Effect of progesterone on nitric oxide/cyclic guanosine monophosphate signaling and contraction in gastric smooth muscle cells

Al‐Shboul, Othman; Mustafa, Ayman G.; Omar, Amal Abu; Al‐Dwairi, Ahmed; Alqudah, Mohammad; Nazzal, Mona S.; Alfaqih, Mahmoud A.; Al-Hader, Rami

doi:10.3892/br.2018.1161

Cited by 7 publications

(7 citation statements)

References 43 publications

(68 reference statements)

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“…We also sought to explore the change in estrogen effect on NO/cGMP pathway in the stomach muscle cells during pregnancy. This research in pregnant GMSC is in continuation of our previously reported effect of estrogen and progesterone in nonpregnant female [30, 31]. Our findings may be of considerable importance in understanding the cause of the various pregnancy-associated GI tract motility disorders and would further pave the way for understanding the ER-mediated smooth muscle contraction-relaxation pathways and thereby establishing novel therapeutic approaches for treatment of GI disorders.…”

Section: Introductionsupporting

confidence: 82%

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Changes in Gastric Smooth Muscle Cell Contraction during Pregnancy: Effect of Estrogen

Al‐Shboul

Al-Rshoud

Al‐Dwairi

et al. 2019

Journal of Pregnancy

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It is well known that pregnancy is associated with frequent gastrointestinal (GI) disorders and symptoms. Moreover, previous reports have shown that estrogen, which changes in levels during pregnancy, participates in the regulation of GI motility and is involved in the pathogenesis of various functional disorders in the stomach. The aim of the current study was to explore the changes in the expression of estrogen receptors (ERs) and examine the effect of estrogen on nitric oxide- (NO-) cyclic guanosine monophosphate (cGMP) pathway and thus relaxation in gastric smooth muscle cells (GSMC) during pregnancy. Single GSMC from early-pregnant and late-pregnant Sprague-Dawley rats were used. Protein and mRNA expression levels of ERs were measured via specifically designed enzyme-linked immunosorbent assay (ELISA) and polymerase chain reaction (PCR), respectively. NO and cGMP levels were measured via specifically designed ELISA kits. Effect of estrogen on acetylcholine- (ACh-) induced contraction of single GSMC was measured via scanning micrometry in the presence or absence of the NO synthase inhibitor,N-nitro-L-arginine (L-NNA), or guanylyl cyclase inhibitor, 1H-[1,2,4]oxadiazolo[4,3,-a]quinoxalin-1-one (ODQ). Estrogen increased both NO and cGMP levels and their levels were greater in early compared to late pregnancy. Expression of ERs was greater in early compared to late pregnancy. ACh induced greater contraction of GSMC in late pregnancy compared to early pregnancy. Estrogen inhibited ACh-induced contraction in both periods of pregnancy. Importantly, pretreatment of GSMC with either L-NNA or ODQ abolished estrogen inhibitory action on muscle contraction. In conclusion, GSMC contractile behavior undergoes drastic changes in response to estrogen during pregnancy and this might explain some of the pregnancy-associated gastric disorders.

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Section: Introductionsupporting

confidence: 82%

“…In addition, estrogen induces relaxation of vascular smooth muscle by a process that involves activation of NO-cGMP pathway [29]. Most importantly, we recently reported estrogen- and progesterone-induced relaxation in stomach smooth muscle cells via activation of the NO-cGMP pathway [30, 31].…”

Section: Introductionmentioning

confidence: 99%

Changes in Gastric Smooth Muscle Cell Contraction during Pregnancy: Effect of Estrogen

Al‐Shboul

Al-Rshoud

Al‐Dwairi

et al. 2019

Journal of Pregnancy

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“…Most studies have shown that progesterone inhibits intestinal smooth muscle cells mainly by increasing nitric oxide synthesis to induce smooth muscle relaxation. In addition, progesterone can inhibit signaling pathways that mediate contraction, such as Rho kinase [ 42 , 43 ]. Hermoso and Villalo found that interstitial ICC not only expresses connexin Cx43, but also that the level of sex hormones affect the expression and function of connexin [ 44 , 45 ].…”

Section: Discussionmentioning

confidence: 99%

Regulation of PDGFRα+ cells and ICC in progesterone-mediated slow colon transit in pregnant mice

Lu,

Luo,

Wang

et al. 2024

Heliyon

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“…Therefore, an increase in NO level can cause the smooth muscles in the eye to relax [47]. Nitric oxide is synthesized from L-arginine by NO synthase and is known to relax vascular smooth muscle through the elevation of cGMP that, in turn, mediates a relaxation response [38,[48][49][50][51]. NO synthase has been found in ciliary muscle and other ocular tissues [52].…”

Section: Discussionmentioning

confidence: 99%

“…Targets of CC. The Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform (TCMSP, https://old.tcmsp-e.com/tcmsp.php) was utilized to screen active ingredients of CC by using "Juhua," "Juemingzi," "Gouqizi," "Huangjing," "Nvzhenzi," and "Mohanlian" as keywords, with the criteria OB ≥ 30% and DL ≥ 0:18 [38].…”

Section: Screening the Effective Components And Potentialmentioning

confidence: 99%

Network Pharmacology, Molecular Docking, and Molecular Dynamic-Based Investigation on the Mechanism of Compound Chrysanthemum in the Treatment of Asthenopia

Qiu

Zheng

Zhou

et al. 2022

Computational and Mathematical Methods in Medicine

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As a clinical empirical prescription for ophthalmology, compound chrysanthemum has been used gradually and has a good effect on eye fatigue. However, the detailed mechanisms of antiasthenopia have not been studied. In order to clarify the mechanisms of the compound chrysanthemum in the treatment of asthenopia, network pharmacology was combined with experimental study in this paper. A total of 593 genes and 39 active chemicals were identified, and both were considered to be essential to the advancement of asthenopia research. The results of the molecular docking analysis demonstrated a certain affinity between PRKACA, PRKCA, PRKCB, and their related compounds; molecular dynamic simulations assessed the stability of these receptors and ligands. The effects of compound chrysanthemum extract on ciliary muscle were studied in vitro and in vivo. By using the MTT assay, compound chrysanthemum extracts (50, 100, 200, 400, and 800 g·mL-1) showed no effect on the proliferation of rCSMCs for 24 and 48 hours. It raised nitric oxide and decreased Ca2+ in ciliary muscle cells isolated from the eyeballs of rats. Besides, compound chrysanthemum extract had a direct relaxing effect on the isolated gastric smooth muscle of rats by reducing the contractile tension. Furthermore, in vivo experiment results showed that, compared to the incandescent lamp-irradiated rats (model group), SD rats treated with compound chrysanthemum extracts (660 mg·kg-1 and 1320 mg·kg-1, orally) displayed considerably retracted pupils and increased NO content. It is also found that compound chrysanthemum extract can downregulate the mRNA expression of PKA and PKC in the calcium signaling pathway. Overall, our results suggested that compound chrysanthemum extract may lessen visual fatigue through multiple components, multiple targets, and multiple pathways.

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Effect of progesterone on nitric oxide/cyclic guanosine monophosphate signaling and contraction in gastric smooth muscle cells

Cited by 7 publications

References 43 publications

Changes in Gastric Smooth Muscle Cell Contraction during Pregnancy: Effect of Estrogen

Changes in Gastric Smooth Muscle Cell Contraction during Pregnancy: Effect of Estrogen

Regulation of PDGFRα+ cells and ICC in progesterone-mediated slow colon transit in pregnant mice

Network Pharmacology, Molecular Docking, and Molecular Dynamic-Based Investigation on the Mechanism of Compound Chrysanthemum in the Treatment of Asthenopia

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