Effect of Propionibacterium freudenreichii on Salmonella multiplication, motility, and association with avian epithelial cells

Nair, Divek V. T.; Johny, Anup Kollanoor

doi:10.3382/ps/pew367

Cited by 16 publications

(19 citation statements)

References 72 publications

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“…Heidelberg. A study conducted by Nair and Kollanoor-Johny [ 162 ] revealed that probiotic P. freudenreichii isolated from fermented dairy products was effective against major virulence factors of multidrug-resistant S. Heidelberg. Compared to the antibiotic-resistant parent strains, probiotic-treated S. Heidelberg showed reduced multiplication, motility, and adhesion on intestinal epithelial cells.…”

Section: Ongoing Studies With Alternative Interventions Against Mumentioning

confidence: 99%

Antibiotic-Resistant Salmonella in the Food Supply and the Potential Role of Antibiotic Alternatives for Control

Nair

Venkitanarayanan

Johny

2018

Foods

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210

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Salmonella enterica is one of the most ubiquitous enteropathogenic bacterial species on earth, and comprises more than 2500 serovars. Widely known for causing non-typhoidal foodborne infections (95%), and enteric (typhoid) fever in humans, Salmonella colonizes almost all warm- and cold-blooded animals, in addition to its extra-animal environmental strongholds. The last few decades have witnessed the emergence of highly virulent and antibiotic-resistant Salmonella, causing greater morbidity and mortality in humans. The emergence of several Salmonella serotypes resistant to multiple antibiotics in food animals underscores a significant food safety hazard. In this review, we discuss the various antibiotic-resistant Salmonella serotypes in food animals and the food supply, factors that contributed to their emergence, their antibiotic resistance mechanisms, the public health implications of their spread through the food supply, and the potential antibiotic alternatives for controlling them.

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Section: Ongoing Studies With Alternative Interventions Against Mumentioning

confidence: 99%

Antibiotic-Resistant Salmonella in the Food Supply and the Potential Role of Antibiotic Alternatives for Control

Nair

Venkitanarayanan

Johny

2018

Foods

Self Cite

210

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“…The pellet was resuspended in 10 ml of PBS, and the bacterial populations in the culture were confirmed by plating 0.1 ml of appropriate dilutions on MRS plates. Viable PF and PS populations were determined after incubating the plates at 41°C (turkey body temperature) for 48 h ( Nair and Kollanoor-Johny, 2017a ).…”

Section: Methodsmentioning

confidence: 99%

“…A US poultry outbreak isolate of MDR SH was used in the study (GT2011; Nair and Kollanoor-Johny, 2017a , b ; Nair et al, 2018 ). Glycerol stocks of SH stored at −80°C were used for the preparation of working cultures.…”

Section: Methodsmentioning

confidence: 99%

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Characterizing the Antimicrobial Function of a Dairy-Originated Probiotic, Propionibacterium freudenreichii, Against Multidrug-Resistant Salmonella enterica Serovar Heidelberg in Turkey Poults

Nair

Johny

2018

Front. Microbiol.

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Antimicrobial potential of a dairy-origin probiotic bacteria, Propionibacterium freudenreichii, against multidrug-resistant Salmonella Heidelberg (SH) in turkey poults was determined in the current study. Employing in vitro experiments, two strains (subsp.) of P. freudenreichii: P. freudenreichii freudenreichii B3523 (PF) and P. freudenreichii shermanii B4327 (PS) were tested for their ability to resist low pH (2.5) and bile salts (0.3%). In addition, the ability of the strains to adhere to and invade avian epithelial cells was determined after exposure to Propionibacterium strains followed by SH challenge. Moreover, the antibacterial activity of the strains’ cell-free culture supernatants (CFCSs) were tested against three major foodborne pathogens, including SH. Furthermore, the susceptibility of the strains to common antibiotics used for human therapy was determined. The hemolytic properties of the strains were determined in comparison to Streptococcus pyogenes, a known hemolysis-causing pathogen. Appropriate controls were kept in all studies. Using two in vivo experiments, PF was tested against SH colonization of poult ceca and dissemination to liver and spleen. The four treatment groups were: negative control, PF control (PFC), SH control (SC), and a test group (PFS; PF + SH). The poults in the PFC and PFS groups were inoculated with 1010 CFU ml−1 PF on day 1 through crop gavage and subsequently supplemented through drinking water. On day 7, SC and PFS groups were challenged with SH at 106 CFU ml−1, and after 7 days, cecum, liver, and spleen were collected for determining surviving SH populations. Results indicated that both PF and PS resisted pH = 2.5 and 0.3% bile salts with surviving populations comparable to the control and adhered well onto the avian epithelial cell lines. The strains were susceptible to antibiotics and did not invade the epithelial cells or exhibit hemolytic properties. The CFCSs were highly bactericidal against all tested pathogens. In turkey poults, PF significantly reduced cecal colonization of SH and the dissemination of the pathogen to the liver, compared to the SH challenge controls (P < 0.05). Results revealed that PF, a non-host gastrointestinal tract-derived probiotic, could be an antibiotic alternative to prevent the early colonization of SH in poults, improving the preharvest safety of turkeys.

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“…Propionibacteria adhesion to intestinal cells leads to exclusion of invasive pathogenic bacteria by competitive adhesion or co-aggregation mechanisms. This concerns pathogens such as Escherichia coli , Pseudomonas aeruginosa , S taphylococcus aureus and Salmonella enterica [41,42,43,44]. Adhesion mechanisms remain poorly understood, but some preliminary experiments point out a role of surface proteins and teichoic acid in adhesion mechanisms [37].…”

Section: Gut Persistence Of Dairy Propionibacteriamentioning

confidence: 99%

Dairy Propionibacteria: Versatile Probiotics

2017

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Dairy propionibacteria are used as cheese ripening starters, as biopreservative and as beneficial additives, in the food industry. The main species, Propionibacterium freudenreichii, is known as GRAS (Generally Recognized As Safe, USA, FDA). In addition to another dairy species, Propionibacterium acidipropionici, they are included in QPS (Qualified Presumption of Safety) list. Additional to their well-known technological application, dairy propionibacteria increasingly attract attention for their promising probiotic properties. The purpose of this review is to summarize the probiotic characteristics of dairy propionibacteria reported by the updated literature. Indeed, they meet the selection criteria for probiotic bacteria, such as the ability to endure digestive stressing conditions and to adhere to intestinal epithelial cells. This is a prerequisite to bacterial persistence within the gut. The reported beneficial effects are ranked according to property’s type: microbiota modulation, immunomodulation, and cancer modulation. The proposed molecular mechanisms are discussed. Dairy propionibacteria are described as producers of nutraceuticals and beneficial metabolites that are responsible for their versatile probiotic attributes include short chain fatty acids (SCFAs), conjugated fatty acids, surface proteins, and 1,4-dihydroxy-2-naphtoic acid (DHNA). These metabolites possess beneficial properties and their production depends on the strain and on the growth medium. The choice of the fermented food matrix may thus determine the probiotic properties of the ingested product. This review approaches dairy propionibacteria, with an interest in both technological abilities and probiotic attributes.

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Effect of Propionibacterium freudenreichii on Salmonella multiplication, motility, and association with avian epithelial cells

Cited by 16 publications

References 72 publications

Antibiotic-Resistant Salmonella in the Food Supply and the Potential Role of Antibiotic Alternatives for Control

Antibiotic-Resistant Salmonella in the Food Supply and the Potential Role of Antibiotic Alternatives for Control

Characterizing the Antimicrobial Function of a Dairy-Originated Probiotic, Propionibacterium freudenreichii, Against Multidrug-Resistant Salmonella enterica Serovar Heidelberg in Turkey Poults

Dairy Propionibacteria: Versatile Probiotics

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