Effect of preovulatory follicle maturity on pregnancy establishment in cattle: the role ofoocyte competence and the maternal environment

Dickinson, Sarah; Geary, T. W.; Monnig, J. M; Pohler, Ky G; Green, Jonathan A.; Smith, Michael F.

doi:10.21451/1984-3143-ar879

Cited by 11 publications

(3 citation statements)

References 53 publications

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“…Several reports have revealed the potential impacts of EB on cattle reproduction. In the absence of the corpus luteum, the administration of estradiol could elevate preovulatory estradiol concentrations, which are important for follicular development, ovulation success, and the establishment of pregnancy [34][35][36]. Ovariectomized cows treated with estradiol before embryo transfer were more likely to maintain a pregnancy until day 29 than ovariectomized cows not treated with estradiol [37].…”

Section: Discussionmentioning

confidence: 99%

Administration of Estradiol Benzoate Enhances Ovarian and Uterine Hemodynamics in Postpartum Dairy Buffaloes

Samir

El‐Sherbiny

Ahmed

et al. 2023

Animals

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The postpartum (PP) period is a crucial stage for the resumption of reproductive performance and ovarian cyclicity in dairy buffaloes. The present study aimed, for the first time, to assess the effect of the administration of estradiol benzoate (EB) on ovarian and uterine hemodynamics in PP dairy buffaloes. Eight pluriparous acyclic domestic buffaloes were enrolled in the present experiment and received a dose of 10 mg of estradiol benzoate (EB) intramuscularly 4 weeks after parturition. All animals were examined two times before EB administration (days −3, and −1) and on the day of EB administration (day 0), followed by examinations on days 1, 3, 5, 7, and 9 post-EB administration. The middle uterine artery (MUA) and ovarian artery (OA) blood flow patterns were assessed using a color Doppler ultrasound device. The reproductive parameters were (1) the cross-sectional diameters (cm) of the OA and MUA, (2) cranial uterine horn thickness (UHT; cm), and (3) hemodynamic changes within the MUA on both the ipsi- and contra-lateral sides of the previous pregnant horn and within the OA corresponding to the ovarian tissues. The examined blood flow parameters were the pulsatility index (PI), resistance index (RI), peak systolic/end-diastolic ratio (S/D), time-averaged maximum velocity (TAV; cm/s), uterine blood flow rate (BFR; bpm), and uterine blood flow volume (BFV; mL/min). Concomitantly, blood samples were collected from the coccygeal vein, and the sera were stored at −18 °C for use in estradiol (E2-17β) and nitric oxide (NO) assays. The results revealed increases in both OA and MUA cross-sectional diameter (cm) on the ipsi-lateral and contra-lateral (p < 0.05) sides within 24 h until day 9 post-treatment. The values of the RI and PI of blood flow within the OA and MUA on the ipsi-lateral and contra-lateral sides of the previous pregnancy were obviously lower (p < 0.05) at 24 h after the administration of EB, and then, started to gradually elevate, reaching the pre-treatment values on day 9 after EB administration. Both the BFR and BFV in the OA and MUA significantly increased from 24 h to 72 h after EB administration on both the ipsi-lateral and contra-lateral sides (p < 0.05); then, their values started to decrease to reach the pretreatment value on day 9 after EB administration. Both E2 and NO concentrations significantly increased (p < 0.05) from 24 h until day 3 after EB injection and then started to decline after that, reaching the pre-treatment value on day 9. In conclusion, the administration of EB enhances the ovarian and uterine blood flow concomitantly with increased levels of NO in PP dairy buffaloes.

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Section: Discussionmentioning

confidence: 99%

Administration of Estradiol Benzoate Enhances Ovarian and Uterine Hemodynamics in Postpartum Dairy Buffaloes

Samir

El‐Sherbiny

Ahmed

et al. 2023

Animals

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“…When pre-ovulation estradiol-17β (E2) concentrations and post-ovulation progesterone concentrations were higher, all of which had a favorable impact on the percentage of pregnant postpartum cows (Ciernia et al, 2021). A lower plasma estradiol-17β (E2) concentration before and after ovulation, as well as lower plasma progesterone concentration after ovulation, can result in insufficient preparation of the maternal tissues for pregnancy (Pohler et al, 2012;Dickinson et al, 2016). The two most significant factors influencing the proportion of cows pregnant were estradiol concentrations prior to a GnRH-induced ovulation (d 0) and progesterone concentrations on d 7 after ovulation (Atkins et al, 2013;Jinks et al, 2013).…”

Section: Concentrations Of Progesterone (Ng/ml) and Estradiol-17β (Pg/ml)mentioning

confidence: 99%

Influence of Mastitis on Resumption of Ovarian Activity and ‎postpartum Reproductive Measurements in Baladi Cows

Damarany

2021

Egyptian Journal of Animal Production

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“…The rapid decrease in circulating concentrations of progesterone permits an increase in luteinizing hormone (LH) pulse frequency (Goodman and Karsch, 1980), which stimulates an increase in estradiol secretion from the follicle wall via the two cell-two gonadotropin concept. Preovulatory concentrations of estradiol coordinate a series of events essential to the establishment of pregnancy, including gamete transport, induction of the preovulatory gonadotropin surge, estrous behavior, induction of progesterone receptors in the endometrium, and timing of endometrial PG secretion (reviewed by Dickinson et al, 2016). Following ovulation, increased concentrations of progesterone affect histotroph production and conceptus elongation (reviewed by Brooks et al, 2014).…”

Section: Introductionmentioning

confidence: 99%

Effect of preovulatory follicle size on cumulus cell and follicular wall transcript abundance in beef cows

Monnig¹

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Gonadotropin releasing hormone (GnRH)-induced ovulation of small dominant follicles decreased pregnancy rates and increased late embryonic/fetal mortality in postpartum beef cows, which could be caused by inadequate oocyte competence and(or) maternal environment. Previous studies revealed that dominant follicle size at GnRHinduced ovulation in beef cows may affect oocyte competence, as higher fertilization rates and higher embryo quality were achieved as dominant follicle diameter at insemination increased. In addition, higher pregnancy rates and increased concentrations of circulating preovulatory estradiol and postovulatory progesterone were observed in recipient cows induced to ovulate large compared to small dominant follicles, which may affect the establishment of pregnancy. The objectives of the current study were to determine the effects of preovulatory follicle size and physiological status on the cumulus cell transcriptome (Experiment 1) and determine the effects of preovulatory follicle size and steroidogenic capacity (i.e. concentrations of circulating estradiol) on the dominant follicle wall transcriptome collected before the preovulatory gonadotropin surge (Experiment 2). In Experiment 1, ovulation was synchronized in suckled beef cows by administering an injection of GnRH on d-9, prostaglandin F2a (PG) on d-2, and a second injection of GnRH on d0. The dominant follicle was trans-vaginally aspirated on d1 and cumulus-oocyte complexes (COC) were collected. The cumulus cells were removed from the oocytes and assigned to one of the following follicle classifications based on follicle diameter and estrus expression: small (n=6; less than 11.7mm; no estrus expression), large (n=6; greater than 12.5 mm; no estrus expression), or spontaneous (n=5; 11.6-13.9 mm; estrus expression and endogenous gonadotropin surge). RNA was extracted from cumulus cells collected from pools of four oocytes and sequenced before being aligned to the Bos taurus genome (UMD3.1). When comparing the cumulus cell transcriptome of small versus large preovulatory follicles, 430 transcripts were more abundant at a false discovery rate (FDR) less than 0.10 in small follicles and 454 were more abundant in large follicles. The glycolytic pathway was enriched in the cumulus cells of large follicles compared to small follicles. In the small versus spontaneous preovulatory follicle comparison, 597 transcripts were more abundant in small follicles and 1012 transcripts were more abundant in spontaneous follicles. The steroid biosynthesis pathway (i.e. cholesterol synthesis) was enriched in the cumulus cells of spontaneous compared to small follicles. In the large versus spontaneous preovulatory follicle comparison, 541 transcripts were more abundant in cumulus cells from large follicles and 951 were more abundant in cumulus cells from spontaneous follicles. In summary, a greater abundance of transcripts encoding for members of the glycolytic pathway in large follicles and transcripts encoding for members of the steroid biosynthesis pathway in the cumulus cells of spontaneous compared to small follicles indicate that oocytes from small follicles may be less competent. Preovulatory follicle development was synchronized in Experiment 2 by administering GnRH on d-9 and PG on d-2 to non-lactating beef cows. The ovaries were harvested at slaughter 48 hr after PG (d0) and the dominant follicle collected. RNA was extracted from approximately half of the follicle wall and sequenced. Cows were divided into two classifications based on dominant follicle diameter at collection: small (n=4; less than 11.5 mm) and large (n=7; greater than 12.5 mm). Nine transcripts were more abundant in small follicles, and two transcripts were more abundant in large follicles. No significant pathways were found. The same 11 follicle walls were also divided into two classifications based on concentration of serum estradiol at 48 hr after PG: low (n=6; less than 4.0 pg/ml) and high (n=5; greater than or equal to 4.0 pg/ml). In the low estradiol classification, 281 transcripts were more abundant in the follicle wall and 40 were more abundant in the follicle wall of the high estradiol classification. No significant pathways were found. Transcripts were analyzed with PANTHER to find significant gene ontology (GO) terms. Differentially abundant transcripts in low estradiol follicles were more highly associated with mitosis, chromosome segregation, and regulation of biological processes. In summary, a small number of transcripts were differentially abundant in the follicle wall of small versus large dominant follicles prior to the preovulatory gonadotropin surge and no specific pathways were identified that might provide insight into how the physiological maturity of a dominant follicle can affect pregnancy rate. Comparisons made between follicle walls from follicles with low or high serum estradiol found a larger number of transcripts were more highly abundant in the low estradiol follicles, possibly indicating that a higher level of transcription is taking place in the low estradiol follicles, and therefore are less mature.

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Effect of preovulatory follicle maturity on pregnancy establishment in cattle: the role ofoocyte competence and the maternal environment

Cited by 11 publications

References 53 publications

Administration of Estradiol Benzoate Enhances Ovarian and Uterine Hemodynamics in Postpartum Dairy Buffaloes

Administration of Estradiol Benzoate Enhances Ovarian and Uterine Hemodynamics in Postpartum Dairy Buffaloes

Influence of Mastitis on Resumption of Ovarian Activity and ‎postpartum Reproductive Measurements in Baladi Cows

Effect of preovulatory follicle size on cumulus cell and follicular wall transcript abundance in beef cows

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