Effect of Pythium oligandrum and poly-beta-hydroxy butyric acid application on root growth, forage yield and root diseases of red clover under field conditions

Pisarčik, Martin; Hakl, J.; Hrevušová, Zuzana

doi:10.1016/j.cropro.2019.104968

Cited by 15 publications

(18 citation statements)

References 42 publications

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“…Opportunities exist to achieve accurate and varied lucerne sowing rates according to small‐scale environmental differences, even within a particular field, through the use of precision agriculture technologies, as shown previously in several other crops such as maize (Hörbe et al., 2013). Other techniques can be useful for improved emergence under more precise plant spatial arrangements, such as seed coating (Bicakci et al., 2018) or application of preparations for stimulating growth of forage legumes (Pisarčik et al., 2020). However, before these potential innovations can be adopted, further research is needed that combines the effects of sowing rates and plant distribution with root morphology measurement, together with forage yield and yield components, and particularly with a focus on stressed environments.…”

Section: Discussionmentioning

confidence: 99%

Potential of lucerne sowing rate to influence root development and its implications for field stand productivity

Hakl

Pisarčik

Fuksa

et al. 2021

Grass and Forage Science

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The relationship between root morphology and productivity in lucerne (Medicago sativa L.) has been well documented, but the effect of sowing rate on root development is unclear. Results from three field experiments were analysed to address these objectives: (a) to investigate lucerne root development as a function of distance among plants, (b) to evaluate the effect of sowing rates on lucerne root traits and (c) to investigate the relationship between root traits and forage yield and yield components. A 4‐year experiment that compared spaced plants (50 × 50 cm, four plants m2) versus row seeding (12.5 cm, 700 germinated seeds m−2) revealed fast annual tap‐root diameter (TD) development in spaced plants, with about 12 mm, and three times greater TD and five times the lateral root number compared with row‐spaced plants. Three‐year field plot experiments at one site compared sowing rates of 400 versus 700 germinated seeds m−2 in less favourable soil for lucerne, and 700 versus 900 seeds m−2 in a better soil. Across experiments, higher plant density and root potential index were positively related to forage yield only when in association with better soil conditions and optimal water supply. Lower plant density offered advantages for faster root development. An effect of reduction in the sowing rate was increased stand height and increased yield in the third cut in a year with drought stress. Results show the need for investigation of effects of more precise spatial arrangement of lucerne plants to ensure optimization of root development and to enable yield benefits and drought resistance.

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Section: Discussionmentioning

confidence: 99%

Potential of lucerne sowing rate to influence root development and its implications for field stand productivity

Hakl

Pisarčik

Fuksa

et al. 2021

Grass and Forage Science

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“…It also produces antimicrobial compounds, competes for nutrients and space with pathogens, and promotes plant growth via producing the auxin precursor tryptamine [6,9,10]. Thus, P. oligandrum has been successfully used in agriculture for plant disease control [6,11,12]. Similar to pathogenic oomycetes, P. oligandrum rapidly infects plant root tissues.…”

Section: Introductionmentioning

confidence: 99%

Type 2 Nep1-Like Proteins from the Biocontrol Oomycete Pythium oligandrum Suppress Phytophthora capsici Infection in Solanaceous Plants

Yang

Dong

et al. 2021

JoF

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As a non-pathogenic oomycete, the biocontrol agent Pythium oligandrum is able to control plant diseases through direct mycoparasite activity and boosting plant immune responses. Several P. oligandrum elicitors have been found to activate plant immunity as microbe-associated molecular patterns (MAMPs). Necrosis- and ethylene-inducing peptide 1 (Nep1)-like proteins (NLPs) are a group of MAMPs widely distributed in eukaryotic and prokaryotic plant pathogens. However, little is known about their distribution and functions in P. oligandrum and its sister species Pythium periplocum. Here, we identified a total of 25 NLPs from P. oligandrum (PyolNLPs) and P. periplocum (PypeNLPs). Meanwhile, we found that PyolNLPs/PypeNLPs genes cluster in two chromosomal segments, and our analysis suggests that they expand by duplication and share a common origin totally different from that of pathogenic oomycetes. Nine PyolNLPs/PypeNLPs induced necrosis in Nicotiana benthamiana by agroinfiltration. Eight partially purified PyolNLPs/PypeNLPs were tested for their potential biocontrol activity. PyolNLP5 and PyolNLP7 showed necrosis-inducing activity in N. benthamiana via direct protein infiltration. At sufficient concentrations, they both significantly reduced disease severity and suppressed the in planta growth of Phytophthora capsici in solanaceous plants including N. benthamiana (tobacco), Solanum lycopersicum (tomato) and Capsicum annuum (pepper). Our assays suggest that the Phytophthora suppression effect of PyolNLP5 and PyolNLP7 is irrelevant to reactive oxygen species (ROS) accumulation. Instead, they induce the expression of antimicrobial plant defensin genes, and the induction depends on their conserved nlp24-like peptide pattern. This work demonstrates the biocontrol role of two P. oligandrum NLPs for solanaceous plants, which uncovers a novel approach of utilizing NLPs to develop bioactive formulae for oomycete pathogen control with no ROS-caused injury to plants.

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“…Red clover (Trifolium pratense L.; RC) is one of the most important leguminous plants of temperate zones in Europe and Asia, and it is also a short-term perennial herb (1,2). RC contains high levels of isoflavones (genistein, daidzein, biochanin A, and formononetin) (3).…”

Section: Introductionmentioning

confidence: 99%

Red Clover Isoflavones Influence Estradiol Concentration, Exercise Performance, and Gut Microbiota in Female Mice

Chen

Wang

Zhu³

et al. 2021

Front. Nutr.

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In red clover (Trifolium pratense L.; RC) the main compound is isoflavones, which are selective estrogen receptor modulators for maintaining female health. Isoflavones exert antifatigue effects during exercise in high-temperature environments. This study aimed to investigate the effect of RC supplementation on gut microbiota composition to determine whether it improves intestinal barrier function and exercise performance. Female ICR mice were divided into four groups (n = 8 per group) and orally administered RC once daily for 6 weeks at 0 (vehicle), 308 (RC-1X), 615 (RC-2X), and 1,538 (RC-5X) mg/kg. RC supplementation decreased the fat mass and increased exhaustive swimming time, grip strength, and muscle glycogen in female mice. In the RC supplementation group, serum levels of lactate, ammonia, and creatine kinase decreased after swimming. The estradiol and progesterone levels were higher in the RC group than in the vehicle group. Regarding gut microbiota composition, the RC-2X group may increase intestinal health related to the microorganisms Pseudobutyrivibrio and Parabacteroide. Thus, the use of RC supplements as nutraceuticals could have positive effects on athletes' gut and overall health.

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Effect of Pythium oligandrum and poly-beta-hydroxy butyric acid application on root growth, forage yield and root diseases of red clover under field conditions

Cited by 15 publications

References 42 publications

Potential of lucerne sowing rate to influence root development and its implications for field stand productivity

Potential of lucerne sowing rate to influence root development and its implications for field stand productivity

Type 2 Nep1-Like Proteins from the Biocontrol Oomycete Pythium oligandrum Suppress Phytophthora capsici Infection in Solanaceous Plants

Red Clover Isoflavones Influence Estradiol Concentration, Exercise Performance, and Gut Microbiota in Female Mice

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