Effect of protein during hydrothermal carbonization of brewer’s spent grain

Arauzo, Pablo J.; Du, Lin; Olszewski, Maciej P.; Zavala, M.F. Meza; Alhnidi, Muhammad‐Jamal; Kruse, Andrea

doi:10.1016/j.biortech.2019.122117

Cited by 37 publications

(22 citation statements)

References 44 publications

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“…On the other hand, isoelectric point was at 2.92. This value was lower than 3.3 and 3.8 reported by Arauzo et al 18 and Connolly et al 19 for BSG proteins, respectively.…”

Section: Resultscontrasting

confidence: 63%

Physical, structural and antioxidant properties of brewer's spent grain protein films

et al. 2020

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BACKGROUND: The development of brewer's spent grain protein (BSG-PC) films with potential active packaging properties was investigated. Films were prepared by casting protein dispersions at different pH values (2, 8, 11), plasticizers [polyethylene glycol (PEG) or glycerol] and levels (0-0.25 g g −1) of PEG. Mechanical, water-barrier and solubility, optical, antioxidant (reducing power, ABTS •+ and lipidic radical scavenging), and antimicrobial properties of films were determined. Also, the structural characteristics of films were evaluated by attenuated total reflectance-Fourier transform infrared spectroscopy. RESULTS: Only films prepared at pH 2 and plasticized by PEG were homogeneous in appearance and could be manipulated; thus, different levels of PEG were studied at this pH. Higher PEG concentrations increased water solubility, water vapor permeability and elongation at break, and decreased tensile strength and elastic modulus. PEG increased ⊍-helix structure only when 0.10 g PEG g-1 BSG-PC was used. This could be related to the better mechanical properties of F 0.10 films (higher tensile strength, and elastic modulus) with respect to the other films. Antioxidant activity depended on PEG concentration, whereas no antimicrobial properties against Bacillus cereus, Salmonella newport and Penicillium corylophylum were detected. CONCLUSION: The formulations with 0.10 and 0.15 g PEG g −1 BSG-PC appear to be the most promising, balancing mechanical, water-barrier properties and the antioxidant capacity of these films. Moreover, BSG proteins could be a cheap alternative for the preparation of biodegradable films, which are capable of being used as active food packaging.

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“…On the other hand, isoelectric point was at 2.92. This value was lower than 3.3 and 3.8 reported by Arauzo et al 18 and Connolly et al 19 for BSG proteins, respectively.…”

Section: Resultscontrasting

confidence: 63%

Physical, structural and antioxidant properties of brewer's spent grain protein films

et al. 2020

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“…During aqueous extraction, the osmosis-caused diffusion in water through cell or cellulose structure was not strong enough for protein molecule permeation [37]. Strong alkaline conditions can partly remove the cellulosic structures with soluble protein dissolving in water [27,28,38]. Alkaline treatment showed the highest protein extraction yield for BSG (21.4 wt.%) and AP (55.5 wt.%), which is comparable with previous studies [39,40].…”

Section: Extraction Methods Comparisonsupporting

confidence: 68%

“…BSG_pH and AP_H 2 O covered the full range of molecular weights that were marked. As was discussed in the previous study, the BSG protein concentrates recovered with pH shifting method is promising as functioning agents in the food industry [28]. The BSG protein fractions of molecular weights between 14.5 and 50 KD perform better given emulsifying and foaming [39,53].…”

Section: Molecular Weightmentioning

confidence: 79%

“…Although alkaline treatment led to high protein extraction yield, unextracted protein remained in the residue. The previous study showed that the protein residue of BSG, after alkaline treatment, contains 3.63% of N [28]. Further subcritical water treatment might further destroy the lignocellulosic structure of the residues, resulting in a promoted protein extraction efficiency.…”

Section: Protein Extraction Yield and Puritymentioning

confidence: 99%

“…Through disulfide cross-linking breakage, the extractability of the protein is enhanced. It consists of the disruption of cell walls to extract protein easier and this extractability is influenced by certain extraction conditions such as biomass type, pH, temperature, and extraction time [10,27,28]. Subcritical water treatment is emerging as a "green" extraction method avoiding the introduction of chemicals.…”

Section: Introductionmentioning

confidence: 99%

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Towards the Properties of Different Biomass-Derived Proteins via Various Extraction Methods

Arauzo

Zavala

et al. 2020

Molecules

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This study selected three representative protein-rich biomass—brewer’s spent grain (BSG), pasture grass (PG), and cyanobacteria (Arthrospira platensis; AP) for protein extraction with different extraction methods (alkaline treatment, aqueous extraction, and subcritical water extraction). The yield, purity, molecular weight, oil–water interfacial tension, and thermal stability of the obtained proteins derived from different biomass and extraction methods were comprehensively characterized and compared. In the view of protein yield and purity, alkaline treatment was found optimal for BSG (21.4 and 60.2 wt.%, respectively) and AP (55.5 and 68.8 wt.%, respectively). With the decreased oil–water interfacial tension, the proteins from all biomass showed the potential to be emulsifier. BSG and AP protein obtained with chemical treatment presented excellent thermal stability. As a novel method, subcritical water extraction is promising in recovering protein from all three biomass with the comparable yield and purity as alkaline treatment. Furthermore, the hydrolyzed protein with lower molecular weight by subcritical water could promote its functions of foaming and emulsifying.

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