Effect of psoralen on the regulation of osteogenic differentiation induced by periodontal stem cell-derived exosomes

Abstract

Periodontitis is a chronic inflammatory disease that is the main cause of tooth loss in adults, and the key to periodontitis treatment is the repair and regenerate of periodontal bone tissue. Psoralen is the main component of the Psoralea corylifolia Linn, which shows antibacterial, anti-inflammatoryand osteogenic activities. It promotes the differentiation of periodontal ligament stem cells toward osteogenesis. Exosomes secreted by stem cells play important roles in information transmission during the osteogenic differentiation process. The aim of this paper was to investigate the role of psoralen in regulating osteogenic miRNA information in periodontal stem cells and in periodontal stem cells exosomes and the specific mechanism of its action. Experimental results show that exosomes of human periodontal ligament stem cell origin treated with psoralen (hPDLSCs + Pso-Exos) were not significantly different from untreated exosomes (hPDLSC-Exos) in terms of size and morphology. Thirty-five differentially expressed miRNAs were found to be upregulated and 58 differentially expressed miRNAs were found to be downregulated in the hPDLSCs + Pso-Exos compared to the hPDLSC-Exos (P < 0.05). hsa-miR-125b-5p was associated with osteogenic differentiation. Among them, hsa-miR-125b-5p was associated with osteogenic differentiation. After hsa-miR-125b-5p was inhibited, the osteogenesis level of hPDLSCs was enhanced. In summary, the osteogenic differentiation of hPDLSCs was promoted by psoralen through the downregulation of hsa-miR-125b-5p gene expression in hPDLSCs, and the expression of the hsa-miR-125b-5p gene was also downregulated in exosomes. This finding provides a new therapeutic idea for using psoralen to promote periodontal tissue regeneration.

PSO/SDF-1 composite hydrogel promotes osteogenic differentiation of PDLSCs and bone regeneration in periodontitis rats

“…2 D and F). Furthermore, the effect of 10 μg/mL PSO on the osteogenic ability of PDLSCs has been demonstrated by our previous research [ 38 ]. Therefore, this study determined that the optimal concentration of SDF-1 was 100 ng/mL, and the optimal concentration of PSO was 10 μg/mL.…”

Preparation of an injectable zinc-containing hydrogel with double dynamic bond and its potential application in the treatment of periodontitis

“…Human periodontal ligament stem cells (hPDLSCs) were obtained according to previous studies. 50 hPDLSCs from the periodontal ligament of healthy third molars without any dental and periodontal tissue disease. The third molars used were all from patients (18–25 years old) without systemic diseases.…”

The Genetic Aspects of Periodontitis Pathogenesis and the Regenerative Properties of Stem Cells

“…The stimulation of periodontal stem cells with psoralen, a compound extracted from Psoralea corylifolia, changed the pattern of miRNAs secreted in exosomes. Vesicles derived from stimulated cells were associated with a reduced expression of miR-125b-5p, which is a molecule that inhibits osteogenesis [161].…”