Effect of pressure on biomethanation process and spatial stratification of microbial communities in trickle bed reactors under decreasing gas retention time

Ebrahimian, Farinaz; Bernardini, Nicola; Tsapekos, Panagiotis; Treu, Laura; Zhu, Xinyu; Campanaro, Stefano; Karimi, Keikhosro; Angelidaki, Irini

doi:10.1016/j.biortech.2022.127701

Cited by 28 publications

(12 citation statements)

References 52 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…With the overall objective of this study to validate the use of BTFs for biomethanation in larger scales, the results obtained in this study needs to be compared with laboratory scale studies. To meet this target, the laboratory studies from the groups within this project were used (Ashraf et al, 2021(Ashraf et al, , 2020Dahl Jønson et al, 2020;Ebrahimian et al, 2022;Ghofrani-Isfahani et al, 2022;Jønson et al, 2022;Tsapekos et al, 2021). The studies were all precursor to this pilot-scale study.…”

Section: Validation Of Pilot Scale Resultsmentioning

confidence: 99%

“…Parallel operation: The focus was to validate the technology in operational environment, compared to the previous laboratory scale studies (Ashraf et al, 2021(Ashraf et al, , 2020Dahl Jønson et al, 2020;Ebrahimian et al, 2022;Ghofrani-Isfahani et al, 2022;Jønson et al, 2022;Tsapekos et al, 2021). The BTFs were operated under identical conditions of temperature, flow, and trickling.…”

Section: Experimental Periodsmentioning

confidence: 99%

“…As this study was focused on the potential of biomethanation in a BTF for larger scale operations, the enrichment phase was important. For larger scale operations, to pre-enrich inoculum with the same method as used for the studies of Ashraf et al (2020), Ghofrani-Isfahani et al (2022), Ebrahimian et al (2022) and Tsapekos et al (2021) would be expensive in terms of operating cost and time. Using a direct inoculation with non-enriched inoculum would reduce the expenses for enrichment of the BTF.…”

Section: Non-enriched Inoculum Vs Pre-enriched Inoculummentioning

confidence: 99%

“…It should be mentioned that the inoculum taken from Bassani et al (2015) was enriched in planktonic environment and grown at GRTs above 10 h. In the present study, significantly higher gas rates were fed to achieve the targeted GRTs (<0.5 h) and, the utilized BTFs utilized that rely on biofilm formation which is a completely different reactor design. Thus, the microbiomes at the studies of Ashraf et al (2020), Ghofrani-Isfahani et al (2022), Ebrahimian et al (2022) and Tsapekos et al (2021) were subjected to more intense operating conditions and completely different environment (i.e., from planktonic to biofilm) leading to relatively prolonged lag phase. Nevertheless, high biomethanation was achieved in all the cited studies of this project.…”

Section: Non-enriched Inoculum Vs Pre-enriched Inoculummentioning

confidence: 99%

See 3 more Smart Citations

Pilot-scale study of biomethanation in biological trickle bed reactors converting impure CO2 from a Full-scale biogas plant

Jønson

Tsapekos

Ashraf

et al. 2022

Bioresource Technology

Self Cite

View full text Add to dashboard Cite

Section: Validation Of Pilot Scale Resultsmentioning

confidence: 99%

Section: Experimental Periodsmentioning

confidence: 99%

Section: Non-enriched Inoculum Vs Pre-enriched Inoculummentioning

confidence: 99%

Section: Non-enriched Inoculum Vs Pre-enriched Inoculummentioning

confidence: 99%

See 2 more Smart Citations

Pilot-scale study of biomethanation in biological trickle bed reactors converting impure CO2 from a Full-scale biogas plant

Jønson

Tsapekos

Ashraf

et al. 2022

Bioresource Technology

Self Cite

View full text Add to dashboard Cite

“…Another study found that psychrophilic conditions can inhibit methanogenic activity but either mesophilic or psychrophilic conditions can enrich homoacetogens [ 45 ]. To better understand the microbial communities and their functioning, omics techniques such as metagenomics and metatranscriptomics have been exploited to unravel in more detail the community members and their metabolic functions during biomethanation of hydrogen [ 46 , 47 , 48 , 49 , 50 , 51 , 52 , 53 ].…”

Section: Introductionmentioning

confidence: 99%

Effect of Inoculum Microbial Diversity in Ex Situ Biomethanation of Hydrogen

et al. 2022

View full text Add to dashboard Cite

The effects of the inoculum origin, temperature or operational changes on ex situ biomethanation by complex microbial communities have been investigated; however, it remains unclear how the diversity of the inoculum influences the process and its stability. We explored the effect of microbial diversity of four inocula (coded as PF, WW, S37 and Nrich) on methane production, process stability and the formation of volatile fatty acids as by-products. The highest methane amounts produced were 3.38 ± 0.37 mmol, 3.20 ± 0.07 mmol, 3.07 ± 0.27 mmol and 3.14 ± 0.06 mmol for PF, WW, S37 and Nrich, respectively. The highest acetate concentration was found in less diverse cultures (1679 mg L−1 and 1397 mg L−1 for S37 and Nrich, respectively), whereas the acetate concentrations remained below 30 mg L−1 in the more diverse cultures. The maximum concentration of propionate was observed in less diverse cultures (240 mg L−1 and 37 mg L−1 for S37 and Nrich cultures, respectively). The highly diverse cultures outperformed the medium and low diversity cultures in the long-term operation. Methanogenic communities were mainly composed of hydrogenotrophic methanogens in all cultures. Aceticlastic methanogenesis was only active in the highly diverse sludge community throughout the experiment. The more diverse the inocula, the more methane was produced and the less volatile fatty acids accumulated, which could be attributed to the high number of microbial functions working together to keep a stable and balanced process. It is concluded that the inoculum origin and its diversity are very important factors to consider when the biomethanation process is performed with complex microbial communities.

show abstract

Bioprocess Design and Technoeconomic Analysis of 2,3-Butanediol Production in Wood-Based Biorefineries

Ebrahimian,

Mohammadi

2024

Waste Biomass Valor

View full text Add to dashboard Cite

Abstract2,3-Butanediol (BDO) is a crucial precursor in various industries, traditionally derived from fossil resources, raising environmental concerns. This study evaluates the techno-economic feasibility of producing BDO from wood residues, a sustainable resource abundantly available in Nordic countries. By modeling a biorefinery plant with a daily capacity of 100 metric tons of wood chips, three scenarios (Sc.) were explored: Sc. 1, where BDO is the sole product; Sc. 2, where BDO is produced alongside methane and biofertilizer; and Sc. 3, which incorporates a combined heat and power system using biogas from the waste stream. The analysis emphasizes the minimum selling price (MSP) of BDO, revealing it to be lowest in Sc. 1 at USD2.97/kg, compared to USD3.20/kg and USD3.48/kg for Sc. 2 and Sc. 3, respectively. Notably, sensitivity analysis highlighted the impact of processing capacity on economic performance, suggesting a potential for higher scalability and profitability in Sc. 2. This study contributes novel insights into the role of processing capacity and fermentation yield in optimizing BDO production, providing a valuable framework for technology developers aiming to establish wood-based biorefineries. These findings not only enhance understanding of economic thresholds but also underscore the importance of resource efficiency and strategic planning in bio-based production setups. Graphical abstract

show abstract

Effect of pressure on biomethanation process and spatial stratification of microbial communities in trickle bed reactors under decreasing gas retention time

Cited by 28 publications

References 52 publications

Pilot-scale study of biomethanation in biological trickle bed reactors converting impure CO2 from a Full-scale biogas plant

Pilot-scale study of biomethanation in biological trickle bed reactors converting impure CO2 from a Full-scale biogas plant

Effect of Inoculum Microbial Diversity in Ex Situ Biomethanation of Hydrogen

Bioprocess Design and Technoeconomic Analysis of 2,3-Butanediol Production in Wood-Based Biorefineries

Contact Info

Product

Resources

About