Effect of Pretreatments on the Enzymatic Hydrolysis of High-Yield Bamboo Chemo-Mechanical Pulp by Changing the Surface Lignin Content

Luo, Li; Yuan, Xiaoqi; Zhang, Sheng; Wang, Xuchong; Li, Mingfu; Wang, Shuangfei

doi:10.3390/polym13050787

Cited by 12 publications

(4 citation statements)

References 43 publications

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“…Many different pretreatments have been studied with bamboo substrates to date [ 9 ]. Among these pretreatment methods, dilute acid pretreatment appears to be most logical approach due to the low process costs and ease of operation [ 10 ]. However, the enzymatic digestibility of bamboo residues pretreated by acid is usually lower than 40%, which does not reach the level expected for industrialization.…”

Section: Introductionmentioning

confidence: 99%

Understanding the effects of different residual lignin fractions in acid-pretreated bamboo residues on its enzymatic digestibility

Lin

Yang

Zheng

et al. 2021

Biotechnol Biofuels

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Background During the dilute acid pretreatment process, the resulting pseudo-lignin and lignin droplets deposited on the surface of lignocellulose and inhibit the enzymatic digestibility of cellulose in lignocellulose. However, how these lignins interact with cellulase enzymes and then affect enzymatic hydrolysis is still unknown. In this work, different fractions of surface lignin (SL) obtained from dilute acid-pretreated bamboo residues (DAP-BR) were extracted by various organic reagents and the residual lignin in extracted DAP-BR was obtained by the milled wood lignin (MWL) method. All of the lignin fractions obtained from DAP-BR were used to investigate the mechanism for interaction between lignin and cellulase using surface plasmon resonance (SPR) technology to understand how they affect enzymatic hydrolysis Results The results showed that removing surface lignin significantly decreased the yield for enzymatic hydrolysis DAP-BR from 36.5% to 18.6%. The addition of MWL samples to Avicel inhibited its enzymatic hydrolysis, while different SL samples showed slight increases in enzymatic digestibility. Due to the higher molecular weight and hydrophobicity of MWL samples versus SL samples, a stronger affinity for MWL (KD = 6.8–24.7 nM) was found versus that of SL (KD = 39.4–52.6 nM) by SPR analysis. The affinity constants of all tested lignins exhibited good correlations (r > 0.6) with the effects on enzymatic digestibility of extracted DAP-BR and Avicel. Conclusions This work revealed that the surface lignin on DAP-BR is necessary for maintaining enzyme digestibility levels, and its removal has a negative impact on substrate digestibility.

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Section: Introductionmentioning

confidence: 99%

Understanding the effects of different residual lignin fractions in acid-pretreated bamboo residues on its enzymatic digestibility

Lin

Yang

Zheng

et al. 2021

Biotechnol Biofuels

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“…C1 mainly reflects the non-carbohydrate content, such as lignin and extracts, whereas C2 and C3 mainly reflect the content of carbohydrates. The theoretical O/C values of cellulose and lignin are 0.83 and 0.33, respectively [ 55 ].…”

Section: Resultsmentioning

confidence: 99%

Effects of Lipase and Xylanase Pretreatment on the Structure and Pulping Properties of Wheat Straw

et al. 2022

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Based on the reduction of environmental pollution, a biological enzyme assisted alkali-oxygen pulping method was explored to improve the delignification efficiency and fiber accessibility of wheat straw and improve the properties of wheat straw pulp. In this paper, lipase and xylanase were used to pretreat wheat straw and the effects of different enzyme types and enzyme dosage on the microstructure and pulp properties of wheat straw were investigated and experimented. The results showed that the lipase can remove fat and wax on the surface of wheat straw, while xylanase degraded the hemicellulose components, such as xylan, of wheat straw fiber, destroyed the structure of the lignin-carbohydrate complex, increasing lignin removal as a result and enhancing the impregnating, diffusion and penetration of alkali. Compared with wheat straw without enzyme pretreatment, the skeleton of wheat straw pretreated by enzyme became looser, the internal cavity appeared and the wall cavity became thin and transparent. The fines decreased obviously and the length of fibers increased. After combined pretreatment with lipase (15 U·g−1) and xylanase (15 U·g−1), the pulping performance of wheat straw was improved and the tensile index (97.37 N·m·g−1), brightness (40.9% ISO) and yield (58.10%) of the pulp increased by 12.9%, 19.9% and 9.9%, respectively. It can be seen that enzyme pretreatment is a green and effective approach to improving the alkali-oxygen pulping performance of wheat straw.

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“…Remarkably, the S/G ratio of untreated biomass did not exhibit any correlation with the enzymatic saccharification efficiency trend (Li et al, 2016;Yuan et al, 2021). The weak linear or polynomial relationship between the S/G ratio and enzymatic saccharification yield was evident in Figure 3(c).…”

Section: Structural Characterisation Of Biomasses By Fundamental Inst...mentioning

confidence: 91%

“…where O/Ccarbohydrate = 0.83 and O/Clignin = 0.33 (Luo et al, 2021) 2.9 Pyrolysis Gas Chromatography-Mass Spectrometry (Py-GCMS)…”

Section: X-ray Photoelectron Spectroscopy (Xps)mentioning

confidence: 99%

Integration of Multiple Fundamental Instrumental Analyses to Understand the Key Factors for Enhancing Enzymatic Saccharification of Oil Palm (Elaeis guineensis) Empty Fruit Bunch by Aqueous Pretreatments

Hemashini,

Lee,

Goh

et al. 2023

Preprint

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Simple aqueous pretreatments were performed on oil palm empty fruit bunch (OPEFB) to enhance its enzymatic digestibility by the cellulolytic enzyme. Autohydrolysis, acid and alkaline pretreatments were conducted at appropriate temperatures for 45 minutes with a solid: liquor ratio of 1:7. The refined pretreated OPEFBs were enzymatically saccharified at a fixed condition of 50°C, pH 4.8 for 48 hours. Besides chemical composition analysis, various changes in biomass during pretreatments were also evaluated using the fundamental instrumental analyses namely HPLC, FTIR, SEM, XRD, Py-GCMS and XPS. Autohydrolysed biomass achieved the maximum (> 99.9%) enzymatic saccharification/total glucose yield (TGY), attributed to 77% hemicellulose and 24% lignin removals, with only 16% cellulose loss. The crystallinity index was significantly correlated with TGY, but morphological analysis, S/G ratio of lignin, and O/C ratio were irrelevant. The key TGY-enhancing factors of pretreatment were unable to be identified by any single instrumental analysis. Determining the chemical composition of the biomass remains the primary fundamental analysis to understand the requirement for improving enzymatic yield.

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Effect of Pretreatments on the Enzymatic Hydrolysis of High-Yield Bamboo Chemo-Mechanical Pulp by Changing the Surface Lignin Content

Cited by 12 publications

References 43 publications

Understanding the effects of different residual lignin fractions in acid-pretreated bamboo residues on its enzymatic digestibility

Understanding the effects of different residual lignin fractions in acid-pretreated bamboo residues on its enzymatic digestibility

Effects of Lipase and Xylanase Pretreatment on the Structure and Pulping Properties of Wheat Straw

Integration of Multiple Fundamental Instrumental Analyses to Understand the Key Factors for Enhancing Enzymatic Saccharification of Oil Palm (Elaeis guineensis) Empty Fruit Bunch by Aqueous Pretreatments

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