-Introduction. Many tropical fruits have great health potential due to the possible presence of bioactive phytochemicals. Natural food composition databases are very important sources of information relative to natural food biodiversity and nutritional properties. The objective of this study was to report for the first time on the native carotenoid composition of the fruit of four tropical species from Panama. Materials and methods. Fully mature fruit were collected in Panama and analyzed by HPLC-DAD-APCI-MS, after carotenoid extraction with acetone. Results and discussion. The carotenoid composition had never been previously reported for two of the investigated fruits, membrillo (Gustavia superba) and guanabana toreta (Annona purpurea). In membrillo, 5 different carotenoids were detected; the content of total carotenoids was 318.6 µg g −1 fresh weight (fw), with β-carotene showing a relative abundance of 75.3%. In guanabana toreta, 11 different carotenoids were detected; the total carotenoid content was 48.3 µg g −1 fw, with β-carotene and zeaxanthin showing a relative abundance of 26.9% and 27.5%, respectively. In jobo (Spondias mombin), 11 different carotenoids were detected; the total carotenoid content was 45.8 µg g −1 fw, with β-cryptoxanthin, α-carotene and β-carotene showing a relative abundance of 25.4%, 10.5% and 8.5%, respectively. In mamey (Mammea americana), 16 different carotenoids were detected; the total carotenoid content was 145.2 µg g −1 fw; in particular, 10 different violaxanthin fatty acid diesters, of both cis and trans isomers, were determined. Conclusion. The consumption of the fruit studied should be promoted both locally and abroad, because of the reported presence of these health-promoting phytochemicals.Keywords: Panama / membrillo (Gustavia superba) / guanabana toreta (Annona purpurea) / jobo (Spondias mombin) / mamey (Mammea americana) / carotenoids / HPLC-DAD-APCI-MS Résumé -Étude de la composition des fruits du membrillo (Gustavia superba), de l'atier (Annona purpurea), du prunier mombin (Spondias mombin) et de l'abricotier des Antilles (Mammea Americana). Introduction. Nombreux sont les fruits tropicaux à fort potentiel pour la santé en fonction de la présence de substances phytochimiques bioactives. Les bases de données sur la composition des aliments naturels sont des sources d'information de première importance pour caractériser la diversité alimentaire naturelle et ses propriétés nutritionnelles. L'objectif de cette étude est de décrire, pour la première fois, la composition en caroténoïdes natifs des fruits de quatre espèces tropicales natives au Panama. Matériels et méthodes. Les fruits récoltés à pleine maturité au Panama ont été analysés par méthode HPLC-DAD-APCI-MS après extraction des caroténoïdes à l'acétone. Résultats et discussion. La composition en caroténoïdes n'avait jamais été signalée auparavant pour deux espèces étudiées, le membrillo (Gustavia superba) et l'atier (Annona purpurea). Dans les fruits du membrillo, 5 caroténoïdes différents ont été détec...