Effect of purified llama ovulation-inducing factor (OIF) on ovarian function in cattle

Tanco, Valeria M; Steelandt, M. D. Van; Ratto, M.H.; Adams, Gregg P.

doi:10.1016/j.theriogenology.2012.03.036

Cited by 39 publications

(31 citation statements)

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“…X-ray diffraction data were used to solve the full sequence and structure of OIF as β-NGF. Neurite development and up-regulation of trkA in phaeochromocytoma (PC 12 ) cells in vitro confirmed NGF-like properties of OIF. Western blot analysis of llama and bull seminal plasma confirmed immunorecognition of OIF using polyclonal mouse anti-NGF, and administration of β-NGF from mouse submandibular glands induced ovulation in llamas.…”

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confidence: 60%

“…Confirmation by PC 12 Bioassay. Immortalized rat phaeochromocytoma cells (PC 12 ) have trkA receptors on their cell surface that bind specifically with NGF, and neurite growth in PC 12 cells in response to treatment is used as an in vitro bioassay for NGF (15).…”

Section: Resultsmentioning

confidence: 96%

“…Immortalized rat phaeochromocytoma cells (PC 12 ) have trkA receptors on their cell surface that bind specifically with NGF, and neurite growth in PC 12 cells in response to treatment is used as an in vitro bioassay for NGF (15). Treatment of PC 12 cells with purified OIF (fraction C 2 of llama seminal plasma) induced neurite outgrowth in a fashion similar to that of recombinant mouse NGF after 6 d of in vitro culture (Fig.…”

Section: Resultsmentioning

confidence: 98%

“…Furthermore, OIF in seminal plasma influenced ovarian function in species considered to be spontaneous ovulators. That is, OIF induced ovulation in a prepubertal mouse model (11) and altered ovarian follicular wave dynamics in cows (12).…”

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confidence: 99%

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The nerve of ovulation-inducing factor in semen

Ratto

Leduc

Valderrama

et al. 2012

Proc. Natl. Acad. Sci. U.S.A.

140

112

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A component in seminal fluid elicits an ovulatory response and has been discovered in every species examined thus far. The existence of an ovulation-inducing factor (OIF) in seminal plasma has broad implications and evokes questions about identity, tissue sources, mechanism of action, role among species, and clinical relevance in infertility. Most of these questions remain unanswered. The goal of this study was to determine the identity of OIF in support of the hypothesis that it is a single distinct and widely conserved entity. Seminal plasma from llamas and bulls was used as representative of induced and spontaneous ovulators, respectively. A fraction isolated from llama seminal plasma by column chromatography was identified as OIF by eliciting luteinizing hormone (LH) release and ovulation in llamas. MALDI-TOF revealed a molecular mass of 13,221 Da, and 12-23 aa sequences of OIF had homology with human, porcine, bovine, and murine sequences of β nerve growth factor (β-NGF). X-ray diffraction data were used to solve the full sequence and structure of OIF as β-NGF. Neurite development and up-regulation of trkA in phaeochromocytoma (PC 12 ) cells in vitro confirmed NGF-like properties of OIF. Western blot analysis of llama and bull seminal plasma confirmed immunorecognition of OIF using polyclonal mouse anti-NGF, and administration of β-NGF from mouse submandibular glands induced ovulation in llamas. We conclude that OIF in seminal plasma is β-NGF and that it is highly conserved. An endocrine route of action of NGF elucidates a previously unknown pathway for the direct influence of the male on the hypothalamo-pituitary-gonadal axis of the inseminated female.neurotrophins | hypothalamus | fertility | neuroendocrine I n a monograph nearly 50 y ago, Thaddeus Mann summarized the natural properties of seminal plasma as a vehicle for sperm transport, a controller of sperm motility and capacitation, and as a stimulant of uterine contractility (1). Notwithstanding Mann's admonishment to resist the temptation "to assign to every newly discovered chemical constituent of semen a major role in the process of fertilization," recent isolation of a protein factor in seminal plasma (2-4) suggests an additional role of the ejaculateas an inducer of ovulation.The role of the fluid portion of the ejaculate, and the male accessory glands responsible for producing it, has been enigmatic. From an evolutionary perspective, it has been suggested that the male accessory glands likely originated as the machinery for producing a copulatory plug, which has the "chastity effect" of preventing the sperm of other males from entering the female tract, as well as minimizing sperm loss after insemination (5). If this is so, then the persistence of an elaborate accessory gland system in many species in which plug formation does not occur may be viewed as nothing more than an evolutionary vestige.The first reports of an ovulation-inducing factor (OIF) in semen resulted from the observation that ovulation occurred after intravaginal or intramuscula...

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confidence: 60%

Section: Resultsmentioning

confidence: 96%

Section: Resultsmentioning

confidence: 98%

mentioning

confidence: 99%

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The nerve of ovulation-inducing factor in semen

Ratto

Leduc

Valderrama

et al. 2012

Proc. Natl. Acad. Sci. U.S.A.

140

112

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“…It is an effective stimulator of luteinizing hormone (LH) secretion and it effects on ovulation, forming and function of the corpus luteum and acts via a systemic rather than a local pathwayat physiologically relevant doses (Ratto et al, 2006;Fernandez-Baca et al, 1970;Chen et al, 1985). Although the discovery of OIF in seminal plasma of species and categorized as induced ovulators (camel , cat and rabbit), the recent studies support the hypothesis that the OIF in seminal plasma isolated from other species which considered as spontaneous ovulators (cattle horse) (Bogle et al, 2011;Tanco et al, 2012.…”

Section: Advances In Animal and Veterinary Sciencesmentioning

confidence: 99%

Overview of New Concepts in Induce Ovulation Triggers in Dromedary Camels

Al-Fatlawy¹,

Baiee²

2018

Adv. Anim. Vet. Sci.

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IntroductIonH ypothalamuses canpulsatile release the GnRH into the hypophyseal portal system with subsequent release of LH from the anterior pituitary into systemic circulation for inducing ovulation in mammals. The broad classification of species as either spontaneous or induced ovulators is based on the type of stimulus responsible for eliciting GnRH release from the hypothalamus (Adam et al., 2005). In spontaneously ovulating species (such as human, sheep, cattle, horse and pigs), the releasing of GnRH from the hypothalamus is triggered in the absence of progesterone and systemic estradiol concentrations exceed a threshold. In the induced ovulators (such as rabbits, ferrets, cats and camelids) the releasing of GnRH is contingent upon copulatory stimuli; hence, the ovulation is not a regular cyclic event (Bakker and Baum, 2000; Adam et al., 2005). Since a classic 1970 Peruvian study, dogma has maintained that physical stimulation of the genitalia during copulation is the primary trigger for inducing ovulation in alpacas and llamas. It is inaccurate to apply the term "oestrous cycle" in camels due to the pattern of ovulation in these animals,in contrast in spontaneous ovulators such as the cow and the mare (Bravo et al., 1990;Bravo et al., 1991).Camels are seasonally polyestrous animals with estrous cycle differed from that in other farm animals. The estrous cycle in camel is characterized by three phases lasting 24-28 days with absence of the luteal phase. These phases are including follicular growth, existence of mature follicles (estrous period) and follicular atresia. The estrous period is longer up to 8 days (Shalash, 1980;Shalash, 1987;Skidmore et al., 1996;Skidmore et al., 2009).Ovulation in the camel as in cat and rabbit occurs normally after the coitus. In these animals the neuroendocrine reflex involving the initiation of luteinising hormone release is delayed until coitus occurs . Manual stimulation of the cervix for 15 minutes in the camel did not induce ovulation but cause only partial luteinisation of the Graafian follicle, ovulation occurs 32 to 40 hours after copulation under the influence of luteinising hormone (LH) (Musa & Abu Sineina, 1978b;Wilson, 1984;Yagil, 1985). OvulatiOn induced FactOr (OiF): discOvery research ArticleAbstract | The seminal plasma consists of several components that stimulate the ovulation process. The great quantity of an ovulation-inducing factor (OIF) in seminal plasma has wide implication questions about identification, sources, mechanism of action, role among species and clinical applications in the infertility. The purpose of the current review is to focus on the current understanding of physiological and biochemical properties of seminal plasma in camelids. llamas and alpaca seminal plasma was used as agent of induced and spontaneous ovulators. Column chromatography was used to identify the ovulation-inducing factor as part of seminal plasma that stimulating hormone secretion (LH) and ovulation in llamas. OIF is β-NGF that is highly conserved. An endocrine rout...

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Induced Ovulation

Burch¹

2019

Encyclopedia of Evolutionary Psychological Science

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Effect of purified llama ovulation-inducing factor (OIF) on ovarian function in cattle

Cited by 39 publications

References 32 publications

The nerve of ovulation-inducing factor in semen

The nerve of ovulation-inducing factor in semen

Overview of New Concepts in Induce Ovulation Triggers in Dromedary Camels

Induced Ovulation

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