Effect of Probiotic Oral Administration on Skin Wound Healing in Rats

Abstract

Background:Manipulating intestinal microbiota with probiotics might stimulate skin response. Understanding all stages of the healing process, as well as the gut-skin-healing response can improve the skin healing process. Aim:To evaluate the effect of perioperative oral administration of probiotics on the healing of skin wounds in rats. Methods:Seventy-two Wistar male adult rats were weighed and divided into two groups with 36 each, one control group (supplemented with oral maltodextrin 250 mg/day) and one probiotic group (supplemented with Lactobacillus paracasei LPC-37, Bifidobacterium lactis HN0019, Lactobacillus rhamnosus HN001, Lactobacillus acidophilus NCFM® at a dose of 250 mg/day), both given orally daily for 15 days. The two groups were subsequently divided into three subgroups according to the moment of euthanasia: in the 3rd, 7th and 10th postoperative days. Results:There were no significant changes in weight in both groups. Wound contraction was faster in probiotic group when compared to the controls, resulting in smaller wound area in the 7th postoperative day. As for histological aspects, the overall H&E score was lower in the probiotic group. The probiotic group showed increased fibrosis from 3rd to the 7th postoperative day. The type I collagen production was higher in the probiotic group at the 10th postoperative day, and the type III collagen increased in the 7th. Conclusion:The perioperative use of orally administrated probiotic was associated with a faster reduction of the wound area in rats probably by reducing the inflammatory phase, accelerating the fibrosis process and the deposition of collagen.

Probiotic Activity of Staphylococcus epidermidis Induces Collagen Type I Production through FFaR2/p-ERK Signaling

“…causing the deterioration of connective tissue proteins such as collagen in the primary human skin dermal fibroblasts, leading to a loss of strength and flexibility in the skin [ 21 , 22 ]. Studies have evidenced that probiotics accelerate the fibrosis process, causing the deposition of collagen [ 8 ]. A mixture of S. epidermidis (10 7 CFU/mL) and CIN (2%) was topically applied to the dorsal skin of mouse for 1 week.…”

“…Metabolites from the human probiotic bacteria Staphylococcus epidermidis ( S. epidermidis ) fermentation of different carbon sources such as sugar or polymers have been found to protect skin from inflammation in response to stress [ 6 , 7 ]. The oral administration of probiotics could effectively heal wounds through the deposition of collagen on them [ 8 ]. Moreover, S. epidermidis has the ability to attach to materials coated with extracellular matrix proteins such as fibrinogen and collagen type I through GehD, an extracellular lipase [ 9 ].…”

The differences in the number of fibroblasts and blood vessels after the topical and systemic administration of Lactobacillus casei Shirota probiotics for the treatment of traumatic ulcers in Wistar rats (Rattus norvegicus)

“…L. casei Shirota is a Gram-positive bacterium whose cell wall contains an active molecular component of lipoteichoic acid (LTA), which is a pathogen-associated molecular pattern L. casei Shirota acts as an inducer, whereas LTA activates the oral mucosal epithelial cells through the TLR-2. This TLR activation induces the production of chemokines and cytokines, such as IL-8, which will stimulate the reepithelialization process, angiogenesis, and the formation of the extracellular matrix [11,19,20].…”

Fisiopatologia da cicatrização em pacientes portadores de diabetes mellitus/ Physiopathology of healing in patients with diabetes mellitus

“…Ambos constituem denominadores comuns por meio dos quais a hiperglicemia, a hipertensão arterial e a dislipidemia atuam na gênese dessas complicações. Deste modo, a persistência dessas alterações pode ser responsável pelo mecanismo de memória metabólica, relacionado ao surgimento de complicações 18 .…”