Effect of Propofol on microRNA Expression Profile in Adipocyte-Derived Adult Stem Cells

Abstract

MicroRNA (miRNA) pathways have been implicated in stem cell regulation. This study investigated the molecular effects of propofol on adipocyte stem cells (ASCs) by analyzing RNA expression arrays. Human ASCs were isolated by use of a liposuction procedure. ASCs were treated with saline, 50 µM propofol, or 100 µM propofol in culture media for 3 hours. After the isolation of total RNA, the expression of 76 miRNAs was evaluated with peptide nucleic acid-miRNA array analysis through denaturation and hybridization processes. Treatment with 50 µM propofol resulted in significant down-regulation of expression of 18 miRNAs and upregulation of expression of 25 miRNAs; 100 µM propofol resulted in significant downregulation of expression of 14 miRNAs and upregulation of expression of 29 miRNAs. The lowest expression was seen for miR-204, which was 0.07-fold with 50 µM propofol and 0.18-fold with 100 µM propofol. The highest expression was seen for miR-208b, which was 11.23-fold with 50 µM propofol and 11.20-fold with 100 µM propofol. Expression patterns of miRNAs were not significantly different between 50 µM and 100 µM propofol treatment. The results of this study suggest that propofol is involved in altering the miRNA expression level in human ASCs. Additional research is necessary to establish the functional effect of miRNA alteration by propofol.

Impact of Hyperpolarization-activated, Cyclic Nucleotide-gated Cation Channel Type 2 for the Xenon-mediated Anesthetic Effect

“…[131][132][133] other studies demonstrated effects of propofol on human stem cells, leading to alterations in mirNA expression levels. 15,134 in addition, some recent studies also indicate functional relevance for certain mirNAs in the pathogenesis or protection from neurotoxicity, respectively. Moreover, some mirNAs have already been identified to present potential targets that could be modulated to enhance the mirNA-mediated neuroprotective effects.…”

MicroRNA‑433 inhibits cell growth and induces apoptosis in human cervical cancer through PI3K/AKT signaling by targeting FAK

“…This process forms a continuous lesion, which frequently occurs over 10 years (14). Early identification and timely treatment can substantially improve the survival rate of patients with cervical squamous carcinoma (15). Therefore, investigating the mechanisms underlying the invasion and metastasis of cervical cancer cell is of the highest priority (16).…”

Emerging Roles for MicroRNAs in Perioperative Medicine

“…131–133 Other studies demonstrated effects of propofol on human stem cells, leading to alterations in miRNA expression levels. 15,134 In addition, some recent studies also indicate functional relevance for certain miRNAs in the pathogenesis or protection from neurotoxicity, respectively. Moreover, some miRNAs have already been identified to present potential targets that could be modulated to enhance the miRNA-mediated neuroprotective effects.…”