Effect of Prednisone Treatment on the Human Immune Response to Keyhole Limpet Hemocyanin

Tuchinda, M; Newcomb, Richard W.; DeVald, B. L.

doi:10.1159/000230634

Cited by 64 publications

(16 citation statements)

References 7 publications

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“…Recently, it has been reported [17] that (alternate day) therapy with prednisone was associated with an en hanced primary response (at 2 weeks) in asthmatic children immunized with keyhole limpet hcmocyanin (KLH). Since other asthmatic children not receiving prednisone showed a smaller antibody response to KLH at 2 weeks, the results suggest that corticosteroids may have an augmenting effect on the primary immune response, at least in this group of patients.…”

Section: Discussionmentioning

confidence: 99%

Effect of Hydrocortisone on Immunoglobulin Synthesis and Secretion by Human Peripheral Lymphocytes <i>in vitro</i>

Smith¹,

Sherman²,

Middleton³

1972

Int Arch Allergy Immunol

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Human peripheral lymphocytes in tissue culture synthesize immunoglobulin Ig which is secreted into the medium. The small amounts of Ig produced can be detected in nanogram quantities by a modification of the ammonium sulfate test. Ig formation by peripheral human lymphocytes was affected by hydrocortisone when the cells were briefly exposed to the hormone at the onset of incubation. When hydrocortisone was dissolved in culture medium or when the more soluble hydrocortisone-21-sodium succinate was employed, significantly increased in vitro synthesis of Ig was noted at 10^––⁹ to 10^––5 and 10^––10 to 10^––6 M, respectively (both p < 0.01). With hydrocortisone-21-sodium succinate, in the concentration range 10^––5 to 10^––3_M, no stimulation of Ig synthesis was observed and at 10^––3 M there was decreased Ig synthesis compared to the controls. Average lymphocyte viability at all concentrations was in the range of 39–52% but at the highest concentrations of hydrocortisone tended to be somewhat less, but not significantly. Similar results were obtained when hydrocortisone was dissolved in ethanol but lymphocyte viability was more variable in these experiments. Radioimmunoelectrophoretic and radioimmunodiffusion analysis of culture supernatants from culture including ¹⁴C-l-leucine showed that the only Ig formed was IgG; a β-globulin was also formed (radioimmunoelectrophoresis). These experiments demonstrate that human peripheral lymphocytes cultured in vitro interact with hydrocortisone to modify Ig synthesis.

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Section: Discussionmentioning

confidence: 99%

Effect of Hydrocortisone on Immunoglobulin Synthesis and Secretion by Human Peripheral Lymphocytes <i>in vitro</i>

Smith¹,

Sherman²,

Middleton³

1972

Int Arch Allergy Immunol

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“…When corticosteroids inhibit a suppressor infl uence, antibody production may actually increase. Enhancement of antibody production by cortico steroid administration has been reported in animals (84) and man (85). The addition of pharmacologic or physiologic concentrations of corticosteroid to an in vitro system of antibody production by human B lymphocytes inhibited the negative effect and produced an enhanced antibody response (86).…”

Section: Monocyte-macrophagesmentioning

confidence: 99%

Mechanisms of Glucocorticoid Action on Immune Processes

Parrillo¹,

Fauci²

1979

Annu. Rev. Pharmacol. Toxicol.

379

115

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“…More recent findings suggest that high dose administration of steroid results in modest decreases in serum immunoglobulins, particularly IgG and IgA, which may persist for periods of several months after treatment is stopped (5,6). Specific antibody synthesis in man, however, has not been shown to be suppressed by corticosteroids, and in one study, enhancement was demonstrated (7). In young chickens, treatment with cortisone resulted in the mobilization of B lymphocytes from the Bursa and enhanced the differentiation into mature immunoglobulin secreting plasma cells in the peripheral lymphoid tissues (8).…”

Section: Introductionmentioning

confidence: 99%

Immunoglobulin production induced in vitro by glucocorticoid hormones: T cell-dependent stimulation of immunoglobulin production without B cell proliferation in cultures of human peripheral blood lymphocytes.

Grayson¹,

Dooley²,

Koski³

et al. 1981

J. Clin. Invest.

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A B S T R A C T The direct effects of steroid hormones on the production of immunoglobulins and DNA synthesis by human T and B lymphocytes was evaluated in cultures of peripheral blood mononuclear cells. As detected by a reverse hemolytic plaque assay, the addition of 0.1 mM to 10 nM hydrocortisone to lymphocytes in culture in the absence of other stimulants or mitogens, resulted in the dramatic induction of immunoglobulin production with responses comparable to those seen in similar cultures stimulated with pokeweed mitogen. Steroid-stimulated immunoglobulin production was first seen after 48 h and peaked at 8-10 d of culture. The production of IgG, IgA, and IgM was induced following incubation with steroid. Glucocorticoids, but not estrogens or androgens, were capable of mediating this effect, and only compounds with affinity for the glucocorticoid receptor were active. The induction of immunoglobulin production was dependent on both T cells and monocytes; cultures depleted of either cell type did not produce immunoglobulin when stimulated with glucocorticoid hormones. Proliferation of B cells or T cells could not be detected by [3H]thymidine incorporation or total cell recovery from steroid-stimulated cultures, even though such cultures demonstrated marked increases in immunoglobulin production. The mechanism responsible for this functional maturation of B cells to become high rate immunoglobulin producing cells is as yet undefined, although it appears to involve more than merely steroid mediated inactivation of suppressor T cells.

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Effect of Prednisone Treatment on the Human Immune Response to Keyhole Limpet Hemocyanin

Cited by 64 publications

References 7 publications

Effect of Hydrocortisone on Immunoglobulin Synthesis and Secretion by Human Peripheral Lymphocytes <i>in vitro</i>

Effect of Hydrocortisone on Immunoglobulin Synthesis and Secretion by Human Peripheral Lymphocytes <i>in vitro</i>

Mechanisms of Glucocorticoid Action on Immune Processes

Immunoglobulin production induced in vitro by glucocorticoid hormones: T cell-dependent stimulation of immunoglobulin production without B cell proliferation in cultures of human peripheral blood lymphocytes.

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