…”

Section: Discussionmentioning

confidence: 65%

“…In an in vivo study, Husodo et al reported that 10% PVI showed a lower percentage of osseous tissue and a higher percentage of fibrous tissue, indicating delayed bone union of the osteotomy site at the femoral shaft of the rat, evaluating mainly cortical bone [20]. Thus, 10% PVI was selected to evaluate its effect on cancellous bone union at the osteotomy site in the proximal tibia in the present study.…”

Section: Discussionmentioning

confidence: 93%

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Effects of Povidone-Iodine or Ethanol Exposure on Bone Formation at the Osteotomy Site of the Proximal Tibia in Rats

Nagahata¹,

Miyakoshi²,

Kasukawa³

et al. 2019

OJO

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Background: Povidone-iodine (PVI) irrigation is currently used to decrease the frequency of postoperative surgical site infections. Ethanol (EtOH) is sometimes applied to prevent local recurrence after curettage of benign bone tumors. However, the effects of PVI and EtOH on surrounding soft tissue and on bone union are unclear. The purpose of this study was to determine whether PVI or EtOH adversely affects the cancellous bone healing of the osteotomy site at the proximal tibia in rats. Methods: A cancellous bone osteotomy was performed at the right proximal tibia in 4-month-old, female, Sprague Dawley rats. Vehicle, 10% PVI, or 95% EtOH-soaked gauze was inserted into the osteotomy site and maintained for 6 minutes. The rats were euthanized 2 or 4 weeks after the osteotomy. Results: Two weeks after treatment, the bone union rate was significantly higher in the vehicle group than in the PVI group and the EtOH group (p < 0.001). However, the bone union rate was not significantly different between the PVI and EtOH groups. There was no significant difference among the three groups in the bone union rate 4 weeks after treatment. Conclusion: PVI or EtOH delayed bone union of the cancellous bone osteotomy site of the proximal tibia in the early phase (2 weeks), but not at 4 weeks, in rats.

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Section: Discussionmentioning

confidence: 65%

Section: Discussionmentioning

confidence: 93%

Effects of Povidone-Iodine or Ethanol Exposure on Bone Formation at the Osteotomy Site of the Proximal Tibia in Rats

Nagahata¹,

Miyakoshi²,

Kasukawa³

et al. 2019

OJO

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“…The rat femur fracture model was established on a small-animal operating table on a clean bench. We referred to Husodo et al 9 for this model. Briefly, the rats were fasted for 12 h and not permitted to drink water for 3 h before anesthesia.…”

Section: Methodsmentioning

confidence: 99%

A Preliminary Exploration of the Efficacy of Gentamicin Sponges in the Prevention and Treatment of Wound Infections

Li¹,

Zhou²

2021

IDR

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This study aimed to construct drug-loading and drug-releasing quantitative equations for gentamicin sponges in addition to realizing a gentamicin sponge for wound infection prevention and treatment. Methods: Sterile sponges were cut into pieces of 1×1 × 0.5 cm and immersed in 40, 16, 8, 4, 1.6, 0.8, or 0 mg/mL of gentamicin solution for 12, 24, 48, 96, or 120 h to evaluate their gentamicin loading. The sponges were subsequently immersed in the gentamicin solution of different concentrations for 48 h, air-dried, and then immersed in 10 mL of 0.9% physiological saline to evaluate the gentamicin release. Methicillin-sensitive Staphylococcus aureus (MSSA) and Pseudomonas aeruginosa were used to explore the sponges' infection prevention scheme. In addition, a rat femur fracture with wound infection model was used to assess the infection treatment scheme. Results: The antibacterial zone sizes of the sponges immersed in 40, 16, 8, 4, 1.6, and 0.8 mg/mL of the gentamicin solution were larger than those of the 0 mg/mL air-dried sponge, and the difference was statistically significant (p < 0.01, p < 0.01, p < 0.01, p < 0.01, p < 0.01, and p < 0.01, respectively). The rats in the 40, 16, and 8 mg/mL air-dried sponge groups had no wound suppuration in either the MSSA or P. aeruginosa rat infection models. Conclusion:A quantified equation for the sponges' gentamicin loading and release was achieved with high accuracy. Furthermore, we recommend the 40, 16, or 8 mg/mL air-dried sponge for the treatment of wounds with antibiotic-sensitive bacterial infections.

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“…The rat femur fracture model was established on a small animal operating table in a super clean bench. We refer to Husodo, K. et al [9] for the rat femur fracture model. Brie y, the rats were fasted for 12 h and banned from drinking water for 3 h before anesthesia.…”

Section: Animalsmentioning

confidence: 99%

Gentamicin Sponge for Anti-infection, a Controversial Old Topic and Preliminary Exploration of the Solution, in Vitro and in Vivo Experiments

Wang

Zhou

2021

Preprint

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Purpose: Our study was to construct drug-loading and drug-release quantitative equation of gentamicin sponge, in addition, obtain the wound infection prevented and treated scheme of gentamicin sponge. Methods: Sterile sponge was cut into 1×1×0.5cm size and immersed into 40mg/ml, 16mg/ml, 8mg/ml, 4mg/ml, 1.6mg/ml, 0.8mg/ml or 0mg/ml gentamicin solution for 12h, 24h, 48h, 96h or 120h to evaluate gentamicin-loading of sponge. Sponge was immersed in gentamicin solution of different concentrations for 48h and then air-dried. Air-dried sponge was immersed into 10ml 0.9% physiological saline to evaluated gentamicin-release. Staphylococcus aureus (MSSA) and Pseudomonas aeruginosa (P. aeruginosa) were used to explore infection prevented scheme of gentamicin sponge. Besides, femur fractured with wound infection rat model was used to discuss the infection treated scheme.Results: The equation of gentamicin-loading of sponge was: z=(0.03718±0.01672)x+(-4.578e-4±0.06253)y+(-2.50935e-4±1.47521e-4)x2+(0.00303±0.00149)y2+(0.00408±3.52827e-4)xy (R2 was 0.97) and drug-release equation was z=(4.37205±1.18048)x+(-7.05921±3.09628)y+(-0.04596±0.01287)x2+(0.3309±0.07912)y2+(0.31559±0.02754)xy (R2 was 0.95). The antibacterial zone sizes of sponges immersed in 40mg/ml, 16mg/ml, 8mg/ml, 4mg/ml, 1.6mg/ml, 0.8mg/ml gentamicin solution were larger than 0mg/ml air-dried sponge, and the difference had statistically significant (p<0.01). Rats of groups of 40mg/ml air-dried sponge, 16mg/ml air-dried sponge, 8mg/ml air-dried sponge had no wound suppuration in both MSSA and P. aeruginosa infection rat model.Conclusions: The quantified equation of gentamicin-loading and release of sponge was with high accuracy. 1.6 mg/ml air-dried sponge and 0.8mg/ml air-dried sponge were enough to prevent wound infection. Besides, if wound were confirmed to be sensitive bacteria infected, we recommended to use 40mg/ml air-dried sponge, 16mg/ml air-dried sponge or 8mg/ml air-dried sponge to treat.

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Effect of Povidone Iodine and Hydrogen Peroxide on Fracture Healing: A Histomorphometric Study on Rats

Abstract: The 1% povidone iodine solution is recommended as the irrigation adjuvant in fracture surgery.

Cited by 7 publications

References 32 publications

Effects of Povidone-Iodine or Ethanol Exposure on Bone Formation at the Osteotomy Site of the Proximal Tibia in Rats

Effects of Povidone-Iodine or Ethanol Exposure on Bone Formation at the Osteotomy Site of the Proximal Tibia in Rats

A Preliminary Exploration of the Efficacy of Gentamicin Sponges in the Prevention and Treatment of Wound Infections

Gentamicin Sponge for Anti-infection, a Controversial Old Topic and Preliminary Exploration of the Solution, in Vitro and in Vivo Experiments

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