Effect of Pyocyanin on gene expression of norA gene in Staphylococcus aureus and SMR gene in Serratia marcescense

Abstract

Background: The antibiotics active against Multidrug resistant bacteria have several limitations for their use, which include less clinical experience, During the continuing COVID-19 epidemic, limiting use has been difficult because most hospitalized COVID-19 patients receive antibiotics as a defense against subsequent bacterial infection. , Efflux pumps remove drugs and are linked to biofilm formation, making infections more difficult to treat due to a lack of therapeutic choices and necessitating new antimicrobial tactics. Pyocyanin is a secondary blue redox-active metabolite and a large family member of phenazines, tricyclic compounds. Phenazine has been utilized as a biological pesticide against several harmful microorganisms because of its antibacterial properties. Methods: Total RNA from ( Staphylococcus aureus obtained from respiratory tract diagnosed as MDR and Serratia marcescens obtained from co-infection Covid 19 )were isolated . The gene expression of the norA gene efflux pump of S. aureus, which was normalized to the rpoB HKG, and the smr gene efflux pump of Serratia marcescens, which was normalized to the Adnalyate Kinaze enzyme, was studied using quantitative real-time RT PCR on the extracted RNA.