Effect of pulsed ultrasound in combination with gentamicin on bacterial viability in biofilms on bone cements in vivo

Ensing, G.T.; Roeder, Beverly L.; Nelson, Jared L.; Horn, J.R. van; Mei, Henny C. van der; Busscher, Henk J.; Pitt, William G.

doi:10.1111/j.1365-2672.2005.02643.x

Cited by 77 publications

(62 citation statements)

References 32 publications

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“…The viability of Escherichia coli biofilms has also been significantly reduced in vivo using pulsed low frequency ultrasound (25% duty, power intensity 500 mW/cm 2 for a treatment period of 24 or 48 h) in combination with gentamicin but these effects were not reproducible against Pseudomonas aeruginosa biofilms [89][90][91]. In 2005, Ensing et al [92] also demonstrated greater than 50% reductions in the viability of Escherichia coli biofilms by combining pulsed ultrasound treatment (24-48 kHz, power intensity 500 mW/cm 2 ) with gentamicin.…”

Section: Biofilm Prevention and Control Using Acoustic Energymentioning

confidence: 99%

Electrical methods of controlling bacterial adhesion and biofilm on device surfaces

Freebairn

Linton

Harkin‐Jones

et al. 2013

Expert Review of Medical Devices

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SummaryThis review will summarize the significant body of research within the field of electrical methods of controlling the growth of microorganisms. We examine the progress from early work using current to kill bacteria in static fluids to more realistic treatment scenarios such as flow-through systems designed to imitate the human urinary tract. Additionally, the electrical enhancement of biocide and antibiotic efficacy will be examined alongside recent innovations including the biological applications of acoustic energy systems to prevent bacterial surface adherence. Particular attention will be paid to the electrical engineering aspects of previous work, such as electrode composition, quantitative electrical parameters, and the conductive medium used. Scrutiny of published systems from an electrical engineering perspective will help to facilitate improved understanding of the methods, devices and mechanisms that have been effective in controlling bacteria, as well as providing insights and strategies to improve the performance of such systems and develop the next generation of antimicrobial bioelectric materials.

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Section: Biofilm Prevention and Control Using Acoustic Energymentioning

confidence: 99%

Electrical methods of controlling bacterial adhesion and biofilm on device surfaces

Freebairn

Linton

Harkin‐Jones

et al. 2013

Expert Review of Medical Devices

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“…However, it is sometimes deficient in antimicrobial efficacy (21). Many authors have attributed this defect to the incomplete release of the antibiotic from the cement (4,5,6,8,20,23,24). The matrix of polymethylmethacrylate is, to a large extent, impermeable to antibiotics.…”

mentioning

confidence: 99%

Effect of Delayed Pulsed-Wave Ultrasound on Local Pharmacokinetics and Pharmacodynamics of Vancomycin-Loaded Acrylic Bone Cement In Vivo

Cai

Yan

et al. 2007

Antimicrob Agents Chemother

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This study sought to investigate the effect of delayed pulsed-wave ultrasound with low frequency on drug release from and the antimicrobial efficacy of vancomycin-loaded acrylic bone cement in vivo and the possible mechanism of this effect. After the implantation of cement and the inoculation of Staphylococcus aureus into the bilateral hips of rabbits, ultrasound (average intensity, 300 mW/cm 2 ; frequency, 46.5 kHz; on/off ratio, 20 min/10 min) was applied to animals in the normal ultrasound group (UG 0-12 ) from 0 through 12 h after surgery and to those in the delayed-ultrasound group (UG 12-24 ) from 12 through 24 h after surgery. The control group (CG) was not exposed to ultrasound. Based on vancomycin concentrations in left hip cavities at projected time intervals, the amount of time during which the local drug concentration exceeded the MIC (T >MIC ) in UG 12-24 was significantly prolonged compared with that in either CG or UG 0-12 , and the ratios between the areas under the concentration-time curves over 24 h and the MIC for UG 0-12 and UG 12-24 were both increased compared with that for CG. The greatest reductions in bacterial densities in both right hip aspirates and right femoral tissues at 48 h were achieved with UG 12-24 . Local hemorrhage in rabbits of UG 0-12 during the 12-h insonation was more severe than that in rabbits of UG 12-24 . Of four variables, the T >MIC and the bioacoustic effect were both identified as parameters predictive of the enhancement of the antimicrobial efficacy of cement by ultrasound. Sustained concentrations above the MIC replaced early high maximum concentrations and long-term subtherapeutic release of the drug, provided that ultrasound was not applied until local hemorrhage was relieved. The enhancement of the antimicrobial efficacy of cement by ultrasound may be attributed to the prolonged T >MIC and the bioacoustic effect caused by ultrasound.Of the patients worldwide undergoing total joint replacement per year, approximately 0.3 to 2.2% develop prosthesis-related infections resulting in devastating surgical failure (23,25). Antibiotic-loaded bone cement is the treatment of choice because of its high local dose and low systemic toxicity compared with those of intravenous antibiotics (23,25). However, it is sometimes deficient in antimicrobial efficacy (21). Many authors have attributed this defect to the incomplete release of the antibiotic from the cement (4,5,6,8,20,23,24). The matrix of polymethylmethacrylate is, to a large extent, impermeable to antibiotics. Not only is the bioavailability of the antibiotic decreased, but the prolonged exposure to the antibiotic also allows selective bacterial resistance to occur (20).Recently, low-frequency ultrasound has been found to enhance the release of gentamicin from cement (4, 8). Two possible mechanisms behind this phenomenon include acoustic streaming and an accelerated rate of mass transfer as a result of stable cavitation and the ultrasonic pressure wave. Also, such enhanced release of gentamicin may contribute to a d...

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“…The subject has been recently reviewed and it was found that both effects depend on factors such as frequency, intensity, materials used for ultrasound diffusion, the presence or absence of cavitation, as well as the type of bacteria [105]. The combined use of therapeutic ultrasound with antibiotics usually decreases bacterial viability in vitro and in vivo [106]. On the other hand, sonication at high frequency is unable to kill bacteria, though some damage to the surrounding tissue is produced [107].…”

Section: Mechanical Action On Biostructuresmentioning

confidence: 99%

Interplay Between Mechanochemistry and Sonochemistry

Cintas

Cravotto

Barge

et al. 2014

Topics in Current Chemistry

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Effect of pulsed ultrasound in combination with gentamicin on bacterial viability in biofilms on bone cements in vivo

Cited by 77 publications

References 32 publications

Electrical methods of controlling bacterial adhesion and biofilm on device surfaces

Electrical methods of controlling bacterial adhesion and biofilm on device surfaces

Effect of Delayed Pulsed-Wave Ultrasound on Local Pharmacokinetics and Pharmacodynamics of Vancomycin-Loaded Acrylic Bone Cement In Vivo

Interplay Between Mechanochemistry and Sonochemistry

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