Effect of pressure on membranes

Winter, Roland; Jeworrek, Christoph

doi:10.1039/b901690b

Cited by 198 publications

(246 citation statements)

References 129 publications

Supporting

Mentioning

227

Contrasting

Unclassified

Order By: Relevance

“…The main transition occurs at about 30 °C (303 K), which is 6 °C higher than in the MLVs, as expected, since the membranes are less hydrated [17,41,53].…”

Section: Mlbsmentioning

confidence: 54%

“…Experimentally, mainly the mechanisms behind lipid phase transitions have been investigated using methods such as calorimetry [8], electron paramagnetic resonance [9], dilatometry [10,11,12], light transmittance [13,14], Fourier transform infrared spectroscopy (FTIR) [15] and small angle scattering of neutrons (SANS) or X-rays (SAXS) [16,17]. Neutron and X-ray diffraction [18], spectroscopy [19], reflectometry [20] and solid-state NMR [21] have also given information on the structure and dynamics of model lipid membranes.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Direct comparison of elastic incoherent neutron scattering experiments with molecular dynamics simulations of DMPC phase transitions

et al. 2016

View full text Add to dashboard Cite

“…The main transition occurs at about 30 °C (303 K), which is 6 °C higher than in the MLVs, as expected, since the membranes are less hydrated [17,41,53].…”

Section: Mlbsmentioning

confidence: 54%

Section: Introductionmentioning

confidence: 99%

Direct comparison of elastic incoherent neutron scattering experiments with molecular dynamics simulations of DMPC phase transitions

et al. 2016

View full text Add to dashboard Cite

“…DB21MT-5, выделенных из бездны Челленджера, липиды мембран примерно на 70 % состояли из ненасыщенных жирных кислот . ВГД вы-зывает нарушение белок-липидных взаимодействий, необходимых для целостности биологических мембран и их нормального функционирования (Winter, Jeworrek, 2009). К числу важнейших причин гибели клеток при повышении давления относятся нарушение структуры и функций белков, а также диссоциация рибосом.…”

Section: Issn 1811-0932unclassified

High hydrostatic pressure influence on viability and mutagenesis of Salmonella Typhimurium

Karamova

Zelenikhin

Kiselev

et al. 2015

Ecological genetics

View full text Add to dashboard Cite

Background: pressure is a well-known physical environmental parameter. Nevertheless, the basic principles of microbial survival under high hydrostatic pressure (HHP), especially genetic response to pressure, are still poorly understood. The purpose of this study was to investigate the influence of HHP ranging from 50 to 800 MPa on viability and mutagenesis of Salmonella typhimurium. Materials and methods. The standard plate count method (counting the total number of colony forming units (CFUs) on the plate) and the propidium iodide (PI) flow cytometric assay were used to determine the bacterial viability after HHP treatment. Ability of HHP to induce gene mutations was examined by the Ames assay employing Salmonella typhimurium TA100 and TA98. Results. The results obtained showed that survival of S. typhimurium cells considerably decreased when bacteria were exposed to a pressure of 200 MPa and above. Herewith, the survival index calculated according to the total number of CFUs was up to six orders of magnitude lower than that obtained by the flow cytometric analysis under the same HHP. This fact can be explained by the entrance of the some part of bacterial population into the viable but nonculturable (VBNC) state. The pressure of 50 MPa was found to cause a 1.9-fold increase in the number of His+ revertants of S. typhimurium TA98 in Ames test. Conclusion. Our results demonstrate that HPP of 200 MPa and above significantly inhibits the viability of S. typhimurium cells as well as triggers the induction of VBNC state. The results of Ames test suggest that HHP of 50 MPa can induce gene mutations in bacterial cells. The possible mechanisms of HHP effects on cells viability as well as genetic response of bacteria under HHP are discussed.

show abstract

“…Thus, the microbial community structure and function response to hydrostatic pressure, ranging from 0.1 MPa to 1 MPa, is not clear. As a consequence, with increasing pressure the lipid bilayer loses fluidity and rapidly becomes impermeable to water and other molecules, and protein lipid interactions essential to the optimal function of the membrane are weakened [34]. Low temperature and high hydrostatic pressure have related and synergistic effects on biological membranes [34], reducing their fluidity by increasing the packing of fatty acyl chains.…”

mentioning

confidence: 99%

“…As a consequence, with increasing pressure the lipid bilayer loses fluidity and rapidly becomes impermeable to water and other molecules, and protein lipid interactions essential to the optimal function of the membrane are weakened [34]. Low temperature and high hydrostatic pressure have related and synergistic effects on biological membranes [34], reducing their fluidity by increasing the packing of fatty acyl chains. Our recent studies provided preliminary knowledge of hydrostatic pressure effects on the microbial community in three drinking water reservoir sediments [35].…”

mentioning

confidence: 99%