Effect of prostaglandins on luteal function during early pregnancy in pigs

Więsak, Teresa; Hunter, M. G.; Foxcroft, G. R.

doi:10.1530/jrf.0.0950831

Cited by 21 publications

(11 citation statements)

References 41 publications

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“…Our results showed that PGE 2 as well as PGF 2a increased secretion of P 4 by luteal slices collected from early pregnant pigs. Similar results were observed in previous studies on porcine luteal cells (Wiesak et al 1992). Moreover, PGE 2 increased P 4 synthesis and cAMP production via both PTGER2 and PTGER4.…”

Section: Rescue Of Luteal Function During Early Pregnancysupporting

confidence: 91%

Steroid hormones, prostanoids, and angiogenic systems during rescue of the corpus luteum in pigs

Przygrodzka¹,

Kaczmarek²,

Kaczyński³

et al. 2016

Reproduction

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In order to characterize the transition of the corpora lutea (CL) from acquisition of luteolytic sensitivity to rescue of luteal function: i) the expression of 38 factors associated with steroids, prostanoids, and angiogenic systems and ii) concentrations of the main hormones responsible for maintenance of CL function in cyclic and pregnant pigs were examined. Additionally, the effect of prostaglandin (PG) E 2 and F 2a on luteal function during the estrous cycle and pregnancy was evaluated in vitro. Significantly up-regulated gene expression was revealed in CL collected on day 14 of the estrous cycle (CYP19A1, ESR2, PTGS2, HIF1A, and EDN1) and on days 12-14 of pregnancy (SCARB1, PGRMC1, STAR, HSD3B1, NR5A1, PTGFR, PTGER4, and VEGFA). Elevated concentrations of estradiol-17b and PGE 2 occurred in CL on days 12 and 14 of pregnancy respectively, while an increased intraluteal PGF 2a content was noted on day 14 of the estrous cycle. Both PGs increased the synthesis of progesterone by cultured luteal slices obtained on day 14 of pregnancy, in contrast to the action of PGF 2a on the corresponding day of the estrous cycle. PGE 2 stimulated cAMP production via PTGER2 and PTGER4, while PGF 2a elevated the content of CREB in cultured luteal slices from CL of pregnant pigs. In silico analysis showed that infiltration of lymphocytes and apoptosis of microvascular endothelium were activated in CL on day 12 of the estrous cycle vs pregnancy. Summarizing, an abundance of E 2 and PGE 2 during pregnancy regulates specific pathways responsible for steroidogenesis, the prostanoid signaling system and angiogenesis during rescue from luteolysis in porcine CL.

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Section: Rescue Of Luteal Function During Early Pregnancysupporting

confidence: 91%

Steroid hormones, prostanoids, and angiogenic systems during rescue of the corpus luteum in pigs

Przygrodzka¹,

Kaczmarek²,

Kaczyński³

et al. 2016

Reproduction

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“…The 30% increase of PGE 2 was non-significant in the present experiment whereas a significant increase of 300% was observed by . It is now recognized that under the influence of a conceptus signal (probably a burst of estrogen synthesis from blastocyst cells), uterine secretion of PGs changes from an endocrine direction, which exerts its luteolytic effect on corpora lutea, to an exocrine direction, which allows for continuation of the gestation (Bazer et al 1992;Wiesak et al 1992;Roberts et al 1993;Bazer et al 1994). In the present experiment, the in vitro synthesis of PGE 2 and PGF 2α by the endometrial tissue suggests that, in gilts, the efficiency of uterus tissue for PG secretion is limited and does not require large amounts of folic acid to reach its maximum potential.…”

Section: Uterine and Conceptus Measurementsmentioning

confidence: 99%

Pre- and post-mating dietary supplements of folic acid and uterine secretory activity in gilts

Duquette¹,

Matte²,

Farmer³

et al. 1997

Can. J. Anim. Sci.

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-P. 1997. Pre-and post-mating dietary supplements of folic acid and uterine secretory activity in gilts. Can. J. Anim. Sci. 77: 415-420. The present study was carried out to determine the effects of pre-and (or) post-mating dietary supplements of folic acid on uterine secretions and secretory activity on day 12 of gestation. Crossbred gilts were assigned randomly to three treatments: SS) a dietary supplement of 15 mg of folic acid kg -1 of diet from the estrus before mating (approximately day -21) until day 12 of gestation (n = 9), 0S) the same folic acid supplement from mating (day 0) to day 12 of gestation (n = 10), and 00) no supplement of folic acid (n = 10). At slaughter (day 12 of gestation), one uterine horn was flushed with 20 mL of PBS to collect embryos and uterine flushings, while samples of uterine tissue were collected from the other horn. Supplementary folic acid (0S and SS) increased total folates in uterine flushings (P ≤ 0.05) as well as concentrations of folates in the endometrium (P ≤ 0.0004) and in the whole uterine tissue (endometrium + myometrium: P ≤ 0.0001). Total amounts of prostaglandin (PG)E 2 and PGF 2α in uterine flushings were not affected (P ≥ 0.8) by any treatment but estradiol-17β was numerically 40% lower (P ≥ 0.12) in uterine flushings of 0S and SS sows. Samples of endometrium (15-17 mg) were cultured for 2 to 7 h. Concentrations of PGE 2 and PGF 2α in the culture medium increased with the duration of incubation (P ≤ 0.0001) but there was no treatment effect (P ≥ 0.4). The inconsistency between the folic acid response seen in the present study and in previous results using multiparous sows suggests that the impact of this vitamin on sow reproduction might be linked to the parity (and/or prolificacy) of the animal. . L'objectif de la présente étude visait à déterminer l'effet de compléments alimentaires d'acide folique avant et (ou) après la saillie sur certains aspects de la composition des sécrétions utérines et sur la fonction sécrétrice de l'utérus à 12 jours de gestation chez des truies primipares. Vingt neuf cochettes ont été assignées à trois traitements: SS) un complément alimentaire d'acide folique de 15 ppm à partir de l'oestrus précédant la saillie fécondante (environ -21 jours) jusqu'à 12 jours de gestation (n = 9), 0S) le même complément offert à partir de la saillie jusqu'à 12 jours de gestation (n = 10) et 00) aucun complément d'acide folique dans l'aliment. L'une des deux cornes utérines a été utilisée pour la récolte des embryons et du liquide utérin en vue des dosages hormonaux et métaboliques alors que l'autre corne a servi à la collecte du tissu endométrial. Les concentrations en folates de l'endomètre + myomètre et le contenu total en folates des sécrétions utérines étaient plus élévées (P ≤ 0,05) chez les truies 0S et SS comparativement aux truies 00. Les quantités totales de prostaglandine (PG)E 2 et de PGF 2α dans le liquide utérin n'étaient pas affectées (P ≥ 0,8) par les traitements mais celles de l'oestradiol-17β étaient numériquement 40% plus basse (P...

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“…No início da gestação o crescimento folicular está restrito aos folículos de até seis milímetros de diâmetro. Eles entram em atresia devido ao baixo estímulo gonadotrófico, porém, por volta do 12º dia de gestação verifica-se a presença de folículos capazes de responder ao estímulo de LH (Wiesak et al, 1992). Assim, protocolos de aplicação de gonadotrofinas em suínos deveriam considerar a aplicação de duas doses, a primeira visando o desenvolvimento dos folículos pequenos, e a segunda a formação de corpos lúteos acessórios.…”

Section: Resultsunclassified

Efeito da aplicação de hCG ou GnRH sobre a concentração sérica de progesterona e eficiência reprodutiva em porcas

Carvalho¹,

Filho²,

Silva³

et al. 2003

Arq. Bras. Med. Vet. Zootec.

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Efeito da aplicação de hCG ou GnRH sobre a concentração sérica de progesterona e eficiência reprodutiva em porcas RESUMOAvaliou-se o efeito da aplicação de diferentes hormônios no quinto dia após a primeira inseminação sobre a concentração sérica de progesterona e sobre as características reprodutivas, em 103 porcas entre o terceirro e sexto parto. As matrizes foram divididas em: grupo-controle (n=35), não tratado, grupo GnRH (n=34), animais submetidos à aplicação intramuscular (IM) de 50mcg de um análogo-GnRH no quinto dia após a primeira inseminação, e grupo hCG (n=34), animais submetidos à aplicação IM de 500UI de hCG no quinto dia após a primeira inseminação. A aplicação dos hormônios não influenciou as características reprodutivas taxa de parto, número total de nascidos, número de nascidos vivos e peso da leitegada (P>0,05). Cinco animais de cada grupo foram submetidos a coletas de sangue da veia cava nos dias 3, 5, 8, 12, 21 e 28 após a primeira inseminação para avaliação da concentração sérica de progesterona (ng/ml), utilizando a técnica de radioimunoensaio. Não houve diferença significativa quanto à concentração sérica de progesterona entre os grupos.Palavras-chave: suínos, GnRH, hCG, progesterona, gestação 3, 5, 8, 12, 21, 28, after the first insemination, to evaluate serum progesterone concentrations (ng/ml). Serum progesterone concentrations were not affected (P>0.05) by hormone treatments. ABSTRACT Two different hormones were administered on the fifth day after the first insemination to evaluate their influence on serum progesterone concentrations and on reproductive efficiency, in multiparous sows

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Effect of prostaglandins on luteal function during early pregnancy in pigs

Cited by 21 publications

References 41 publications

Steroid hormones, prostanoids, and angiogenic systems during rescue of the corpus luteum in pigs

Steroid hormones, prostanoids, and angiogenic systems during rescue of the corpus luteum in pigs

Pre- and post-mating dietary supplements of folic acid and uterine secretory activity in gilts

Efeito da aplicação de hCG ou GnRH sobre a concentração sérica de progesterona e eficiência reprodutiva em porcas

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