Effect of progesterone and estradiol-17β on ovarian fluid secretion in the threespine stickleback, <i>Gasterosteus aculeatus</i> L., form <i>trachurus</i>

Lam, T.J.; Chan, Kenny Chi Wai; Hoar, William S.

doi:10.1139/z79-056

Cited by 9 publications

(13 citation statements)

References 10 publications

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“…Immersion in 17,2013-P did not produce a significant effect although a similar trend was seen. These findings support the suggestion of Lam et al (1979) that P plays a role in preventing overripening in fish. Lam et al (1979) found that P stimulated the secretion of ovarian fluid in the threespined stickleback.…”

Section: Discussionsupporting

confidence: 91%

“…These findings support the suggestion of Lam et al (1979) that P plays a role in preventing overripening in fish. Lam et al (1979) found that P stimulated the secretion of ovarian fluid in the threespined stickleback. On the basis of this and their earlier (Lam et al 1978) findings, they postulated that steroids secreted by POFs, particularly P, maintain the secretion of OF which in turn maintains the viability of ovulated eggs in the ovarian cavity.…”

Section: Discussionsupporting

confidence: 91%

“…An association between overripening and degenerative changes in postovulatory follicles was first noted by Lam et al (1978) in the threespined stickleback, Gasterosteus aculeatus. Lam et al (1978Lam et al ( , 1979 postulated that POFs while active secrete steroids which prevent the overripening of ovulated eggs in the ovarian cavity. That POFs are steroidogenic for a period of time has been shown in goldfish (Nagaham et al 1976) and other teleosts (Kagawa et al 1981;Nagahama et al 1982;Nagahama 1983).…”

Section: Discussionmentioning

confidence: 99%

“…However, the underlying causes are still not understood. Lam et al (1978Lam et al ( , 1979 have shown a relationship between overripening and the degeneration of postovulatory follicles (POFs) in the threespined stickleback (Gasterosteus acculeatus), and postulated that steroids (e.g., progesterone) secreted by the POFs stimulate the secretion of ovarian fluid which helps maintain the viability of ovulated eggs in the ovarian cavity. In goldfish, Nagahama et al (1976) have shown that POFs degenerate rapidly (within 30h) based on ultrastructural and histochemical evidence.…”

Section: Introductionmentioning

confidence: 99%

“…This bears a relationship to the time course of overripening obtained in this study. Therefore, to further examine the hypothesis of Lam et al (1978Lam et al ( , 1979, changes in the levels of serum and ovarian fluid steroid hormones during overripening, together with the histology of the POFs, were studied in goldfish. In vivo and in vitro experiments were also carried out to test the possible involvement of certain steroids in the process of overripening.…”

Section: Introductionmentioning

confidence: 99%

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Overripening of ovulated eggs in goldfish, Carassius auratus: II. Possible involvement of postovulatory follicles and steroids

Formacion

Venkatesh

Tan

et al. 1995

Fish Physiol Biochem

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Changes in steroid hormone levels in the serum and ovarian fluid during overripening were studied in goldfish. Ovulated eggs retained in the ovarian cavity became overripe at around 12h after ovulation based on loss of fertilizability, with advanced degeneration by 24h. Blood and ovarian fluid were taken at 0, 3, 6, 12, 18 and 24h after ovulation. Both serum and ovarian fluid progesterone (P) showed a highly significant decline by 18h with a further decline by 24h; P levels were higher in the ovarian fluid. Serum 17α,20β-dihydroxy-4-pregnen-3-one(17,20β-P) levels showed a progressive and more rapid decline, decreasing significantly by 12h with further decreases by 18h and 24h. Serum testosterone (T) levels increased significantly at 3h and remained high till 18h, thereafter they declined to the 0h level. No significant changes in estradiol-17β (E2) levels were observed in the serum, except for a significant difference between 6 and 24h. There were no significant changes in ovarian fluid E2, T or 17,20β-P levels.The postovulatory follicles (POFs) showed degenerative changes which corresponded to the decline in P and 17,20β-P. The hypothesis that overripening may be associated with declining levels of P or 17,20β-P was tested in vivo by immersing just ovulated females (0h) in P or 17,20β-P solutions for 12h, and in vitro by immersing just-ovulated eggs (0h) in ovarian fluid with an anti-serum against P (Anti-P). In the former, P at 0.05 ppm significantly enhanced the fertilization rate of ovulated eggs stripped from the females at 12h while 17,20β-P did not produce a significant effect at the tested concentrations (0.025 and 0.05 ppm) on the fertilization rate. In the latter, anti-P significantly lowered the fertilization rate of 0h ovulated eggs after 6h incubation. The evidence suggests a role of P in the maintenance of viability of ovulated eggs.

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Section: Discussionsupporting

confidence: 91%

Section: Discussionsupporting

confidence: 91%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Overripening of ovulated eggs in goldfish, Carassius auratus: II. Possible involvement of postovulatory follicles and steroids

Formacion

Venkatesh

Tan

et al. 1995

Fish Physiol Biochem

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Ovarian fluid and its impacts on spermatozoa performance in fish: A review

et al. 2019

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Factors such as gamete quality can profoundly affect fertility, but the spawning microenvironment that surrounds the spermatozoa and eggs during gamete contact has largely been neglected. In fishes, understanding these gametic interactions is crucial because each female creates a unique spawning environment by simultaneously expelling her distinct ovarian fluid (OF) along with an egg batch. In turn, OF has been shown to influence spermatozoa performance traits by modifying spermatozoa behaviors and fertilization outcomes. Here, we shed light on these gametic interactions by overviewing literature on OF and how it impacts spermatozoa performance traits. Fish OF is clear or has slight coloration and can constitute ≤10-30% of egg mass. Viscosity of the OF is ~2-to 3-fold higher than water and its pH ranges 6.2 to 8.8. Osmolality of the OF is lower in freshwater (190-322 mOsmol/kg) than marine species (289-514 mOsmol/kg). Na + (98.3-213.7 mmol/L) and Cl -(89.8-172.7 mmol/L) are predominant ions in OF, while K + (1.7-19.3 mmol/L), Mg 2+ (0.4-8.1 mmol/L), and Ca 2+ (0.5-9.7 mmol/L) ions are detected at lower concentrations. Protein levels can be high in OF and exhibit intra-and inter-species variation (54-826 mg/100mL). Fish OF also contains a series of organic components and substances that enhance and/or attract sperm towards the vicinity of an egg. OF can also differentially impact sperm based on genetic relatedness of mates, male phenotype (i.e. alternative reproductive tactics), or geographic origin. To conclude, when testing further reproductive paradigms, we suggest a shift from classic spermatozoa activation medium (water only) to more natural spawning media, which encompass OF-spermatozoa interactions.

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Ovarian early postovulatory development and oestrogen production in rainbow trout (Salmo gairdneri R.) from a spring-spawning strain

Mones

Fostier

Cauty

et al. 1989

General and Comparative Endocrinology

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Ovarian early postovulatory development and oestrogen production were studied in rainbow trout from a spring-spawning strain, 5 to 50 days postovulation (PO). Early postovulatory development was assessed by the ovarian development index, corresponding to the relative volume occupied in the ovary by oocytes in an advanced stage of differentiation (containing cortical alveoli). This index was shown to be related to the maximal oocyte diameter and to the time since ovulation, as well as to metabolic criteria. In contrast, gonadosomatic index was not correlated with any of these, confirming its unreliability for assessing the gonadal development, at least at this stage. Size of follicles and presence of vitellus (lipoprotein) in the oocytes of several animals indicate that ovarian development was more advanced in our females than in other studies. It is suggested that young follicles start developing before ovulation of the preceding batch of oocytes. Oestradiol (E2) plasma levels were low but detectable, and showed, in some females, peak values (max 5.5 ng/ml) within the first month PO. Besides, in vitro metabolism was studied using two isotopes of androstenedione as precursors. No sharp discrepancies were observed between the two types of incubation. Conjugation of the precursor or its metabolites was high but decreased with time postovulation. This suggests that it proceeds from postovulatory follicles. E2 production potential was demonstrated from the shortest time postovulation. Moreover, it increased with ovarian development, suggesting that E2 is synthesized in developing follicles.

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Effect of progesterone and estradiol-17β on ovarian fluid secretion in the threespine stickleback, Gasterosteus aculeatus L., form trachurus

Cited by 9 publications

References 10 publications

Overripening of ovulated eggs in goldfish, Carassius auratus: II. Possible involvement of postovulatory follicles and steroids

Overripening of ovulated eggs in goldfish, Carassius auratus: II. Possible involvement of postovulatory follicles and steroids

Ovarian fluid and its impacts on spermatozoa performance in fish: A review

Ovarian early postovulatory development and oestrogen production in rainbow trout (Salmo gairdneri R.) from a spring-spawning strain

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