Effect of probiotic supplementation on growth performance, intestinal morphology, barrier integrity, and inflammatory response in broilers subjected to cyclic heat stress

Li, Qiufen; Wan, Gen; Peng, Chengying; Xu, Long; Yu, Yingmei; Li, Lin; Li, Guanhong

doi:10.1111/asj.13433

Cited by 28 publications

(23 citation statements)

References 76 publications

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“…Another remarkable change observed in this study was the CHE can simultaneously affect multiple organ indexes (liver: ALT, AST, ALP, γ-GT, TBIL, DBIL, and IBIL; kidney: CREA-S and UA; heat: CK, LDH, and CK-MB), showing heat stress could cause dysfunction in multiple organs. These results concur with those of previous studies, suggesting response to CHE involves multiple organs and systems ( 23 , 61 ). In the present study, we also examined molecular indices of oxidative stress; it showed that birds subjecting to chronic heat exposure increased oxidative stress, as shown by lower levels of T-AOC, SOD, and GSH-Px with higher MDA concentrations in serum.…”

Section: Discussionsupporting

confidence: 93%

“…Gut microbiota is now increasingly recognized as a hidden “metabolic organ” that plays a critical role in regulating the host metabolic homeostasis and energy balance, promoting the development and maturation of intestinal mucosa, maintaining intestinal barrier integrity in gut ( 22 , 23 ). A normal gut microbial community and complete mucosal morphology in birds are the bases for excluding pathogens and maintaining normal nutrient digestion and absorption ( 24 , 25 ).…”

Section: Introductionmentioning

confidence: 99%

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Gut Microbiota and Serum Metabolite Potential Interactions in Growing Layer Hens Exposed to High-Ambient Temperature

et al. 2022

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Emerging evidence has revealed the dysbiosis of gut microbiota contributes to development of metabolic diseases in animals. However, the potential interaction between gut microbiota and host metabolism in growing hens under metabolic disorder induced by chronic heat exposure (CHE) remains inconclusive. The aim of our study was to examine the potential association among the cecal microbiota community, physiological indicators, and serum metabolite profiles in CHE hens. One hundred and eighty Hy-Line Brown hens were randomly allocated into three groups: thermoneutral control (TN), heat stress (HS), and pair-fed (PF). The experiment lasted for 5 weeks, with the first 2 weeks serving as the adaptation period. Results showed that the expression level of heat shock protein 70 (HSP70) in both serum and cecal tissues was significantly increased in the HS group. Serum parameters analysis also revealed that CHE caused physiological function damage and metabolic disorders. These results suggest the experiment was successful, inducing chronic heat stress. 16S rRNA sequencing analysis showed that the CHE can clearly induce dysbiosis of the gut microbial community reflected in the increment of the F/B ratio. Besides, serum untargeted metabolomics revealed the relative concentrations of 40 metabolites were significantly altered in the HS group compared with the TN group. Pathway analysis showed that these metabolites were mainly involving the increased proteolysis rather than lipolysis, and this tendency could be a specific metabolic adaptation of the poultry. The pair-feed experiment showed that the above changes induced by CHE were partly independent from the reduction of feed intake. Mantel correlation analysis between gut microorganisms and physiological indicators showed that the phylum Firmicutes and Euryarchaeota have a potential interaction with a serum lipid parameter. Random forest analysis showed that both genus Faecalibacterium and Methanobrevibacter were important predictors of the CHE-induced lipid metabolism disorder. Taken together, our findings may contribute to a better understanding of the metabolic mechanisms underlying the energy metabolism imbalance caused by the CHE and provide novel insights into the host-microbes interactions and its effects on the metabolic adaptation of hens under chronic heat exposure.

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Section: Discussionsupporting

confidence: 93%

Section: Introductionmentioning

confidence: 99%

Gut Microbiota and Serum Metabolite Potential Interactions in Growing Layer Hens Exposed to High-Ambient Temperature

et al. 2022

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“…These results are compatible with the results of Al-Fataftah and Abdelqader ( 41 ) and Jahromi et al ( 42 ). On the other side, Li et al ( 43 ) reported that probiotic supplementation could improve the morphology of the intestine and barrier function and alleviate inflammatory response but has no effect on growth under heat exposure. Moreover, Sandikci et al ( 44 ) and Sohail et al ( 45 , 46 ) reported that probiotics have no effect on the growth of the broilers stressed with heat.…”

Section: Discussionmentioning

confidence: 99%

Impact of Dietary or Drinking Water Ruminococcus sp. Supplementation and/or Heat Stress on Growth, Histopathology, and Bursal Gene Expression of Broilers

et al. 2021

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This research was conducted to evaluate the impact of dietary or drinking water Ruminococcus sp. supplementation and/or heat stress (HS) on the growth, serum biochemistry, tissue antioxidant, phagocytic assay, histopathology, and bursa gene expression of broilers. Day-old broiler chicks were allotted into six groups according to HS and/or Ruminococcus with or without enzyme supplementation. The first group was the control one, with a formulated diet and normal environmental temperature but without any supplement. The second group fed on Ruminococcus-supplemented diet (1 kg/kg diet). The third group fed on a formulated diet without supplement, and Ruminococcus and digestive enzymes were given in drinking water (0.1 ml/L). The fourth one was the heat stress group, with a normal formulated diet. The fifth and the sixth groups served as second and third groups, respectively, but with heat stress. The results of this experiment indicated that thermal temperature negatively affected the parameters of growth performance, serum biochemical, tissue antioxidants, and phagocytic assay. Moreover, heat stress led to pathological lesions in the internal organs and affected the expression of some genes related to heat stress, including proapoptotic genes such as caspase8 and bax, inflammatory genes such as NF-κβ1, and heat shock protein such as HSP 70 in the bursal tissue. These bad effects and abnormalities were mitigated by Ruminococcus alone or with enzyme supplementation, which improved all the above-mentioned parameters.

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“…Heat stress on heat shock protein in the gutHeat-shock proteins (HSPs) are well-recognized as stress proteins and molecular chaperones that protect the internal cell environment by participating in protein folding, repair, localization and degradation influencing essential process such as cell signaling, transcription, protein synthesis, metabolism, and regulation of cellular redox conditions and thus regulating cell growth and survival[1,79,80]. The synthesis and expressions of HSP are stimulated under biotic and abiotic stress stimuli, particularly hyperthermia, oxidative stress, infections, diseases, and hypoxia, to protect cell proteins from oxidative stress and other harmful environmental conditions[79,80].…”

mentioning

confidence: 99%

Heat stress on microbiota composition, barrier integrity, and nutrient transport in gut, production performance, and its amelioration in farm animals

Patra

Kar

2021

J Anim Sci Technol

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Effect of probiotic supplementation on growth performance, intestinal morphology, barrier integrity, and inflammatory response in broilers subjected to cyclic heat stress

Cited by 28 publications

References 76 publications

Gut Microbiota and Serum Metabolite Potential Interactions in Growing Layer Hens Exposed to High-Ambient Temperature

Gut Microbiota and Serum Metabolite Potential Interactions in Growing Layer Hens Exposed to High-Ambient Temperature

Impact of Dietary or Drinking Water Ruminococcus sp. Supplementation and/or Heat Stress on Growth, Histopathology, and Bursal Gene Expression of Broilers

Heat stress on microbiota composition, barrier integrity, and nutrient transport in gut, production performance, and its amelioration in farm animals

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