Effect of Procedural-Related Variables on Melanocyte–Keratinocyte Suspension Transplantation in Nonsegmental Stable Vitiligo: A Clinical and Immunocytochemical Study

El-Zawahry, Bakr Mohamed; Esmat, Samia; Bassiouny, Dalia Ahmed; Zaki, Naglaa Sameh; Sobhi, Rehab Mohamed; Saleh, Marwah Adly; Abdel-Halim, Dalia; Hegazy, Rehab A.; Gawdat, Heba I.; Samir, N.; El‐Hawary, M. F. S.; Maadawi, Zeinab El; Gouda, Heba; Khorshied, Mervat M.

doi:10.1097/dss.0000000000000962

Cited by 20 publications

(41 citation statements)

References 24 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…4 El Zawahry found no significant difference in repigmentation comparing split skin with hair follicle grafts, despite lower cell counts in hair samples. 5 We found a lower rate of repigmentation and greater duration of disease in our study (14.17 ± 8.3 vs 4.27 ± 1.387 years in Singh study) which possibly contributes to this lower response rate.…”

contrasting

confidence: 51%

Efficacy of noncultured melanocyte‐keratinocyte cell suspension vs hair follicular cell suspension transfer in stable vitiligo: A randomized controlled trial

Moosavi

Mohammadi

Tavakkoli

et al. 2020

Dermatologic Therapy

View full text Add to dashboard Cite

Noncultured melanocyte-keratinocyte cell transplantation (NMKT) and noncultured autologous hair follicular extracted cell suspension (HFECS) are surgical methods for stable vitiligo. 1 Our study aimed to compare NMKT with HFECS in stable vitiligo, in a randomized controlled study. Fifteen eligible patients older than 12 years were recruited. Patients were randomized for treatment with NMKT, HFECS, or placebo. The majority of lesions (66.7%) were on face and hands. The mean duration of disease was 14.17 ± 8.3 years. We harvested hair follicles from occipital area and took split skin from anterolateral aspect of thigh. Suspension preparation was almost the same method used by Singh et al. 2 In placebo group, we only dermabraded manually the recipient site. Dressing was removed 1 week after surgery, and after 3 weeks, all patients underwent narrow band ultraviolet B radiation (NBUVB) twice weekly for 16 weeks. Patients were visited monthly up to 6 months and at 1 year. Recipient surface areas, 10 cm 2 in each patient, were measured by graph papers and color match were evaluated by two independent dermatologists as excellent (95-100%), good (65-94%), moderate (25-64%), and weak (0-24%). Re-pigmentation appeared between 3 and 6 weeks in all patients (except one in HFECS) and after 1 year was diffuse for all lesions except one in NMKT (Table 1). All treated lesions had excellent color match, except one hyperpigmentation in NMKT and one in placebo group (due to dermabrasion or NBUVB). Two donor sites also showed hyperpigmentation. No relapse was observed after 40% F

show abstract

contrasting

confidence: 51%

Efficacy of noncultured melanocyte‐keratinocyte cell suspension vs hair follicular cell suspension transfer in stable vitiligo: A randomized controlled trial

Moosavi

Mohammadi

Tavakkoli

et al. 2020

Dermatologic Therapy

View full text Add to dashboard Cite

show abstract

“… 13 A signifi-cant positive correlation between duration of stability and percentage repigmentation of the lesions was also found in a recent study. 29 …”

Section: Introductionmentioning

confidence: 99%

“… 6 No similar correlation was found by other authors. 29 The majority of authors used MKTP in treatment of lesions <100 cm 2 with a favorable outcome. 5 , 7 , 13 , 15 , 31 , 34 , 36 , 38 , 41 , 45 A few achieved favorable outcome for lesions up to 250 cm 2 .…”

Section: Introductionmentioning

confidence: 99%

Autologous non-cultured melanocyte–keratinocyte transplantation in the treatment of vitiligo: patient selection and perspectives

Bassiouny

Esmat

2018

CCID

Self Cite

View full text Add to dashboard Cite

Autologous non-cultured melanocyte–keratinocyte transplantation procedure (MKTP) is one of the simplest cellular grafting techniques. Various modifications were done over the years to make the technique easier and more economical which led to its great popularity among dermatologists. Proper patient selection and good technical skills are essential for achieving success with this technique. In this review, different patient-related and procedure-related factors that affect the outcome are discussed. This review may guide dermatologists to select suitable candidates, and explains what to expect in each case and indicates different techniques which can be used. The expected complications and stability of acquired pigmentation, which are an essential part of the pretreatment patient counseling, are also discussed.

show abstract

“…Studying and comparing each and every aspect of these modifications can lead to reaching the optimum technique. Several studies compared the effect of donor tissue used, 18,19 method of recipient site preparation, 18,20 and even dressing used postoperatively 20 . On reviewing literature, no comparative studies were performed to assess the impact of adding or omitting growth factors to the suspension medium.…”

Section: Discussionmentioning

confidence: 99%

Studying the effect of adding growth factors to the autologous melanocyte keratinocyte suspension in segmental vitiligo

Esmat

Bassiouny

Saleh

et al. 2020

Dermatologic Therapy

Self Cite

View full text Add to dashboard Cite

Addition of different growth factors to the medium used in autologous melanocyte‐keratinocyte transplantation procedure (MKTP) was reported in the literature. The aim of the current study was comparison of response to MKTP in segmental vitiligo (SV) with and without adding growth factors to the suspension medium. Eighteen cases with SV were randomly divided into two groups. In group A: Ham F12 medium was used for suspension and in group B: 5 ng/mL recombinant basic fibroblast growth factor (bFGF) and 25 mg/500 mL 3′5′ cyclic adenosine monophosphate (cAMP) were added to the medium. All cases received NB‐UVB twice weekly for 24 weeks. The area of vitiligo lesions was measured before and after therapy by point‐counting technique and complications were recorded. Excellent response (90%‐100% repigmentation) occurred in 5/9 cases (56%) in group A and 7/9 cases (78%) in group B (with growth factors). A significant decrease in the area of treated lesions before and after therapy was found in both groups A and B (P = .0012 and .0004, respectively), however, a higher percentage of reduction in area of vitiligo was seen in group B cases (70% in group A vs 90% in group B; P value: .028). Marginal halo was seen in five cases in group A and six in group B. In conclusion addition of bFGF and cAMP to MKTP medium improved the results of the procedure. It could be considered if economically feasible.

show abstract

Effect of Procedural-Related Variables on Melanocyte–Keratinocyte Suspension Transplantation in Nonsegmental Stable Vitiligo: A Clinical and Immunocytochemical Study

Abstract: Using different techniques in M-K susp produces comparable results. However, the distal fingers showed better results using combination of donor NCECS and recipient cryoblebs.

Cited by 20 publications

References 24 publications

Efficacy of noncultured melanocyte‐keratinocyte cell suspension vs hair follicular cell suspension transfer in stable vitiligo: A randomized controlled trial

Efficacy of noncultured melanocyte‐keratinocyte cell suspension vs hair follicular cell suspension transfer in stable vitiligo: A randomized controlled trial

Autologous non-cultured melanocyte–keratinocyte transplantation in the treatment of vitiligo: patient selection and perspectives

Studying the effect of adding growth factors to the autologous melanocyte keratinocyte suspension in segmental vitiligo

Contact Info

Product

Resources

About

Effect of Procedural-Related Variables on Melanocyte–Keratinocyte Suspension Transplantation in Nonsegmental Stable Vitiligo: A Clinical and Immunocytochemical Study

Abstract: Using different techniques in M-K susp produces comparable results. However, the distal fingers showed better results using combination of donor NCECS and recipient cryoblebs.

Cited by 20 publications

References 24 publications

Efficacy of noncultured melanocyte‐keratinocyte cell suspension vs hair follicular cell suspension transfer in stable vitiligo: A randomized controlled trial

Efficacy of noncultured melanocyte‐keratinocyte cell suspension vs hair follicular cell suspension transfer in stable vitiligo: A randomized controlled trial

Autologous non-cultured melanocyte&ndash;keratinocyte transplantation in the treatment of vitiligo: patient selection and perspectives

Studying the effect of adding growth factors to the autologous melanocyte keratinocyte suspension in segmental vitiligo

Contact Info

Product

Resources

About

Autologous non-cultured melanocyte–keratinocyte transplantation in the treatment of vitiligo: patient selection and perspectives