Effect of pup out® on caspase 3 and interleukin-1B expression in pulp teeth: a paste contained jatropha, sidaguri, and melittin

Abstract

Objective: To examine the expression of caspase-3 and IL-1β following application of Pulp Out® on rabbit's pulp teeth as well as histopathology. Material and Methods:The study was conducted on the maxillary incisors rabbits. The teeth were prepared and Pulp Out® was inserted at the base of prepared cavity, then restored with RM-GIC. After 24 hours, the animals were then euthanized and processed for histopathology and immunohistochemistry evaluation. Results:Our results indicated that Pulp Out®, when administered to the pulp teeth, increased the number and diameter of blood vessels. The expression of caspase-3 and IL-1β showed similarities to the expression of commercial devitalizing agent. Conclusion: Our results revealed that Pulp Out® is a valuable devitalization agent that should be further explored for its safety before clinical application.

Dentin microhardness and porosity of wistar rats teeth following herbal-based devitalizing agent application

“…7 Combination of jatropha sap, root of sidaguri and mellitin, namely Pulp-Out has been reported to cause pulpal cell death following its application into tooth cavity that showed similar cell death of commercial devitalizing agents. 8 In previous in vitro study, a decreased of tooth hardness and erosion were reported which was in line with duration and dosage of Pulp-Out applied. 9 It is assumed that the extracts are able to penetrate through the porosity formed and causing cell death.…”

“…The image of cell death is similar to commercial devitalizing agent, arsen trioxide. 8 However the mechanism of pulpal cell death is not really understood. The finding of previous in vitro study on prepared human teeth showed erosion and a decrease of dentin tooth hardness following pulp out application.…”

Acute toxicity potential and impact on periodontal and periapical tissue of Pulp Out: A paste contained jatropha, sidaguri, and melittin

“…Some herbs have also been studied to have the potency to be developed as a pulp devitalizing agent to devitalize dental nerves in root canal treatment. Our previous study observed that these combined herbal extracts, namely Pulp Out, caused pulpal cell death in animal study following its application in exposed pulp which showed similar image of cell death of commercial pulp devitalizing agent, marked by the presence of changes and lysis in the cell nucleus [5] .…”

“…The Pulp Out paste was prepared according to the previous methods described by Tanumihardja et al [5] . The root of sidaguri and sap of jatropha were collected from Bone, South Sulawesi, Indonesia, in May 2021.…”

“…Inducing cell lysis with Pulp Out relies on the dose used. More cells will experience lysis at greater doses [5] . When administered to the pulp teeth, Pulp Out increase the number and diameter of blood vessels and enhance the expression of caspase-3 and IL- 1β.…”