Effect of pre-freeze semen quality, extender and cryoprotectant on the post-thaw quality of Asian elephant (Elephas maximus indicus) semen

Imrat, Podjana; Suthanmapinanth, P; Saikhun, Kulnasan; Mahasawangkul, Sittidet; Šoštarić, Edita; Sombutputorn, P.; Jansittiwate, Saran; Thongtip, Nikorn; Pinyopummin, Anuchai; Colenbrander, B.; Holt, William V.; Stout, T.A.E.

doi:10.1016/j.cryobiol.2012.11.003

Cited by 21 publications

(7 citation statements)

References 43 publications

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“…Indeed, many aspects of semen composition, including volume and pH, vary between individual bulls and ejaculates following collection by transrectal massage (Imrat et al, 2013).…”

Section: Discussionmentioning

confidence: 99%

Effect of alternate day collection on semen quality of Asian elephants (Elephas maximus) with poor initial fresh semen quality

Imrat

Mahasawangkul²,

Thitaram

et al. 2014

Animal Reproduction Science

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In captivity, male Asian elephants often yield poor quality semen after transrectal manually assisted semen collection; however, the reasons for the disappointing semen quality are not clear. Here we test the hypothesis that accumulation of senescent spermatozoa is a contributory factor, and that semen quality can therefore be improved by more frequent ejaculation. To this end we investigated the effect of collecting semen five times on alternate days, after a long period of sexual rest, on semen quality in Asian elephants known to deliver poor semen during infrequent single collections. All eight bulls initially displayed a high incidence of detached sperm heads and low percentages of motile (close to 0%) spermatozoa. After semen collection on alternate days, the percentages of detached sperm heads, and head and mid-piece abnormalities, were reduced significantly (p<0.05). In particular, one bull showed markedly improved sperm motility (increased from 0% to 60%) and membrane integrity (increased from 5% to 75%). In addition, advancing age significantly (p<0.01) correlated with lower percentages of sperm with intact membranes and a higher frequency of detached sperm heads. In contrast to sperm accumulation problems in other species, a small ampullary diameter correlated significantly (p<0.05) with reduced semen quality.

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“…Indeed, many aspects of semen composition, including volume and pH, vary between individual bulls and ejaculates following collection by transrectal massage (Imrat et al, 2013).…”

Section: Discussionmentioning

confidence: 99%

Effect of alternate day collection on semen quality of Asian elephants (Elephas maximus) with poor initial fresh semen quality

Imrat

Mahasawangkul²,

Thitaram

et al. 2014

Animal Reproduction Science

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“…But in the isochoric (constant volume) process the metabolic rate of frizzing constantly changing with every 10 degrees of temperature reduction. In the isochoric process, it is possible to store at absolute zero temperature and it helps to maintain cell integrity during slow frizzing [14].…”

Section: Isochoric Cryopreservationmentioning

confidence: 99%

Cryopretectants and Their Usage in Cryopreservation Process

Bhattacharya¹

2018

Cryopreservation Biotechnology in Biomedical and Biological Sciences

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Cryoprotectants are basically some chemical compounds which prevent cells or tissues from damage due to freezing. Mostly vitrification and thawing process are mostly used in cryopreservation. Now a day's cryopreservation is becoming an apex technology for Health sector, as many organ and tissue transplant methods has been developed in recent time. Organs or tissues need to preserve properly before implantation, hence cryoprotectants plays a key role modern cryonics. This article seeks to provide answers for how cryoprotectants works and prevent tissue damage from shrinking ice crystals during freeze drying or vitrification. The various animal tissues, organs, lymphocytes, cartilages, bone marrow, proteins can be stored by using proper cryoprotectants. Optimization of cryoprotectants is a challenging task, as elevated concentration may cause cytotoxicity. This article also highlights the implication, challenges and recent advances of cryoprotectants along with its types. The importance of cryobank system and its importance were also being emphasized in this article. Further, importance of cryoprotectants in nano suspension preparation was also been discussed in this article.

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“…44 Tris and TES are among the most employed diluents used in semen preservation, 45 while coconut water has been used in the capuchin monkey, 46 the agouti, 47 and the collared peccary. 4 In addition, milk-based cryoextenders have also been used to successfully freeze the sperm from species such as the Asian elephant (Elephas maximus indicus) 48 and the Indian rhinoceros (Rhinoceros unicornis). 49 In searching for an ideal diluent for semen cryopreservation, many additives have been tested with the aim of fostering increased sperm viability after thawing.…”

Section: The Preservation Of Male Gametesmentioning

confidence: 99%

Cryopreservation in mammalian conservation biology: current applications and potential utility

Silva¹,

Lima²,

Peixoto³

et al. 2015

RRBS

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A rapid and continuous decline in wild mammalian species populations has been documented in recent decades. Although in situ conservation strategies such as habitat preservation are usually the best way to preserve biodiversity, other rescue strategies such as germplasm cryopreservation are sometimes necessary. Germplasm cryopreservation involves the freezing of gametes, embryos, gonadal tissues, or somatic tissues of species threatened with extinction. There is substantial diversity in the cryobiological requirements among cell types and tissues of each species. Research has focused on adapting techniques developed for the conservation of the genetic material of domestic animals for use with wild species about which relatively little is known. This review describes and discusses the current and potential use of cryobanking for the preservation of cells and tissues of threatened species.

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Effect of pre-freeze semen quality, extender and cryoprotectant on the post-thaw quality of Asian elephant (Elephas maximus indicus) semen

Cited by 21 publications

References 43 publications

Effect of alternate day collection on semen quality of Asian elephants (Elephas maximus) with poor initial fresh semen quality

Effect of alternate day collection on semen quality of Asian elephants (Elephas maximus) with poor initial fresh semen quality

Cryopretectants and Their Usage in Cryopreservation Process

Cryopreservation in mammalian conservation biology: current applications and potential utility

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