Effect of Probiotics on the Traumatic Brain Injury

KARAKAYALI, Emine; KOCAMAZ, Erdoğan; ALPAY, Şüheda; Önal, Tuna; Öztatlıcı, Mustafa; DURUŞMA, Rabia; ÖZEL, Hasan Fehmi; METE, Mesut; KUTLU, Necip; Tuğlu, Mehmet İbrahim

doi:10.4274/forbes.galenos.2022.48658

Cited by 1 publication

(4 citation statements)

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“…Group 4, the PB treatment group (TBH+PB, n=10) rats were treated daily 500 μL of PB oral gavage for 7 days after trauma. [ 15 - 18 ]…”

Section: Methodsmentioning

confidence: 99%

“…L. rhamnosus HN001, and L. acidophilus NCFM ® which were used approximately at a dose of 2.5 × 109 CFU through oral gavage once a day as 500 μL for 7 days. [ 17 , 18 , 22 ]…”

Section: Methodsmentioning

confidence: 99%

“…Group 4, the PB treatment group (TBH+PB, n=10) rats were treated daily 500 µL of PB oral gavage for 7 days after trauma. [15][16][17][18] TBI Procedure Xylazine (10 mg/kg, Bayer, Istanbul, Türkiye) and ketamine hydrochloride (75 mg/kg, Parke Davis Istanbul, Türkiye) were used by intraperitoneal injection for anesthesia. TBI was provided by the drop weight technique which described and modified by the previous studies.…”

Section: Experimental Protocolmentioning

confidence: 99%

“…[16] EEG monitoring was performed before and after PB administration at 7 days after TBI, the rats were sacrificed at the end of the experiment and brain samples were used for histopathology. [15,[18][19][20][21]…”

Section: Experimental Protocolmentioning

confidence: 99%

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Histological and electroencephalographic demonstration of probiotic effect for reduce of oxidative stress and apoptosis in experimental traumatic brain injury

Karakayalı

2023

Ulus Travma Acil Cerrahi Derg

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BACKGROUND: The gut microbiota modulates nervous system function. In the literature, it has been shown that this modulation is used in many nervous system injuries through oxidative stress (OS) and apoptosis mechanisms. In this study, it was aimed to investigate the neuroprotective effects of probiotic (PB) treatment in a rat traumatic brain injury (TBI) model with histological and electroencephalographic (EEG) data. METHODS: Forty male Wistar albino rats were divided into four groups. Group 1 was the control group (CONTROL, n=10) and no trauma was applied. Group 2 was the trauma group with the weight-drop technique (TBH, n=10). Group 3 was the sham group (SHAM), (TBH+sterile saline [SS], n=10) rats were given 500 μL of SS per day by oral gavage. Group 4 was the PB treatment group, (TBH+PB, n=10) rats were treated daily for 7 days with 500 μL of PB oral gavage. Brain samples were collected 7 days after trauma. Histopathological evaluation of brain samples was done with HE. OS with Endothelial nitric oxide synthase, vascularization with Vascular Endothelial Growth Factor, gliosis with S100, and apoptosis with caspase 3 were evaluated immunohistochemically. Apoptotic index was determined with TUNEL. In addition, EEG and somatosensory evoked potential (SEP) recording findings were compared. RESULTS: It was determined by HE staining that there was a significant (P<0.001) damage in the TBI and sham groups compared to the control group. It was found that PB treatment provided a significant (P<0.01) improvement in the damage created. While OS (P<0.01), gliosis (P<0.01), and apoptosis (P<0.05) decreased with PB treatment, angiogenesis (P<0.01) increased. In support of these findings, in the software-mediated EEG and SUP examination; Delta wave power and theta/alpha ratio increased with TBI and decreased with PB treatment. CONCLUSION: The results showed that PB treatment provided a significant improvement in rats by reducing OS, apoptosis, and gliosis and increasing vascularity. To the best of our knowledge in the literature, it was shown for the 1st time that histological results for the treatment of PB were supported by software-mediated EEG and SEP analysis.

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“…Group 4, the PB treatment group (TBH+PB, n=10) rats were treated daily 500 μL of PB oral gavage for 7 days after trauma. [ 15 - 18 ]…”

Section: Methodsmentioning

confidence: 99%

“…L. rhamnosus HN001, and L. acidophilus NCFM ® which were used approximately at a dose of 2.5 × 109 CFU through oral gavage once a day as 500 μL for 7 days. [ 17 , 18 , 22 ]…”

Section: Methodsmentioning

confidence: 99%