Effect of presence of <i>corpora lutea</i> on cumulus expansion of <i>in vitro</i> matured bovine oocytes selected by trypan blue and brilliant cresyl blue tests

Quezada‐Casasola, Andrés; Martínez-Armendáriz, Karla Eugenia; Itzá-Ortíz, Mateo; Escárcega‐Ávila, Angélica María; Pérez-Eguía, Eduardo; Filipiak, Y.; Larocca, C.; Carrera‐Chávez, José Maria

doi:10.1080/09712119.2018.1440566

Cited by 8 publications

(4 citation statements)

References 45 publications

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“…Some studies have shown that the corpus luteum in an ovary enhances the quality and developmental competence of oocytes derived from that ovary (Reis et al, 2006;Moreno et al, 1993;Penitente-Filhe et al, 2015). However, our study findings contrast those of other studies (Shabankareh et al, 2015;Quezada-Casasola et al, 2018), as well as subsequent embryonic development (Gonzalez-Bulnes et al, 2005;Manjunatha et al, 2008;Boediono et al, 1995;Pirestani et al, 2011). Additionally, several studies have reported no effects (de W it et al, 2000;Sungulle, 2008).…”

Section: Morphometric Assessment Of the Bovine Ovary For In Vitro Mat...contrasting

confidence: 99%

Morphometric Assessment of the Bovine Ovary for in vitro Matured Oocyte Quality to Determine Developmental Competence

Ammari

Amran

Ghadi

et al. 2022

IJAR

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Background: Embryo production in vitro requires three steps: in vitro maturation (IVM), in vitro fertilization (IVF) and in vitro culture (IVC). The first step in the in vitro maturation of oocytes is germinal vesicle breakdown (GVBD), followed by completion of the 1st meiotic division and formation of the 1st polar body. These parameters are critical during ovary and oocyte selection. This study aimed to evaluate bovine ovary and oocyte collection for IVM. Methods: Ovaries collected from cows in a 0.9% NaCl saline solution at the central slaughterhouse were divided into two normal and abnormal ovaries according to their morphological appearance. The morphometric dimensions of bovine ovaries, such as weight and deamination (length, width and volume), were recorded. The oocyte was extruded and its deamination occurred before and after culture in maturation medium. Result: The mean ovary weight, volume, length and width were not significantly different between the two ovary types. Additionally, the dominant and subordinate follicle diameters in both ovary types showed no significant differences. Furthermore, the oocyte number per ovary of the normal and abnormal oocytes showed no significant differences. The mean cumulus oocyte complex before maturation showed no significant difference (52.73±7.23 mm for the normal ovary vs. 43.015±5.41 mm for the abnormal ovary). However, after maturation, a highly significant difference was found (P less than 0.001) between the normal ovaries before and after maturation (178.10±15.36 µm) and abnormal ovaries (10.45±7.99 µm). Additionally, data analysis of oocytes with or without 1st polar bodies revealed a highly significant difference (P less than 0.001) between oocytes of the normal ovary with (32.15±4.19) and without (10.95±1.59) 1st polar bodies and oocytes of the abnormal ovary with (7.0±078) and without 1st polar bodies (3.3±0.32). Thus, the critical point at which the normal ovary produces better in vitro matured follicles with good oocyte quality and produces the 1st polar body determines developmental competence. Therefore, the best selection of normal ovaries will enhance in vitro maturation for subsequent experiments, such as in vitro fertilization or cloning and in vitro embryo development.

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Section: Morphometric Assessment Of the Bovine Ovary For In Vitro Mat...contrasting

confidence: 99%

Morphometric Assessment of the Bovine Ovary for in vitro Matured Oocyte Quality to Determine Developmental Competence

Ammari

Amran

Ghadi

et al. 2022

IJAR

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“…Fertilization success in in vitro embryo production is typically high (~90%), but a significant proportion of embryos fail to develop to blastocysts (Carter et al, 2008;Lonergan et al, 2016). The environment of in vitro culture to which the embryo is exposed is crucial in determining the rate of embryos to develop further stages (Quezada-Casasola et al, 2018;Sen and Kuran, 2018). Most studies have agreed that an elevated concentration of circulating progesterone in vivo is associated with an increase in pregnancy rate in cattle (Stronge et al, 2005;McNeill et al, 2006).…”

Section: Resultsmentioning

confidence: 99%

Effects of Progesterone on in Vitro Developmental Competence of Bovine Embryos

Örnek

Güzey

2020

Turkish JAF Sci.Tech.

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Progesterone plays a key role in the establishment and maintenance of pregnancy in mammalian. Increasing levels of circulating progesterone in the post-conception period are associated with conceptus elongation and high pregnancy rates in cattle. Contradictory results are available on the direct role of progesterone in early embryo development. The objective of this study was to evaluate direct effects of progesterone on in vitro development of cattle embryos. Immature oocytes collected from slaughtered animals and cultured in the presence of different concentrations of progesterone (25, 50, 100 ng/mL) following in vitro fertilization. Cleavage rates in 25 and 50 ng/mL concentrations of progesterone were significantly higher than those in controls and 100 ng/mL. Rate of embryos that reached to the morula stage was similar in all groups. Supplementation of 25 and 50 ng/mL progesterone to the culture media significantly increased blastocyst yield while 100 ng/mL progesterone resulted in a decrease. As a conclusion, we can suggest that progesterone supplementation in in vitro culture may support embryo development at low levels.

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“…The presence of a corpus luteum (CL) and/or progesterone in mice affects estradiol concentration, which is required for the acquisition and maintenance of CCs to undergo expansion [42]. In bovines, the presence of a CL in the ovary exerts a negative effect on the expansion of CCs, suggesting that the CL receives the highest rate of blood flow out of all the other ovarian tissues [43].…”

Section: Discussionmentioning

confidence: 99%

GDF-9 and BMP-15 mRNA Levels in Canine Cumulus Cells Related to Cumulus Expansion and the Maturation Process

Ramirez

Palomino

Aspee

et al. 2020

Animals

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The competence to undergo expansion is a characteristic of cumulus cells (CCs). The aim was to investigate the expression of GDF-9 and BMP-15 mRNA in canine cumulus cells in relation to cumulus expansion and meiotic development over the estrous cycle. CCs were recovered from nonmatured and in vitro-matured (IVM) dog cumulus oocyte complexes (COCs), which were obtained from antral follicles at different phases of the estrous cycle. Quantitative real-time polymerase chain reaction (q-PCR) was used to evaluate the relative abundance of GDF-9 and BMP-15 transcripts from the CCs with or without signs of expansion. The results were evaluated by ANOVA and logistic regression. The maturity of the oocyte and the expansion process affected the mRNA levels in CCs. There were differences (p < 0.05) in GDF-9 and BMP-15 gene expression in CCs isolated from nonmatured COCs when comparing the reproductive phases. Lower mRNA levels (p < 0.05) were observed in anestrus and proestrus in comparison to those in estrus and diestrus. In contrast, when comparing GDF-9 mRNA levels in IVM COCs, no differences were found among the phases of the estrous cycle in expanded and nonexpanded CCs (p < 0.05). However, the highest (p < 0.05) BMP-15 gene expression in CCs that did not undergo expansion was exhibited in anestrus and the lowest (p < 0.05) expression was observed in estrus in expanded CCs. Although the stage of the estrous cycle did not affect the second metaphase (MII )rates, the expanded CCs obtained at estrus coexisted with higher percentages of MII (p < 0.05). In conclusion, the differential expression patterns of GDF-9 and BMP-15 mRNA transcripts might be related to cumulus expansion and maturation processes, suggesting specific regulation and temporal changes in their expression.

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Effect of presence of corpora lutea on cumulus expansion of in vitro matured bovine oocytes selected by trypan blue and brilliant cresyl blue tests

Cited by 8 publications

References 45 publications

Morphometric Assessment of the Bovine Ovary for in vitro Matured Oocyte Quality to Determine Developmental Competence

Morphometric Assessment of the Bovine Ovary for in vitro Matured Oocyte Quality to Determine Developmental Competence

Effects of Progesterone on in Vitro Developmental Competence of Bovine Embryos

GDF-9 and BMP-15 mRNA Levels in Canine Cumulus Cells Related to Cumulus Expansion and the Maturation Process

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