Effect of pulsed electric field on soybean isoflavone glycosides hydrolysis by β-glucosidase: Investigation on enzyme characteristics and assisted reaction

Lü, Chao; Li, Fangyu; Yan, Xuemin; Mao, Shuo; Zhang, Tiehua

doi:10.1016/j.foodchem.2021.132032

Cited by 17 publications

(6 citation statements)

References 38 publications

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“…A stronger electric field resulted in a significant increase in p-NP concentration, but frequency had no effect. These results are consistent with those of Lu et al [25], who found a significant increase in β-GLU activity when the electric strength was increased from 5 to 15 kV/cm. However, in samples treated longer (2 and 5 min), the concentration of p-NP was significantly lower than in controls.…”

Section: β-Glucosidasesupporting

confidence: 93%

Innovative Approaches to Enhance Activity of Endogenous Olive Enzymes—A Model System Experiment: Part II—Non-Thermal Technique

Kraljić,

Balbino,

Filipan

et al. 2023

Processes

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The aim of this study was to determine the optimal parameters of pulsed electric field treatment to increase the activity of β-glucosidase and lipoxygenase, crucial enzymes that improve the nutritional quality and sensory properties of virgin olive oils. The activity of the enzymes was determined spectrophotometrically immediately after treatment of model systems (commercial enzymes and their substrates) with pulsed electric fields and after simulation of the malaxation process for 30 min at 25 °C. Pulsed electric field treatments were performed with electric field strengths of 2.67 and 13.33 kV/cm at frequencies of 25 and 125 Hz for 1, 2, and 5 min. The results showed a positive correlation of β-glucosidase activity with the electric field strength and time, with the first of the two factors having the greatest influence. Lipoxygenase activity was affected only by the duration of treatment: positively immediately after treatment and negatively after simulation of the malaxation process. Based on these results, pretreatment with a moderate pulsed electric field for 2 min before the malaxation process could be proposed to increase the activity of β-glucosidase and lipoxygenase, e.g., for the production of virgin olive oils with higher concentrations of polyphenols and desirable volatile compounds.

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Section: β-Glucosidasesupporting

confidence: 93%

Innovative Approaches to Enhance Activity of Endogenous Olive Enzymes—A Model System Experiment: Part II—Non-Thermal Technique

Kraljić,

Balbino,

Filipan

et al. 2023

Processes

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“…Because converting glucosides into aglycones is metabolically beneficial, β-glucosidase is widely used in industry, including in food supplements and feed . Most studies on β-glucosidase have focused on topics such as hydrolysis enhancement and enzyme immobilization for industrial applications. Some novel β-glucosidases have also been developed for this purpose. , However, these β-glucosidases are limited to a few glucoside ISFs, and data on malonyl and acetyl glucosides are lacking.…”

Section: Resultsmentioning

confidence: 99%

Alkaline–Enzymatic Hydrolysis for Improved Quantitation of Total Isoflavones in Soy Protein-Based Infant Food by UPLC–MS/MS

Gu,

Pang,

et al. 2023

ACS Food Sci. Technol.

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Early life exposure to isoflavones may have detrimental effects on the reproductive system. However, determining the total 12 isoflavones found in soybeans is challenging due to the unavailability of reference standards for all isoflavones. In this study, a method was developed using only three aglycone isoflavones to quantitate all isoflavones. Three different β-glucosidases were evaluated to hydrolyze all glucoside isoflavones, but resistance was observed for malonyl and acetyl glucosides. To address this issue, 80% methanol containing 0.3% NaOH was used as the extraction solution and to facilitate the hydrolysis, allowing 0.5 U of βglucosidase from Thermotoga maritima to efficiently hydrolyze all glucoside isoflavones with the corresponding aglycones obtained within 10 min. Combined with ultraperformance liquid chromatography with tandem mass spectrometry (UPLC-MS/MS), efficient quantitation of all isoflavones was achieved with low limits of quantitation (0.010−0.23 μg/g) and good recoveries (97.0−98.6%) for soy protein-based infant food. This developed method is expected to normalize total isoflavones to aglycone equivalents to avoid overestimating isoflavone contents caused by the presence of glucoside forms.

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“…6 c) during induction stage. β-glucosidase can promote the decomposition of isoflavone glycosides [ 83 ], and the decrease of isoflavone metabolic protein accumulation is related to the promotion of AR initiation [ 45 ]. Therefore, it is speculated that MsG0380016110.01 could induce AR production by regulating isoflavone metabolism.…”

Section: Discussionmentioning

confidence: 99%

Uncovering early transcriptional regulation during adventitious root formation in Medicago sativa

Wang³

et al. 2023

BMC Plant Biol

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Background Alfalfa (Medicago sativa L.) as an important legume plant can quickly produce adventitious roots (ARs) to form new plants by cutting. But the regulatory mechanism of AR formation in alfalfa remains unclear. Results To better understand the rooting process of alfalfa cuttings, plant materials from four stages, including initial separation stage (C stage), induction stage (Y stage), AR primordium formation stage (P stage) and AR maturation stage (S stage) were collected and used for RNA-Seq. Meanwhile, three candidate genes (SAUR, VAN3 and EGLC) were selected to explore their roles in AR formation. The numbers of differentially expressed genes (DEGs) of Y-vs-C (9,724) and P-vs-Y groups (6,836) were larger than that of S-vs-P group (150), indicating highly active in the early AR formation during the complicated development process. Pathways related to cell wall and sugar metabolism, root development, cell cycle, stem cell, and protease were identified, indicating that these genes were involved in AR production. A large number of hormone-related genes associated with the formation of alfalfa ARs have also been identified, in which auxin, ABA and brassinosteroids are thought to play key regulatory roles. Comparing with TF database, it was found that AP2/ERF-ERF, bHLH, WRKY, NAC, MYB, C2H2, bZIP, GRAS played a major regulatory role in the production of ARs of alfalfa. Furthermore, three identified genes showed significant promotion effect on AR formation. Conclusions Stimulation of stem basal cells in alfalfa by cutting induced AR production through the regulation of various hormones, transcription factors and kinases. This study provides new insights of AR formation in alfalfa and enriches gene resources in crop planting and cultivation.

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Effect of pulsed electric field on soybean isoflavone glycosides hydrolysis by β-glucosidase: Investigation on enzyme characteristics and assisted reaction

Cited by 17 publications

References 38 publications

Innovative Approaches to Enhance Activity of Endogenous Olive Enzymes—A Model System Experiment: Part II—Non-Thermal Technique

Innovative Approaches to Enhance Activity of Endogenous Olive Enzymes—A Model System Experiment: Part II—Non-Thermal Technique

Alkaline–Enzymatic Hydrolysis for Improved Quantitation of Total Isoflavones in Soy Protein-Based Infant Food by UPLC–MS/MS

Uncovering early transcriptional regulation during adventitious root formation in Medicago sativa

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