Effect of provenance, plant part and processing on extract profiles from cultivated European Rhodiola rosea L. for medicinal use

Peschel, Wieland; Prieto, José M.; Karkour, Caroline; Williamson, E. M.

doi:10.1016/j.phytochem.2012.10.005

Cited by 40 publications

(37 citation statements)

References 6 publications

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“…The aromatic region (δH 6.5-7.2 ppm, H3/5 and H2/6 proton signals for SAL and TYR were 6.982 ppm to 7.009 ppm) and the sugar region (δH 3-4.5 ppm, H −1 proton signal for SAL was 4.218 ppm to 4.248 ppm) were found to contain aromatic compound and glycosidic constituent peaks identical to those found in commercially available SAL and TYR, respectively ( Figure 4). These findings are also consistent with previous studies [4,21] and lend further support to the authenticity of SAL and TYR produced by the Rac56 strain. Under a microscope, the mycelia were observed to be dark with a size range of 1.5-4.5 µm.…”

Section: Validation Of Sal and Tyr Produced From Rac56 By Lc-ms And 1supporting

confidence: 92%

“…These yields were higher than those of any other conditions (Table 4). 3 Medium volume, 4 Rotation speed, 5 Fermentation time, 6 salidroside, 7 p-tyrosol.…”

Section: Fermentation and Optimal Growth Conditioning For Sal And Tyrmentioning

confidence: 99%

“…It was used as food crop and folk medicine by many ancient civilizations because of its pharmacologic effects, such as antioxidant, anti-fatigue, anti-aging, as well as adaptogenic properties [3]. With increasing demand for SAL and TYR as additives in the health food, cosmetic, and pharmaceutical industries, wild Rhodiola plants are being over-exploited [4]. However, artificial cultivation of Rhodiola plants has been limited by the elevation requirement of planting areas, long production cycle, and low content of active components [4].…”

Section: Introductionmentioning

confidence: 99%

“…With increasing demand for SAL and TYR as additives in the health food, cosmetic, and pharmaceutical industries, wild Rhodiola plants are being over-exploited [4]. However, artificial cultivation of Rhodiola plants has been limited by the elevation requirement of planting areas, long production cycle, and low content of active components [4]. In recent years, tissue and cell culture as well as chemical synthesis methods have been used for SAL and TYR production; however, these methods are costly, low yielding, and conducive to pollution [5].…”

Section: Introductionmentioning

confidence: 99%

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Potential of the Endophytic Fungus Phialocephala fortinii Rac56 Found in Rhodiola Plants to Produce Salidroside and p-Tyrosol

et al. 2016

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2-(4-Hydroxyphenyl)ehyl-β-D-glucopyranoside (salidroside) and 4-(2-hydroxyethyl)phenol (p-tyrosol) are famous food and medicine additives originally derived from alpine Rhodiola plants. Salidroside or p-tyrosol production by the endophytic fungus Rac56 (Phialocephala fortinii) was confirmed by UPLC/Q-TOF-MS and 1 H-NMR. The fermentation conditions were optimized by orthogonal design using data processing system software. The broth fermentation results showed that salidroside and p-tyrosol extraction yields from Rac56 were stable and reached 1.729˘0.06 mg and 1.990˘0.05 mg per mL of culture medium, respectively. The optimal conditions for salidroside and p-tyrosol production in fermentation culture of Rac56 were determined to be 25˝C, pH values of 7 and 5, Czapek-Dox culture medium volumes of 150 mL and 50 mL in 250 mL flasks, rotation speeds of 100ˆg and 200ˆg, and fermentation durations of 7 and 15 days, respectively. Under these optimal conditions, stable yields of 2.339˘0.1093 mg and 2.002˘0.0009 mg per mL of culture medium of salidroside and p-tyrosol, respectively, were obtained, indicating that the P. fortinii Rac56 strain is a promising source of these compounds.

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Section: Validation Of Sal and Tyr Produced From Rac56 By Lc-ms And 1supporting

confidence: 92%

“…These yields were higher than those of any other conditions (Table 4). 3 Medium volume, 4 Rotation speed, 5 Fermentation time, 6 salidroside, 7 p-tyrosol.…”

Section: Fermentation and Optimal Growth Conditioning For Sal And Tyrmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Potential of the Endophytic Fungus Phialocephala fortinii Rac56 Found in Rhodiola Plants to Produce Salidroside and p-Tyrosol

et al. 2016

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“…Since Nuodikang is of Tibetan origin, it could contain other species from the Rhodiola genus, which also have tyrosol and salidroside as constituents (Nakamura et al 2008;Lee et al 2013). It is not uncommon in Asian markets that R. rosea products contain mixtures of different species (Brown et al 2002;Peschel et al 2013). However, the CYP inhibition potential of Nuodikang falls within the other R. rosea products, indicating that, despite not being an R. rosea product, it contains similar amounts of other, not measured constituents, responsible for the observed inhibition.…”

Section: Discussionmentioning

confidence: 99%

In vitro inhibition of cytochrome P-450 activities and quantification of constituents in a selection of commercial Rhodiola rosea products

Thu

Nilsen

Hellum

2016

Pharmaceutical Biology

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Context: Rhodiola rosea L. (Crassulaceae) products are popular natural remedies with a worldwide distribution. Recent studies have revealed potent CYP inhibition by R. rosea extracts both in vitro and in vivo, but information on in vitro CYP inhibition by commercial products are lacking. Variations in commercial R. rosea product quality have also been published. Objective: This study evaluates the variation of in vitro CYP inhibition potential and product quality of six commercially available R. rosea products. Materials and methods: Human CYPs isolated from baculovirus-infected cell system were incubated with testosterone (CYP3A4), dextromethorphan (CYP2D6) or phenacetin (CYP1A2). Positive CYP inhibitors ketoconazole (CYP3A4), quinidine (CYP2D6) and b-naphtoflavone (CYP1A2) were used as controls. Quantification of rosavin, rosarin, rosin, tyrosol and salidroside were used to evaluate R. rosea content. Results: IC 50 values ranged from 7.2-106.6 lg/mL for CYP3A4, 13.0-186.1 lg/mL for 2D6 and 10.7-116.0 lg/mL for 1A2. The tincture formulation of R. rosea was the strongest inhibitor giving the lowest IC 50 values of 7.2 ± 0.7, 13 ± 1.7 and 10.7 ± 5.6 lg/mL, respectively. CYP3A4 was significantly more inhibited by the different products than CYP1A2 (p < .05). One of the six products did not contain any rosavin, rosarin or rosin and is not a R. rosea product. Constituent concentrations were not linked to enzyme inhibition. Discussion and conclusion: The present results show a large variation in inhibitory potential between the products. Several of the products demonstrate similar inhibition levels as the product Arctic Root already proven to inhibit CYP enzyme activity in man.ARTICLE HISTORY

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2015

Phytopharmacy

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Effect of provenance, plant part and processing on extract profiles from cultivated European Rhodiola rosea L. for medicinal use

Cited by 40 publications

References 6 publications

Potential of the Endophytic Fungus Phialocephala fortinii Rac56 Found in Rhodiola Plants to Produce Salidroside and p-Tyrosol

Potential of the Endophytic Fungus Phialocephala fortinii Rac56 Found in Rhodiola Plants to Produce Salidroside and p-Tyrosol

In vitro inhibition of cytochrome P-450 activities and quantification of constituents in a selection of commercial Rhodiola rosea products

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