Effect of protein malnutrition on the neurobehavioural toxicity of styrene in young rats

Khanna, Vinay K.; Husain, Raushan; Seth, Prahlad K.

doi:10.1002/jat.2550140506

Cited by 34 publications

(12 citation statements)

References 28 publications

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“…Radioligand binding assay of DA-D2 receptors and expression of DAR-D2 receptor gene in corpus striatum. Assay of dopamine (DA)-D2 receptors in the corpus striatum was carried out by the radioligand receptor binding following the procedure as described by Khanna et al (1994). Briefly, to prepare the crude synaptic membrane, the corpus striatum was homogenized in 19 volumes of Tris-HCl buffer (5 mM, pH 7.4) and the homogenate was centrifuged at 40,000 × g (15 min, 4 • C).…”

Section: Animals and Treatmentmentioning

confidence: 99%

Early life arsenic exposure and brain dopaminergic alterations in rats

Chandravanshi

Shukla

Sultana

et al. 2014

Intl J of Devlp Neuroscience

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Recently, we found that early life exposure to arsenic at low doses resulted to cause brain cholinergic deficits and exhibited a trend of recovery on withdrawal of arsenic exposure. In continuation to this, the present study has been carried out to assess the impact of low level arsenic exposure on brain dopaminergic system and associated behavior in developing rats and investigate if neurobehavioral changes are recovered or persistent. Early life exposure (PD22-PD59) to arsenic (2 or 4 mg/kg body weight, p.o.) in rats resulted to increase the motor activity on PD60, compared to controls. The hyperactivity in arsenic exposed rats was found to be linked with increase in the binding of DA-D2 receptors (38%, 56%), mRNA expression of DAR-D2 receptor gene (68%, 97%) and expression of tyrosine hydroxylase protein (1.93, 2.73-fold) in the corpus striatum as compared to controls on PD60. Exposure to arsenic enhanced generation of ROS (47%, 84%) and was associated with decrease in the mitochondrial membrane potential (13.3%, 15.33%), activity of mitochondrial complexes and increased oxidative stress. Disruption in the expression of pro-apoptotic, anti-apoptotic and stress marker proteins was also distinct in the corpus striatum of arsenic exposed rats. The severity of changes in the behavioral and neurochemical endpoints were found to persist in rats exposed to arsenic at high dose and exhibited a trend of recovery at low dose on withdrawal of arsenic exposure on PD90. Early life arsenic exposure appears to be critical and vulnerable as development of dopamine receptors continues during this period.

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Section: Animals and Treatmentmentioning

confidence: 99%

Early life arsenic exposure and brain dopaminergic alterations in rats

Chandravanshi

Shukla

Sultana

et al. 2014

Intl J of Devlp Neuroscience

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“…11) The prenatal and early postnatal PM also causes various abnormal behaviors, such as increased exploratory behavior, 12,13) reduced anxiety, 13,14) decreased social interaction, 15) increased depressive behavior, 16) increased aggressive behavior, 17,18) prepulse inhibition deficits 19) and impairment of memory-related behavior. [20][21][22] In contrast, there are remarkably few reports concerning postweaning PM effects on the CNS.…”

Section: )mentioning

confidence: 99%

Executive Functions of Postweaning Protein Malnutrition in Mice

Sato

Nakagawasai

Tan-No

et al. 2011

Biological & Pharmaceutical Bulletin

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An inadequate supply of nutrition is one of the main nongenetic factors affecting brain development.1) Liu et al. 2) demonstrated that children with malnutrition signs at three years of age tend to have low IQ, excessive motor activity and antisocial behavior in adulthood. In the framework of such nutritional inadequacy, early protein malnutrition (PM) adversely affects central nervous system (CNS) maturation, resulting in long-lasting, or even permanent, deleterious effects. 3,4)Malnutrition is a common problem worldwide and occurs in both developing and developed countries. In developing countries malnutrition is associated with poverty or socioeconomic problems, whereas in developed countries, the weight of the newborn is influenced by the mothers diet 5) and the weight at birth affects subsequent development. 6) Also, in developed countries, severe malnutrition is commonly found in patients with eating disorders which frequently occurs during adolescence leading to interruptions of somatic and psychological development. The use of common weight control techniques by healthy weight adolescents can produce chronic undernutrition. 7)In rats, there are two principal types of PM experimental models: prenatal (i.e., gestation period) and early postnatal (i.e., lactation period) malnutrition. There are many studies focused on body and brain morphological affects of these PM models, such as body and brain weight, 3) hippocampal effects, 8,9) span of basilar dendrities 10) and neocortical effects.11) The prenatal and early postnatal PM also causes various abnormal behaviors, such as increased exploratory behavior, 12,13) reduced anxiety, 13,14) decreased social interaction, 15) increased depressive behavior, 16) increased aggressive behavior, 17,18) prepulse inhibition deficits 19) and impairment of memory-related behavior. [20][21][22] In contrast, there are remarkably few reports concerning postweaning PM effects on the CNS. Lukoyanov and Andrade 23) found that eight months of PM in adult rats induced a marked loss of neurons and synapses in the hippocampal formation and that the morphological alterations led to noticeable impairments in openfield behavior and spatial learning in the water maze. Thus, even if PM begins in adult animals, abnormal behaviors are induced which take a long time to express.The human brain develops rapidly in the last third of pregnancy and the first two years of life. Brain weight increases with age and achieves adult weight between six and 14 years of age. By two years of age the brain is approximately 80% of the adult weight and at one time it was believed that, to a large extent, brain development had ended. More recently it has become apparent that brain development continues through adolescence and even as adults the brain can adapt to changing circumstances. Adolescence is a particularly important time for brain development as more adult ways of thinking emerge: abstract thinking, deductive reasoning and the ability to solve problems. 24) There is increasing evidence that diet...

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“…Assay of dopamine receptors in the corpus striatum was carried out by the method of Khanna et al (1994). Briefly, crude synaptic membranes were prepared by homogenizing the tissue in 19 volumes of 0.32 M sucrose followed by centrifugation (50 000Âg, 10 min, 4°C).…”

Section: Dopamine Receptor Assaymentioning

confidence: 99%

Protective effect of Acorus calamus against acrylamide induced neurotoxicity

Shukla

Khanna

Ali

et al. 2002

Phytotherapy Research

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103

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Exposure of rats to acrylamide (ACR) caused hind limb paralysis in 58% of the animals on day 10 and decreased behavioural parameters, namely distance travelled, ambulatory time, stereotypic time and basal stereotypic movements compared with the control group. These rats also had a decrease in the reduced glutathione (GSH) content and glutathione-S-transferase (GST) activity in the corpus striatum and an increase in striatal dopamine receptors, as evident by an increase in the binding of 3H-spiperone to striatal membranes. Treatment with the ethanol:water (1:1) extract of the rhizomes of Acorus calamus (AC-002) increased the GSH content and GST activity in the corpus striatum while insignificant changes were observed in other parameters. Rats treated with ACR and AC-002 in combination had a lower incidence of paralysis (18%) compared with those treated with ACR alone on day 10 of the experiment. The rats also showed a partial recovery in other behavioural parameters. The levels of GSH content and GST activity increased in the corpus striatum, while the dopamine receptors decreased compared with the ACR treated rats. The results suggest that the neurobehavioural changes produced by ACR may be prevented following treatment with Acorus calamus rhizomes.

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Effect of protein malnutrition on the neurobehavioural toxicity of styrene in young rats

Cited by 34 publications

References 28 publications

Early life arsenic exposure and brain dopaminergic alterations in rats

Early life arsenic exposure and brain dopaminergic alterations in rats

Executive Functions of Postweaning Protein Malnutrition in Mice

Protective effect of Acorus calamus against acrylamide induced neurotoxicity

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