Effect of Puerarin on Expression of ICAM-1 and TNF-α in Kidneys of Diabetic Rats

Pan, Xiaoxia; Wang, Jianfu; Pu, Yanhua; Yao, Jinming; Wang, Huanjun

doi:10.12659/msm.893714

Cited by 20 publications

(10 citation statements)

References 22 publications

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“…ICAM-1 is a multi-functional transmembrane protein, well known for its importance in stabilizing cell-cell interactions, proliferation, and adhesion (Kotteas et al, 2014). Previous studies have indicated that the inhibitory effects of puerarin on ICAM-1 expression were found to not only exist in human umbilical vein endothelial cells (Hu et al, 2010), but also to limit early-stage renal damage in diabetic rats (Pan et al, 2015). The present study showed that PR decreased the levels of ICAM-1 in DMBA-induced breast tumors in rats, an observation supported by the studies described previously.…”

Section: Journal Of Agricultural Sciencesupporting

confidence: 92%

Suppressive Effects of Puerariae Radix on the Breast Tumor Incidence in Rats Treated with DMBA

Chen¹,

Wu²,

Tseng³

et al. 2017

JAS

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Puerariae Radix (PR) is the root of Pueraria lobata, an herb widely used in traditional Chinese medicine and as a food seasoning. Owing to its potential anti-oxidative ability, the effects of PR on the inhibition of breast tumors were investigated in this study using the 7,12-dimethylbenz[a]anthracene (DMBA)-treated rat model. Thirty-two female Sprague-Dawley rats were randomly assigned to one of four different treatment groups, with or without PR and/or DMBA administration. At the age of 177 days, all rats were sacrificed, the number and size of tumors were recorded, and blood and tissue samples were collected for assessment of hematological parameters, redox status, and tumorigenesis-related factors. We found that PR supplementation decreased tumor incidence and WBC count but increased the IgM levels, and significantly altered the redox status, elevated serum TGF-β levels, and reduced serum VEGF-C and ICAM-1 levels. The mechanism by which PR decreased the incidence of DMBA-induced rat breast tumors might include ameliorating immunity, enhancing the antioxidant status, increasing the hepatic excretion of carcinogenic metabolites of DMBA, and influencing the expression of tumorigenesis-related factors.

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Section: Journal Of Agricultural Sciencesupporting

confidence: 92%

Suppressive Effects of Puerariae Radix on the Breast Tumor Incidence in Rats Treated with DMBA

Chen¹,

Wu²,

Tseng³

et al. 2017

JAS

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“…SP can also reduce ICAM-1 and TNF-α levels at the same time to mitigate renal damage in diabetic rats. 17 Additionally, SP can effectively inhibit INF-γ and TGF-β1 expression in renal tissues of diabetic rats. 18 Recent research reveals that miRNAs play a vital role in the diagnosis and treatment of diabetes.…”

Section: Discussionmentioning

confidence: 99%

<p>Standard Puerarin Prevents Diabetic Renal Damage by Inhibiting miRNA-140-5p Expression</p>

Yan

Xiong

et al. 2020

DMSO

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Aim This study was designed to use in vivo and in vitro approaches to evaluate puerarin in diabetes-induced renal injury. Materials and Methods SD rats were divided into NC (normal control), Model (diabetic induced renal injury model), SP-L (model rats treated with low-dose standard puerarin), SP-M (model rats treated with middle-dose standard puerarin), and SP-H (model rats treated with high-dose standard puerarin) groups. We evaluated fasting blood-glucose (FBG), urinary albumin/creatinine ratio (UACR), body weight, and kidney index (KI) in the different groups. TNF-α, IL-1β, and IL-6 concentrations were measured using Elisa assays. HE staining and TUNEL assays were used to evaluate pathology and apoptosis in kidney tissues, respectively. Relative gene and protein expression was measured using RT-qPCR and Western blot assays. Apoptosis was measured using flow cytometry. The correlation between miRNA-145-5p and TLR4 was assessed using dual-luciferase reporter gene assays. Results The pathology and apoptosis cell number were deteriorate in Model group; TNF-α, IL-1β and IL-6 concentrations, FGB, UACR and KI were increased and body weight was depressed; meanwhile, relative gene and proteins expressions (miRNA-145-5p, TLR4, MyD88 and NF-κB p65) were significantly different in Model group in vivo and vitro study compared with NC group. SP treatment significantly improved the pathology and apoptosis levels in the tissues, as well as TNF-α, IL-1β and IL-6 concentrations, FGB, UACR, body weight, and KI. In vitro cell studies revealed that SP could prevent renal injury induced by diabetes through the miRNA-145-5p/TLR4 axis. Conclusion SP prevents diabetes-induced renal damage via miRNA-145-5p overexpression and reduces TLR4/MyD88/NF-κB (p65) pathway activation in vitro and in vivo.

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“…Multiple extracts of XLP can improve diabetes complications [28,29], and icariside II can reduce cognitive deficits in rats with cognitive impairment induced by neurotoxins [30]. Puerarin has hypoglycemic effect and can improve insulin resistance [31] and diabetes complications [32] in diabetic mice, and it can reduce cognitive impairment in precursor protein/presenilin-1 double transgenic model mice [33]. Salidroside has been found to reduce fasting blood glucose and blood lipid levels in diabetic rats, and to prevent cognitive impairment caused by diabetes by regulating the Rho/Rho-associated kinase (ROCK)/sirtuin 1 (SIRT1)/nuclear factor (NF)-κB pathway [9].…”

Section: Discussionmentioning

confidence: 99%

Network Pharmacology Dissection of Multi-scale Mechanisms of Action of Tu-Xian Mixture for the Treatment of Diabetic Cognitive Impairment

Yan

Sun

et al. 2020

Preprint

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Background Traditional Chinese medicine (TCM) has long been used in the treatment of diabetic cognitive impairment (DCI), and Tu-Xian mixture is one of the prescriptions. This study used a network pharmacology method to predict the active ingredients, potential targets and pathways of Tu-Xian mixture in the treatment of DCI.Methods The potential active ingredients of Tu-xian mixture were obtained by querying databases such as TCMSP, drug targets were screened through Pharmmapper, ETCM, and UniProt databases, and disease target protein groups were collected using TTD, OMIM, and CTD databases. Therapeutic targets were obtained by comparative analysis. Then use WebGestalt to complete GO enrichment analysis and KEGG pathway analysis.Results A total of 51 active ingredients and 619 potential targets were obtained, among which there were 33 key targets for diabetes and 18 key targets for cognitive impairment. After GO analysis and KEGG pathway analysis, steroid hormone biosynthesis and γ-aminobutyric acid signaling pathway may be the important metabolic pathways for Tu-Xian mixture to perform therapeutic effects.Conclusions This work promoted the comprehension of Tu-Xian mixture in the treatment of DCI, and further experimental verification of the prediction results was still needed to support its clinical application.

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Effect of Puerarin on Expression of ICAM-1 and TNF-α in Kidneys of Diabetic Rats

Cited by 20 publications

References 22 publications

Suppressive Effects of Puerariae Radix on the Breast Tumor Incidence in Rats Treated with DMBA

Suppressive Effects of Puerariae Radix on the Breast Tumor Incidence in Rats Treated with DMBA

<p>Standard Puerarin Prevents Diabetic Renal Damage by Inhibiting miRNA-140-5p Expression</p>

Network Pharmacology Dissection of Multi-scale Mechanisms of Action of Tu-Xian Mixture for the Treatment of Diabetic Cognitive Impairment

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