Effect of pyruvate on glucose metabolism in Clostridium acetobutylicum

Junelles, Anne-Marie; Janati‐Idrissi, Rachid; Petitdemange, H.; Gay, Robert

doi:10.1016/0300-9084(87)90145-3

Cited by 12 publications

(4 citation statements)

References 15 publications

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“…The major products of pyruvate fermentation by C. acetobutylicum are acetate, butyrate and butanol, and neither acetate nor butyrate is reutilized. The effects of pyruvate on glucose fermentation by C. acetobutylicum have also been investigated before, and it has been shown that both substrates can be fermented simultaneously [ 40 ]. Furthermore, cellobiose and glucose were only detected at the early stage of batches, which could have been present in the pre-cultures, inoculated into the bioreactors, and were taken up in 24 hours.…”

Section: Resultsmentioning

confidence: 99%

Characterizing metabolic interactions in a clostridial co-culture for consolidated bioprocessing

Salimi

Mahadevan

2013

BMC Biotechnol

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BackgroundClostridial co-culture containing cellulolytic and solventogenic species is a potential consolidated bioprocessing (CBP) approach for producing biochemicals and biofuels from cellulosic biomass. It has been demonstrated that the rate of cellulose utilization in the co-culture of Clostridium acetobutylicum and Clostridium cellulolyticum is improved compared to the mono-culture of C. cellulolyticum (BL 5:119-124, 1983). However, the metabolic interactions in this co-culture are not well understood. To investigate the metabolic interactions in this co-culture we dynamically characterized the physiology and microbial composition using qPCR.ResultsThe qPCR data suggested a higher growth rate of C. cellulolyticum in the co-culture compared to its mono-culture. Our results also showed that in contrast to the mono-culture of C. cellulolyticum, which did not show any cellulolytic activity under conditions similar to those of co-culture, the co-culture did show cellulolytic activity even superior to the C. cellulolyticum mono-culture at its optimal pH of 7.2. Moreover, experiments indicated that the co-culture cellulolytic activity depends on the concentration of C. acetobutylicum in the co-culture, as no cellulolytic activity was observed at low concentration of C. acetobutylicum, and thus confirming the essential role of C. acetobutylicum in improving C. cellulolyticum growth in the co-culture. Furthermore, butanol concentration of 350 mg/L was detected in the co-culture batch experiments.ConclusionThese results suggest the presence of synergism between these two species, while C. acetobutylicum metabolic activity significantly improves the cellulolytic activity in the co-culture, and allows C. cellulolyticum to survive under harsh co-culture conditions, which do not allow C. cellulolyticum to grow and metabolize cellulose independently. It is likely that C. acetobutylicum improves the cellulolytic activity of C. cellulolyticum in the co-culture through exchange of metabolites such as pyruvate, enabling it to grow and metabolize cellulose under harsh co-culture conditions.

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Section: Resultsmentioning

confidence: 99%

Characterizing metabolic interactions in a clostridial co-culture for consolidated bioprocessing

Salimi

Mahadevan

2013

BMC Biotechnol

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“…In this section, we had attempted to study the effect of varied amount of glucose on clostridial fermentation and production of butanol from C. acetobutylicum MTCC 11274. Different concentrations of glucose (20,30,40, 50, 60, 70, and 80 g/l) were supplemented to P2 medium and production of biobutanol was obtained by the OVAT approach. Fermentation was performed for 96 hours at pH 7 and a temperature of 30ᵒC.…”

Section: Effect Of Varying Carbon Sources On Biobutanol Production From C Acetobutylicum Mtcc 11274mentioning

confidence: 99%

“…Cells use it as a source of energy and a metabolic intermediate. Glucose supplemented to complex bacterial growth media is expected to affect the production of a certain protein under the temperature control, which should further affect the ABE solvents yield [20,21].…”

Section: Effect Of Varying Carbon Sources On Biobutanol Production From C Acetobutylicum Mtcc 11274mentioning

confidence: 99%

Enhanced biobutanol production by optimization of the medium from Clostridium acetobutylicum MTCC 11274 using macroalgae Gracilaria edulis

2019

Jabb

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Clostridial strain Clostridium acetobutylicum MTCC 11274 was employed for producing biobutanol in batch culture fermentation. The effects of various carbon sources, i.e., xylose, starch, dextrin, glucose, and mannose as well as nitrogen sources, i.e., yeast extract, peptone, beef extract, and soya protein were studied conventionally (one-factor-at-a-time). It was found that the maximum amount of biobutanol, i.e., 6.27 and 7.40 g/l was obtained from 60 g/l glucose and 5 g/l yeast extract, respectively. In addition to this, the interactions between pH, temperature, and glucose concentration were also taken into consideration for the optimization of biobutanol production with the help of Central Composite Design (CCD) of Response Surface Methodology. CCD design was used for the optimization of the above-mentioned parameters and low and high values of variables were chosen by performing the steepest ascent experiment. The analysis of variance (ANOVA) model was used for estimating the significance of the model coefficients. ANOVA revealed that the model was significant (p < 0.05) and the effects of the glucose concentration, pH, and temperature on biobutanol production were significant. It was found that 8.56 g/l biobutanol was produced under optimum fermentation conditions with 40 g/l Gracilaria edulis supplemented with 20 g/l glucose as a carbon source.

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“…The same behavior has been seen in uncharacterized consortia from anaerobic digesters where increased N 2 sparging enhanced hydrogen production (Mizuno et al, ; Zhang et al, ) and could lead to an enrichment of Clostridium species (Kim et al, ). In addition, C. butyricum growth was inhibited by accumulated organic acids and/or reduced products such as 1,3‐propanediol (Colin et al, ; Zeng et al, ), while pyruvate addition could disrupt the acetogenic to solventogenic transition of C. acetobutylicum (Junelles et al, ).…”

Section: Introductionmentioning

confidence: 99%

Overflow metabolism and growth cessation in Clostridium thermocellum DSM1313 during high cellulose loading fermentations

Thompson

Trinh

2017

Biotech & Bioengineering

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As a model thermophilic bacterium for the production of second-generation biofuels, the metabolism of Clostridium thermocellum has been widely studied. However, most studies have characterized C. thermocellum metabolism for growth at relatively low substrate concentrations. This outlook is not industrially relevant, however, as commercial viability requires substrate loadings of at least 100 g/L cellulosic materials. Recently, a wild-type C. thermocellum DSM1313 was cultured on high cellulose loading batch fermentations and reported to produce a wide range of fermentative products not seen at lower substrate concentrations, opening the door for a more in-depth analysis of how this organism will behave in industrially relevant conditions. In this work, we elucidated the interconnectedness of overflow metabolism and growth cessation in C. thermocellum during high cellulose loading batch fermentations (100 g/L). Metabolic flux and thermodynamic analyses suggested that hydrogen and formate accumulation perturbed the complex redox metabolism and limited conversion of pyruvate to acetyl-CoA conversion, likely leading to overflow metabolism and growth cessation in C. thermocellum. Pyruvate formate lyase (PFL) acts as an important redox valve and its flux is inhibited by formate accumulation. Finally, we demonstrated that manipulation of fermentation conditions to alleviate hydrogen accumulation could dramatically alter the fate of pyruvate, providing valuable insight into process design for enhanced C. thermocellum production of chemicals and biofuels. Biotechnol. Bioeng. 2017;114: 2592-2604. © 2017 Wiley Periodicals, Inc.

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Effect of pyruvate on glucose metabolism in Clostridium acetobutylicum

Cited by 12 publications

References 15 publications

Characterizing metabolic interactions in a clostridial co-culture for consolidated bioprocessing

Characterizing metabolic interactions in a clostridial co-culture for consolidated bioprocessing

Enhanced biobutanol production by optimization of the medium from Clostridium acetobutylicum MTCC 11274 using macroalgae Gracilaria edulis

Overflow metabolism and growth cessation in Clostridium thermocellum DSM1313 during high cellulose loading fermentations

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