Effect of puromycin on ribosomal particles from streptococcus pyogenes

Scrimshaw, George F.; Bubela, B.; Oberhauser, David F.

doi:10.1016/0024-3205(66)90049-x

Cited by 2 publications

(1 citation statement)

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“…Evidence was found also for a possible factor involved in determining the number of generations which will grow in a specified medium under a given set of conditions. The importance of standardizing experimental conditions during bacterial growth has been discussed elsewhere Scrimshaw, Bubela, and Oberhauser 1966;Bubela, Scrimshaw, and Oberhauser 1967). To obtain meaningful results an apparatus for controlled growth of the microorganisms and a standardized experimental method had to be devised.…”

Section: Introductionmentioning

confidence: 99%

Effects of Temperature on Growth Characteristics of Bacillus Stearothermophlus

Bubela¹

1968

Aust. Jnl. Of Bio. Sci.

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SummaryThe maximum temperature at which B. 8tearothermophilu8 will grow is considerably decreased if a simple medium free of cofactors is used.Such an increase of thermosensitivity could be caused by an imbalance of the cellular system or a shortage of essential components which the organisms cannot produce from the simple medium at all or at the rate required, or by both of these factors.The length of the lag period is determined probably by both the adaptation mechanism to a different temperature and the recovery period from a thermal shock during the harvesting procedure.The number of generations which will grow in a simple, defined medium (MgS04 .7H20 0·08%, CaCh.2H20 0·018%, KN0 3 0·05%, NH4CI 0·28%, glycerol 4%, sodium glycerophosphate O· 6%, and glucose monohydrate 1 % in distilled water) at a given temperature is probably determined by the presence of an inhibitory mater:~l produced and sustained inside the cell as well as other factors. If the rate of formation of such a material is temperature-dependent, growth would be limited to fewer generations at higher temperatures. Experimental evidence supports such a suggestion.

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Section: Introductionmentioning

confidence: 99%