Effect of Prostaglandin E1 on Adjuvant Arthritis

Zurier, Robert B.; Quagliata, Franco

doi:10.1038/234304a0

Cited by 243 publications

(87 citation statements)

References 8 publications

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“…Our data do not permit such an extrapolation in the absence of additional studies, although evidence for a possible antiarthritic effect of PGEs may be found in the reported ability of these prostanoids to suppress adjuvant arthritis in rats and collagen-induced arthritis in mice (60,61). Similarly, the clinical relevance of ERK inhibition by salicylates or other agents remains to be determined.…”

Section: Discussionmentioning

confidence: 62%

Cyclooxygenase-2-Derived E Prostaglandins Down-Regulate Matrix Metalloproteinase-1 Expression in Fibroblast-Like Synoviocytes via Inhibition of Extracellular Signal-Regulated Kinase Activation

Pillinger

Rosenthal

Tolani

et al. 2003

The Journal of Immunology

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We examined the regulation of matrix metalloproteinase (MMP) production by mitogen-activated protein kinases and cyclooxygenases (COXs) in fibroblast-like synoviocytes (FLSCs). IL-1β and TNF-α stimulated FLSC extracellular signal-regulated kinase (ERK) activation as well as MMP-1 and -13 release. Pharmacologic inhibitors of ERK inhibited MMP-1, but not MMP-13 expression. Whereas millimolar salicylates inhibited both ERK and MMP-1, nonsalicylate COX and selective COX-2 inhibitors enhanced stimulated MMP-1 release. Addition of exogenous PGE1 or PGE2 inhibited MMP-1, reversed the effects of COX inhibitors, and inhibited ERK activation, suggesting that COX-2 activity tonically inhibits MMP-1 production via ERK inhibition by E PGs. Exposure of FLSCs to nonselective COX and selective COX-2 inhibitors in the absence of stimulation resulted in up-regulation of MMP-1 expression in an ERK-dependent manner. Moreover, COX inhibition sufficient to reduce PGE levels increased ERK activity. Our data indicate that: 1) ERK activation mediates MMP-1 but not MMP-13 release from FLSCs, 2) COX-2-derived E PGs inhibit MMP-1 release from FLSCs via inhibition of ERK, and 3) COX inhibitors, by attenuating PGE inhibition of ERK, enhance the release of MMP-1 by FLSC.

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Section: Discussionmentioning

confidence: 62%

Cyclooxygenase-2-Derived E Prostaglandins Down-Regulate Matrix Metalloproteinase-1 Expression in Fibroblast-Like Synoviocytes via Inhibition of Extracellular Signal-Regulated Kinase Activation

Pillinger

Rosenthal

Tolani

et al. 2003

The Journal of Immunology

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“…PGE, injections usually caused reduction in spontaneous activity for I hour and transient passage of soft stools. These adverse effects were not as profound as those observed in rats treated with PGE, (6). Results in 9 mice injected with saline daily were no different from results in 10 mice injected twice daily.…”

Section: Methodsmentioning

confidence: 59%

Prostaglandin E₁ treatment of NZB/NZW mice

Zurier

Sayadoff

Torrey

et al. 1977

Arthritis & Rheumatism

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133

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NZB/NZW F, hybrid mice were treated with pharmacologic doses of prostaglandin El (PGE,) (200 pg subcutaneously either once or twice daily) from 6 through 52 weeks of age. PGE,-treated mice were protected against development of anemia, clinical nephritis, and death. At 52 weeks, 18 of 19 treated mice were alive, whereas only 2 of 19 untreated control mice were alive. None of the 10 mice treated with PGE, twice daily exhibited significant (>2+) proteinuria at 1 year of age. PGE, treatment did not prevent development of antibodies to nuclear antigens. The data also suggest that survival of NZB/NZW mice is prolonged when treatment with PGE, is begun at 24 weeks, an age at which mice already show evidence of nephritis. Thus all 6 mice treated with PGE, (200 pg sc twice daily) from 24 weeks were alive a t 52 weeks, whereas only 2 of 6 untreated control mice were alive. The mechanisms whereby PGE, treatment influences the course of disease in NZB/NZW mice are not known.

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“…Paradoxically, PG have also anti-in¯ammatory and immunosuppressive effects [8]. In vitro, treatment of T lymphocytes with PG inhibits their activation and proliferation [9±11].…”

Section: Introductionmentioning

confidence: 99%

Cyclooxygenase-1 and -2 are expressed by human T cells

Pablos¹,

Santiago²,

Carreira³

et al. 1999

Clinical and Experimental Immunology

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SUMMARYIn vitro, prostaglandins (PG) have strong inhibitory effects on T cell activation and proliferation and inhibitors of PG synthesis (NSAID) increase proliferation and activation of T cells. Although most studies have failed to demonstrate cyclooxygenase (COX) activity in lymphocytes, there is contradictory evidence on the synthesis of different PG. We have studied by reverse transcriptase-polymerase chain reaction (RT-PCR) and Western blot the expression of COX-1 and -2 mRNA and protein in resting and activated peripheral blood or Jurkat T cells. Cells were activated by T cell receptor triggering with OKT3 antibodies and activation con®rmed by¯ow cytometric analysis of surface CD69. COX enzymatic activity was measured by determination of arachidonic acid (AA)-induced PG synthesis. Both peripheral blood and Jurkat T cells expressed COX-1 and -2 mRNA and protein. COX-1 was constitutively expressed and did not change after OKT3 stimulation. COX-2 was inducible upon OKT3-induced activation. In spite of the presence of COX mRNA and immunoreactive protein, AA-induced PG synthesis was not detected at the EIA detection (pM) level. The potential role of cyclooxygenases in T cells deserves further study, since no PG of the studied series seem to be synthesized by T cells.

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Effect of Prostaglandin E1 on Adjuvant Arthritis

Cited by 243 publications

References 8 publications

Cyclooxygenase-2-Derived E Prostaglandins Down-Regulate Matrix Metalloproteinase-1 Expression in Fibroblast-Like Synoviocytes via Inhibition of Extracellular Signal-Regulated Kinase Activation

Cyclooxygenase-2-Derived E Prostaglandins Down-Regulate Matrix Metalloproteinase-1 Expression in Fibroblast-Like Synoviocytes via Inhibition of Extracellular Signal-Regulated Kinase Activation

Prostaglandin E₁ treatment of NZB/NZW mice

Cyclooxygenase-1 and -2 are expressed by human T cells

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Effect of Prostaglandin E1 on Adjuvant Arthritis

Cited by 243 publications

References 8 publications

Cyclooxygenase-2-Derived E Prostaglandins Down-Regulate Matrix Metalloproteinase-1 Expression in Fibroblast-Like Synoviocytes via Inhibition of Extracellular Signal-Regulated Kinase Activation

Cyclooxygenase-2-Derived E Prostaglandins Down-Regulate Matrix Metalloproteinase-1 Expression in Fibroblast-Like Synoviocytes via Inhibition of Extracellular Signal-Regulated Kinase Activation

Prostaglandin E1 treatment of NZB/NZW mice

Cyclooxygenase-1 and -2 are expressed by human T cells

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Prostaglandin E₁ treatment of NZB/NZW mice