Objective: Our study examined the impact of propriety blends of <i>Bacillus</i> strain probiotics on the performance, egg quality, and faecal microflora of laying hens.Methods: A total of 183 Institut de selection Animale (ISA) brown laying hens aged 23 weeks with an average body weight of 1,894±72 g were randomly allocated into 3 groups as control (corn-soybean meal based diet, CON), 0.5 g/kg Enterosure probiotics (ET1, 3×10<sup>8</sup> colony-forming unit [CFU]/kg feed), and 5 g/kg Enterosure probiotics (ET2, 3×10<sup>9</sup> CFU/kg feed) administered in mashed form. At the completion of each phase hen day egg production (HDEP), average egg weight (AEW), feed intake, and faecal microbiota were evaluated.Results: HDEP and AEW were higher (p<0.05) in the ET2-supplemented diet in phase 3 (week 9 to 12) compared with CON. Egg mass (EM) was higher (p<0.05) in phase 2 at ET2, and also higher (p<0.05) in phase 3 at the ET1 and ET2-supplemented diets compared with CON. Feed conversion ratio was lower (p<0.05) in phase 3 at the ET1 and ET2-supplemented diets, with ET2 being the lowest compared with ET1 and CON. Yolk colour was higher (p<0.05) in the ET-supplemented diets at phase 3 compared with CON. <i>Bifidobacterium</i> spp. was higher (p<0.05) in the ET2- supplemented diet compared with CON in phase 2, while in In phase 3, <i>Lactobacillus</i> spp. and <i>Bifidobacterium</i> spp. were higher (p<0.05) in the ET-supplemented diets compared with CON. <i>Coliforms</i> were lower (p<0.05) in the ETsupplemented diets compared with CON in phase 3.Conclusion: The propriety blends of <i>Bacillus</i> strain probiotics supplements at 0.5 g/kg and 5 g/kg could improve the production and quality of eggs with more significance at 5 g/kg for HDEP, AEW and EM, which was achieved via the increase of beneficial microbiomes such as <i>Lactobacillus</i> spp., <i>Bifidobacterium</i> spp., and the decrease of pathogenic microbiomes like <i>Escherichia</i> coli and <i>Coliforms</i> which was speculated to improve gut barrier function and the reproductive hormone.