In viticulture, information about vine vigour is a key input for decision-making in connection with production targets. Pruning weight (PW), a quantitative variable used as indicator of vegetative vigour, is associated with the quantity and quality of the grapes. Interest has been growing in recent years around the use of unmanned aerial vehicles (UAVs) or drones fitted with remote sensing facilities for more efficient crop management and the production of higher quality wine. Current research has shown that grape production, leaf area index, biomass, and other viticulture variables can be estimated by UAV imagery analysis. Although SfM lowers costs, saves time, and reduces the amount and type of resources needed, a review of the literature revealed no studies on its use to determine vineyard pruning weight. The main objective of this study was to predict PW in vineyards from a 3D point cloud generated with RGB images captured by a standard drone and processed by SfM. In this work, vertical and oblique aerial images were taken in two vineyards of Godello and Mencía varieties during the 2019 and 2020 seasons using a conventional Phantom 4 Pro drone. Pruning weight was measured on sampling grids comprising 28 calibration cells for Godello and 59 total cells for Mencía (39 calibration cells and 20 independent validation). The volume of vegetation (V) was estimated from the generated 3D point cloud and PW was estimated by linear regression analysis taking V as predictor variable. When the results were leave-one-out cross-validated (LOOCV), the R2 was found to be 0.71 and the RMSE 224.5 (g) for the PW estimate in Mencía 2020, calculated for the 39 calibration cells on the grounds of oblique images. The regression analysis results for the 20 validation samples taken independently of the rest (R2 = 0.62; RMSE = 249.3 g) confirmed the viability of using the SfM as a fast, non-destructive, low-cost procedure for estimating pruning weight.