Uniaxial tensile tests were conducted on AISI 316LN austenitic stainless steel from-40C to 300C at a rate of 0.5 mm/min. Microstructure and mechanical properties of the deformed steel were investigated by optical, scanning and transmission electron microscopies, X-ray diffraction, and microhardness testing. The yield strength, ultimate tensile strength, elongation and microhardness increase with the decrease of the test temperature. The tensile fracture morphology has the dimple rupture feature after low temperature deformations, and turns to a mixture of transgranular fracture and dimple fracture after high temperature ones. The dominating deformation microstructure evolves from dislocation tangle/slip bands to large deformation twins/slip bands with temperature decrease. The deformation-induced martensite transformation can only be realized at low temperature, and its quantity increases with the decrease of the temperature.