Effect of proctodeal gland foam on sperm kinetics in Japanese quail (Coturnix japonica)

Farooq, Umar; Cho, S.; Rybnik-Trzaskowska, P.K.; Singh, Ramveer; Malecki, Irek

doi:10.1016/j.theriogenology.2014.09.006

Cited by 9 publications

(19 citation statements)

References 14 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…After the final collection, the foam was pooled from all males and 10% foam extract was prepared as described previously Farooq et al. ().…”

Section: Methodsmentioning

confidence: 99%

“…Semen dilution and storage, and the estimation of sperm concentration, were carried out as described previously by Farooq et al. (). Briefly, immediately after collection, semen was diluted with Nacl‐TES buffer in 1:2 ratio and stored for 10 min in water bath at 30°C.…”

Section: Methodsmentioning

confidence: 99%

“…The capture properties of the SCA ® system were adjusted as described previously by Farooq et al. (). For the initial recording, the default SCA ® “ chicken” VCL intervals were selected (i.e.…”

Section: Methodsmentioning

confidence: 99%

“…After each collection, foam was stored at −20°C in airtight containers. After the final collection, the foam was pooled from all males and 10% foam extract was prepared as described previously Farooq et al (2015).…”

Section: Preparation Of Proctodeal Gland Foam Extractmentioning

confidence: 99%

“…In this regard, in our previous study (i.e. Farooq, Cho, Rybnik‐Trzaskowska, Singh, & Malecki, ), we standardized the protocol for quail sperm analysis by CASA and this study is a next step in identification of cut‐off values that can be used by CASA for categorization of motile quail sperm into rapid medium and slow sperm categories (or subpopulations).…”

Section: Introductionmentioning

confidence: 99%

See 4 more Smart Citations

Appraisal and standardization of curvilinear velocity (VCL) cut‐off values forCASAanalysis of Japanese quail (Coturnix japonica) sperm

Farooq

Malecki

Mahmood

et al. 2017

Reprod Domestic Animals

View full text Add to dashboard Cite

One of the basic steps in objective analysis of sperm motility is the subdivision of a motile sperm population into slow, medium and rapid categories based on their velocity. However, for CASA analysis of quail sperm, the velocity values for categorization of slow, medium and rapid sperm have not yet been standardized. To identify the cut-off values of "velocity curvilinear" (VCL) for quail sperm categorization, we captured and analysed 22,300 tracks of quail sperm using SCA -CASA. The median and mean VCL values were 85 and 97 μm/s. To define the VCL cut-off values, we used two methods. In the first, we identified the upper (rapid sperm) and lower (slow sperm) cut-off values using: (i) median VCL ± 25% or ± 50% or ± 75% of median VCL value; (ii) first and third quartile values of VCL data (i.e. 25% cut-off setting); and (iii) 33% and 66% of VCL data. Among these settings, sperm categories and their corresponding motility characteristics recorded using the "25%" setting (i.e. slow ≤36 ≤ medium ≤154 ≤ rapid) were found the most realistic and coherent with male ranking by fertility. In the second method, we calculated heteroscedasticity in the total VCL data using PCA and the two-step clustering method. With this approach, the mean of the high and low clusters was 165 and 51 μm/s, respectively. Together, the mean from two methods suggested that, for SCA -CASA categorization of quail sperm, sperm should be classed as "rapid" at VCL ≥160 μm/s and "slow" at VCL ≤45 μm/s.

show abstract

“…After the final collection, the foam was pooled from all males and 10% foam extract was prepared as described previously Farooq et al. ().…”

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Preparation Of Proctodeal Gland Foam Extractmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%