Effect of prednisolone therapy on serum levels of 1,2‐O‐dilauryl‐rac‐glycero glutaric acid‐(6′‐methylresorufin) ester lipase in dogs

Abstract

Background: Activity of 1,2-O-dilauryl-rac-glycero glutaric acid-(6 0-methylresorufin) ester (DGGR) lipase in serum shows good agreement with pancreatic lipase (cPL) in dogs. Although prednisolone therapy does not seem to affect serum cPL concentration, its influence on DGGR lipase is unclear. Objectives: The aim of the study was to evaluate the effect of prednisolone therapy on DGGR lipase serum activity in dogs. Animals: Thirty-four dogs were used, of which 17 dogs received prednisolone (study group) and 17 healthy dogs did not receive treatment.

Advances in the diagnosis of acute pancreatitis in dogs

“…Given that exogenous glucocorticoids cause only minor increases in Spec cPL (Texas A&M University, Gastrointestinal Laboratory) and DGGR‐lipase, we suspect that increased lipase concentrations in dogs with HAC reflect subclinical pancreatic injury of unknown clinical relevance. 92 , 93 , 94 This occult pancreatitis hypothesis is supported by a study reporting pancreatic hyperechogenicity in dogs with HAC. 95 Although a hyperechoic pancreas is not a feature of AP, hyperechogenicity is a suspected ultrasonographic feature of CP in dogs based on anecdotal reports, data from a case series, and experimental models.…”

“…Increased DGGR lipase and 1,2 DiG lipase concentrations also have been reported in 22 dogs with HAC and no clinical or ultrasonographic signs of AP. Given that exogenous glucocorticoids cause only minor increases in Spec cPL (Texas A&M University, Gastrointestinal Laboratory) and DGGR‐lipase, we suspect that increased lipase concentrations in dogs with HAC reflect subclinical pancreatic injury of unknown clinical relevance 92‐94 . This occult pancreatitis hypothesis is supported by a study reporting pancreatic hyperechogenicity in dogs with HAC 95 .…”

Progression of lipase activity and pancreatic lipase immunoreactivity in dogs hospitalized for acute pancreatitis and correlation with clinical features

“…Prednisolone can increase PLI concentrations into a diagnostic range for pancreatitis in healthy dogs, 47 whereas effects on DGGR‐based lipase activity results are minimal and mostly within the RI. 48 We purposely did not exclude these dogs, because we were curious if lipase activity and PLI would be affected differently. As can be seen from Figure 2A,B (corticosteroid‐treated dogs are marked orange), such was not the case.…”

New insights into the etiology, risk factors, and pathogenesis of pancreatitis in dogs: Potential impacts on clinical practice

“…Administration of corticosteroids prior to referral also had no effect on serum cPLI activities in a recent study of dogs with intervertebral disc disease 73 . The effect of exogenous corticosteroids on 1,2‐O‐dilauryl‐rac‐glycero glutaric acid‐(6′‐methylresorudin) ester (DGGR) lipase assays has also been evaluated 74 . In 17 dogs being treated with corticosteroids for several reasons DGGR lipase activity increased following initiation of treatment and reduced during drug tapering 74 .…”

“…74 In 17 dogs being treated with corticosteroids for several reasons DGGR lipase activity increased following initiation of treatment and reduced during drug tapering. 74 The magnitude of increase in DGGR lipase activity was however low, potentially reducing the clinical significance of this finding. 74 The combined results of the above studies suggest that exogenous corticosteroids are unlikely to result in clinically important AP in dogs, although markers of subclinical pancreatic injury/inflammation might be noted in some cases, and might be more importantly influenced by concurrent disease.…”