Effect of purification method of β-chitin from squid pen on the properties of β-chitin nanofibers

Suenaga, Shin; Nikaido, Nozomi; Totani, Kiichiro; Kawasaki, Kazunori; Ito, Yoshihito; Yamashita, K.; Osada, Motonobu

doi:10.1016/j.ijbiomac.2016.06.060

Cited by 32 publications

(22 citation statements)

References 32 publications

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“…according to the method reported in our previous study [22]. In brief, squid pens were treated with 0.1 mol L −1 hydrochloric acid solution for 16 h at 15 °C to remove ash and then treated twice with 1 mol L −1 sodium hydroxide solution for 2 h at 90 °C to remove protein.…”

Section: Methodsmentioning

confidence: 99%

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Effect of acidity on the physicochemical properties of α- and β-chitin nanofibers

Suenaga

Totani

Nomura

et al. 2017

International Journal of Biological Macromolecules

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We have investigated whether acidity can be used to control the physicochemical properties of chitin nanofibers (ChNFs). In this study, we define acidity as the molar ratio of dissociated protons from the acid to the amino groups in the raw chitin powder. The effect of acidity on the physicochemical properties of and -ChNFs was compared. The transmittance and viscosity of the -ChNFs drastically and continuously increased with increasing acidity, while those of the -ChNFs were not affected by acidity. These differences are because of the higher ability for cationization based on the more flexible crystal structure of -chitin than -chitin. In addition, the effect of the acid species on the transmittance of -ChNFs was investigated. The transmittance of -ChNFs can be expressed by the acidity regardless of the acid species, such as hydrochloric acid, phosphoric acid, and acetic acid. These results indicate that the acidity defined in this work is an effective parameter to define and control the physicochemical properties of ChNFs.

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Section: Methodsmentioning

confidence: 99%

“…Previously, we reported that when acid remained in -chitin powder after -chitin purification from squid pen, fine homogeneous -ChNFs can be obtained [22]. However, the details of the effect of the amount of the acid on the physicochemical properties of -ChNFs are not clear.…”

Section: Introductionmentioning

confidence: 97%

Effect of acidity on the physicochemical properties of α- and β-chitin nanofibers

Suenaga

Totani

Nomura

et al. 2017

International Journal of Biological Macromolecules

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“…We employ a solution process and solvent casting method to create the ChNF–silk (CS) hybrids as a large‐area film and/or curvilinear structure, such as contact lens ( Figure ). For the solution process, we use a pure β‐chitin and silk fibroin extracted from squid pen and Bombyx mori cocoon (Figure A), respectively, and HFIP (hexafluoro isopropanol) as the solvent; the extraction protocol for both starting materials are well established and widely available in literatures . HFIP is a good solvent for both chitin and silk fibroin, and allows homogeneous blending of the two in the form of clear CS solutions with varying compositions (CS XY , where X : Y = chitin:silk weight ratio), as shown in Figure B (see the Experimental Section for details).…”

Section: Resultsmentioning

confidence: 99%

Biomimetic Chitin–Silk Hybrids: An Optically Transparent Structural Platform for Wearable Devices and Advanced Electronics

Hong

Choi

Kim

et al. 2017

Adv Funct Materials

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The cuticles of insects and marine crustaceans are fascinating models for man-made advanced functional composites. The excellent mechanical properties of these biological structures rest on the exquisite self-assembly of natural ingredients, such as biominerals, polysaccharides, and proteins. Among them, the two commonly found building blocks in the model biocomposites are chitin nanofibers and silk-like proteins with β-sheet structure. Despite being wholly organic, the chitinous protein complex plays a key role for the biocomposites by contributing to the overall mechanical robustness and structural integrity. Moreover, the chitinous protein complex alone without biominerals is optically transparent (e.g., dragonfly wings), thereby making it a brilliant model material system for engineering applications where optical transparency is essentially required. Here, inspired by the chitinous protein complex of arthropods cuticles, an optically transparent biomimetic composite that hybridizes chitin nanofibers and silk fibroin (β-sheet) is introduced, and its potential as a biocompatible structural platform for emerging wearable devices (e.g., smart contact lenses) and advanced displays (e.g., transparent plastic cover window) is demonstrated.

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“…Materials -Chitin with particle sizes below 100 m was obtained from Yaegaki Bio-industry, Inc. (Himeji, Japan) and used without further purification. -Chitin was purified from squid pens (Todarodes pacificus) after removing ash and protein via its treatment with hydrochloric acid and sodium hydroxide according to our method developed in a previous work (Suenaga et al 2016). The purified -chitin was pulverized to particles with sizes of approximately 100 m using a dry pulverizer (Cyclone Mill, Nippon Steel & Sumikin Fine Technology Co., Ltd., Osaka, Japan).…”

Section: Methodsmentioning

confidence: 99%

Parameters of hydrothermal gelation of chitin nanofibers determined using a severity factor

Suenaga

Osada

2018

Cellulose

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The hydrothermal gelation of and -chitin nanofibers (-and -ChNFs) prepared at neutral and acidic pH was conducted by heating them to 120, 160, 180, and 200 °C in a sealed reactor. The optical transmittance and mechanical strength of -ChNFs gelated at the acidic pH were determined for the first time using a severity factor defined as a function of the integrated heating time and temperature. The width of -ChNFs increased after the hydrothermal treatment, indicating that these fibers strongly adhered to each other to form a network structure during gelation. Furthermore, the hydrothermal gelation of and -ChNFs with different degrees of disintegration prepared at the neutral and acidic pH was conducted. It was found that the hydrothermal treatment of -chitin must be performed at the acidic pH to obtain a self-sustaining hydrogel of well-disintegrated NFs. The disintegration of -chitin into NFs occurred more easily at the acidic pH than under the neutral conditions; however, in the latter case, the same disintegration degree of -ChNFs could be achieved by increasing the number of disintegration steps. At the same disintegration degree, the strength of the self-sustaining hydrogel obtained at the neutral conditions was greater than that of the gel prepared at the acidic pH, indicating that the electrostatic repulsion caused by acid addition negatively affected the formation of the hydrogel network structure. To maximize the efficiency of the hydrothermal gelation process, ChNFs should be as thin as possible and electrostatic repulsion forces must be controlled.

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Effect of purification method of β-chitin from squid pen on the properties of β-chitin nanofibers

Cited by 32 publications

References 32 publications

Effect of acidity on the physicochemical properties of α- and β-chitin nanofibers

Effect of acidity on the physicochemical properties of α- and β-chitin nanofibers

Biomimetic Chitin–Silk Hybrids: An Optically Transparent Structural Platform for Wearable Devices and Advanced Electronics

Parameters of hydrothermal gelation of chitin nanofibers determined using a severity factor

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