Effect of Proteolytic Agents on Microleakage of Etch and Rinse Adhesive Systems

Kasraei, Shahin; Yarmohammadi, Ebrahim; Farhadian, Maryam; Malek, Mona

doi:10.20396/bjos.v16i0.8651051

Cited by 5 publications

(7 citation statements)

References 29 publications

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“…Our results are consistent with previous studies and may be explained by the depletion of collagen from the acid-etched dentin caused by the action of bromelain and papain. 16,23 However, the SEM observations in this study were inconsistent with the results of the SBS test, which showed that the same 3 groups -those deproteinized with 10% papain, 15% papain and 6% bromelain -had higher SBS values than the other groups, although no statistically significant difference was found between any of the treatment groups and the control group. This could be attributed to other factors, such as the type of adhesive system used.…”

Section: Discussioncontrasting

confidence: 74%

“…In agreement with the current findings, Kasraei et al reported that the treatment of the acid-etched dentin surface with 5% bromelain before the application of the adhesive had no significant effect on marginal microleakage of Class V composite restorations. 23 The SEM micrographs of the resin-dentin interface after the application of bromelain showed that the hybrid layer and resin tags were thick, and the resin tags were conical in shape in the bromelain-treated group. Additionally, spherical residues were observed on resin tags due to the minor infiltration of resin into accessory canals in the dentin.…”

Section: Discussionmentioning

confidence: 94%

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Effects of different concentrations of bromelain and papain enzymes on shear bond strength of composite resin to deep dentin using an etch-and-rinse adhesive system

Sharafeddin,

jowkar,

safari

2024

Dent. Med. Probl.

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Background. The dentin substrate can be modified by proteolytic agents, which may affect the bonding strength of adhesive systems to the treated dentin surface. Papain, a cysteine protease enzyme with antibacterial and anti-inflammatory properties, can be used for deproteinization of dentin. An alternative deproteinizing enzyme is bromelain.Objectives. This study aimed to evaluate the impact of deproteinization on the shear bond strength (SBS) of composite resin to deep dentin using different concentrations of bromelain and papain.Material and methods. Sixty upper premolars were extracted and randomly divided into 5 groups (n = 12 per group). In all groups, the dentin surface was etched with 37% phosphoric acid. Group 1 did not receive any enzyme treatment, group 2 was treated with a 10% papain solution, group 3 was treated with a 15% papain solution, group 4 was treated with a 6% bromelain solution, and group 5 was treated with a 10% bromelain solution. After applying an etch-and-rinse adhesive system, the specimens were restored with composite resin and the SBS was measured.Results. Statistically significant differences were found between groups 2 and 3 (10% papain and 15% papain, p = 0.004), groups 2 and 4 (10% papain and 6% bromelain, p = 0.017), groups 4 and 5 (6% bromelain and 10% bromelain, p = 0.021), and groups 3 and 5 (15% papain and 10% bromelain, p = 0.005).Conclusions. Deproteinization with papain and bromelain at different concentrations after acid etching did not affect the SBS of composite resin to deep dentin when using an etch-and-rinse adhesive system. However, the group deproteinized with 15% papain demonstrated a higher SBS than the group deproteinized with 10% papain, and the group deproteinized with 6% bromelain showed a higher SBS compared to the group deproteinized with 10% bromelain.

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Section: Discussioncontrasting

confidence: 74%

Section: Discussionmentioning

confidence: 94%

Effects of different concentrations of bromelain and papain enzymes on shear bond strength of composite resin to deep dentin using an etch-and-rinse adhesive system

Sharafeddin,

jowkar,

safari

2024

Dent. Med. Probl.

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“…Dentin conditioning with acidic material may demineralize more of the dentine surface, so the applied adhesive resin monomers can infiltrate and produce a weaker hybrid layer at this important interface(Andermatt & Özcan, 2021). With the purpose of eliminating the influence of the organic matrix on the adhesion of the composites to the dentin, various substances with deproteinizing characteristic were suggested, like papain and bromelain enzymes(Sharafeddin & Safari, 2019).In the present study, no significant difference was observed in the microleakage between phosphoric acid 37% and those treated with bromelain enzyme 10% Kasraei et al (2017). indicated that there was no significant difference in microleakage between the bromelaintreated and acid-etched surfaces Dayem and Tameesh (2013).…”

mentioning

confidence: 44%

“…In the present study, no significant difference was observed in the microleakage between phosphoric acid 37% and those treated with bromelain enzyme 10%. Kasraei et al ( 2017 ) indicated that there was no significant difference in microleakage between the bromelain‐treated and acid‐etched surfaces. Dayem and Tameesh ( 2013 ) revealed that using bromelain enzyme on acid‐etched dentin significantly decreased the microleakage because they used bromelain on acid‐etched dentin and we used bromelain on the dentin.…”

Section: Discussionmentioning

confidence: 99%

Evaluation of the microleakage of class V composite restoration after cavity treatment with Erbium, CO₂ lasers, Papain, and Bromelain enzymes

Sharafeddin,

Tabrizi

2023

Clinical & Exp Dental Res

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ObjectvesDifferent surface preparation and treatment methods may have dissimilar effects on the microleakage of composite resin. This study was conducted to determine the deproteinizing effect of 10% bromelain enzyme, 10% papain enzyme, CO2, and erbium‐YAG laser in regard to decrease in the microleakage of composite restorations.Materials and MethodsThirty teeth were selected and 60 class V cavities were prepared on the lingual and buccal sides. They were divided into six groups (n = 10): Group 1, phosphoric acid gel; Group 2, bromelain enzyme 10%; Group 3, papain enzyme 10%; Group 4, mixed papain and bromelain enzymes 10%; Group 5, CO2 laser; and Group 6, erbium‐YAG laser. They were stored in basic fuchsine and dye penetration was evaluated. Kruskal–Wallis and Mann–Whitney tests were used for statistical analysis, p < 0.05ResultsIn both occlusal and gingival margins, comparison of microleakage between groups 1, 2, 3, 4, and 5 showed no significant differences (p = 1) and group 6 had a significant difference with other groups (p ˂ 0.001).ConclusionsMicroleakage of composite resin in the dentin surface was not affected significantly using either bromelain or papain 10% enzymes or erbium laser. However, CO2 laser had a negative effect on the enamel and dentin margins and increased the microleakage. Erbium laser showed a better effect than enzymes on microleakage.

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“…Different types of direct esthetic restorative materials are available to dentists, including composites and glass ionomer cements 2 . Formulation improvement, leading to the increase in durability of these restorative materials, and made them as the preferable materials for dentists 3 .…”

Section: Introductionmentioning

confidence: 99%

Comparative of flexural strength, hardness, and fluoride release of two bioactive restorative materials with RMGI and composite resin

et al. 2022

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Aim: This study was fulfilled to evaluate the flexural strength, micro-hardness, and release of two fluoride ions of bioactive restorative materials (Cention N and Activa Bioactive), a resin modified glass ionomer (Fuji II LC), and a resin composite (Filtek z250). Methods: Forty samples from four restorative materials (Activa Bioactive, Fuji II LC, Cention N, and Filtek Z250) were provided according to the current standards of ISO 4049/2000 guide lines. Subsequently, the samples were stored for 24 hours and 6 months in artificial saliva, and successively, flexural strength and micro-hardness of the samples were measured. For each studied groups the pH was decreased from 6.8 to 4 in storage solution. The rate of changes in fluoride ion release was measured after three different storage periods of 24 hours, 48 hours, and 6 months in distilled water, according to the previous studies’ method. Two-way ANOVA, One-way ANOVA, Tukey HSD Pair wise comparisons, and independent t-tests were used to analyze data (α= 0.05). Results: The highest flexural strength and surface micro-hardness after 24 hours and also after 6 month were observed for Cention N(p<0.001).Flexural strength of all samples stored for 6 months was significantly lower than the samples stored for 24 hours(p<0.001). The accumulative amount of the released fluoride ion in RMGI, after six-month storage period in distilled water was considerably higher (p<0.001) than 24 hours and 48 hours storage. The amount of fluoride ion release with increasing acidity of the environment (from pH 6.8 to 4) in Fuji II LC glass ionomer was higher than the bioactive materials (p<0.05). Conclusion: The flexural strength of RMGI was increased after storage against the Activa Bioactive,Cention N and Z250 composite. Storage of restorative materials in artificial saliva leads to a significant reduction in micro hardness. The behavior and amount of released fluoride ions in these restorative materials, which are stored in an acidic environment, were dependent on the type of restorative material.

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Effect of Proteolytic Agents on Microleakage of Etch and Rinse Adhesive Systems

Cited by 5 publications

References 29 publications

Effects of different concentrations of bromelain and papain enzymes on shear bond strength of composite resin to deep dentin using an etch-and-rinse adhesive system

Effects of different concentrations of bromelain and papain enzymes on shear bond strength of composite resin to deep dentin using an etch-and-rinse adhesive system

Evaluation of the microleakage of class V composite restoration after cavity treatment with Erbium, CO₂ lasers, Papain, and Bromelain enzymes

Comparative of flexural strength, hardness, and fluoride release of two bioactive restorative materials with RMGI and composite resin

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Effect of Proteolytic Agents on Microleakage of Etch and Rinse Adhesive Systems

Cited by 5 publications

References 29 publications

Effects of different concentrations of bromelain and papain enzymes on shear bond strength of composite resin to deep dentin using an etch-and-rinse adhesive system

Effects of different concentrations of bromelain and papain enzymes on shear bond strength of composite resin to deep dentin using an etch-and-rinse adhesive system

Evaluation of the microleakage of class V composite restoration after cavity treatment with Erbium, CO2 lasers, Papain, and Bromelain enzymes

Comparative of flexural strength, hardness, and fluoride release of two bioactive restorative materials with RMGI and composite resin

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Evaluation of the microleakage of class V composite restoration after cavity treatment with Erbium, CO₂ lasers, Papain, and Bromelain enzymes