Abstract:A field experiment was conducted on Sawargaon series of isohyperthermic family of Vertic haplustepts to study the effect of application of primary treated biomethanated spentwash (PBSW) through irrigation on soil properties, nutrient uptake and yield of soybean -wheat cropping sequence. The bulk density and hydraulic conductivity of soil were improved in 100% recommended dose (RD) of N through PBSW with and without P chemical fertilizer at soil depths of 0-15 and 15-30 cm. The lowest soil pH, calcium carbonate and highest electrical conductivity, organic carbon, available K, exchangeable sodium (Na), exchangeable sodium percentage (ESP), cation exchange capacity (CEC), sodium adsorption ratio (SAR) and pooled bacterial, fungal and actinomycetes populations in surface (0-15 cm) soil depth were observed in 100% RD of N through PBSW + with and without P chemical fertilizer at all three soil depths (0-15, 15-30 and 30-60 cm) over the other treatments. The highest pooled available soil P was recorded in 50% RD of N through PBSW application through irrigation and remaining N and P through chemical fertilizers in all three soil depths. The significantly highest total NPK uptake by cropping sequence and highest mean Benefit : Cost (B:C) ratio of soybean and wheat was recorded in 25 % RD of N through PBSW + remaining N and P through chemical fertilizers over the rest of the treatments. The 25 % N-recommended dose of soybean and wheat crop can be replaced by application of PBSW through irrigation in sequence without disturbing soils by salt load.