Effect of Quercetin and Its Conjugated Metabolite on the Hydrogen Peroxide-induced Intracellular Production of Reactive Oxygen Species in Mouse Fibroblasts

Shirai, Mutsuko; Yamanishi, Rintaro; Moon, Jae‐Hak; Murota, Kazuo; Terao, Junji

doi:10.1271/bbb.66.1015

Cited by 57 publications

(39 citation statements)

References 32 publications

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“…After the incubation of 10 mM quercetin with 3T3 fibroblasts for 4 h, isorhamnetin (3,4Ј,5,7-tetrahydroxy-3Ј-methoxyflavone) and glucuronide/sulfate conjugates of isorhamnetin had accumulated in the medium, but the level of isorhamnetin including the metabolites was quite low in cellular fractions including cell membranes. 33) By contrast, substantial amounts (ca. 6% of the initial content) of quercetin and its glucuronide/sulfate conjugates were present in cellular fraction.…”

Section: Discussionmentioning

confidence: 99%

Quercetin-Induced PC12 Cell Death Accompanied by Caspase-Mediated DNA Fragmentation

Sasaki

Nakamura

Tsuchiya

et al. 2007

Biological & Pharmaceutical Bulletin

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Flavonoids, a large group of polyphenolic compounds found in fruits and vegetables, have attracted much attention because of their broad pharmacological, particularly antioxidative, activities.1,2) Flavonoids may exert beneficial effects on a number of disease states including cancer, cardiovascular disease, inflammation and neurodegenerative disorders. 1,2)The pathology of neurodegenerative disorders including Alzheimer's disease and aging is associated with oxidative stress caused by reactive oxygen species and reactive nitrogen species.2,3) If oxidative stress is suppressed by compounds in foods, such as flavonoids, this may act as a preventive measure to reduce damage and/or disfunction of cells. Quercetin (3,3Ј,4Ј,5,, which possesses a wide spectrum of pharmacological and biological properties, 2,4) is found in many kinds of food. The daily Western diet contains on average about 25 mg of various flavonoids, of which quercetin is a major ingredient (16 mg/d).5) Quercetin can scavenge free radicals directly and inhibit the oxidation of various molecules resulting in the activation of antioxidant defense pathways in vivo and in vitro. However, the actions of flavonoids are complex and paradoxical in some cases. Several studies have shown that quercetin is a pro-oxidant and cytotoxic in leukemic cells. 7,8) Flavonoids including quercetin caused apoptosis in carcinoma/cancer cells 2,9) and showed cytotoxicity in normal cells including human umbilical vein endothelial cells.10) The neuronal density of the hippocampus in gerbils subjected to infusion of quercetin to the ventricle was significantly lower than that in control.11) Thus, the effects of quercetin on cell death and/or apoptosis in cells including neuronal cells have not been well confirmed.The PC12 cell line, a rat adrenal medullary pheochromocytoma, is a useful model for studying the survival of neuronal cells. Native PC12 cells divide and resemble precursors of adrenal chromaffin cells and sympathetic neurons when grown in the presence of serum, and gradually attain the phenotypic properties of sympathetic neurons on the addition of nerve growth factor (NGF). Previously, we reported that the treatment of native and undifferentiated PC12 cells with an inhibitor of thioredoxin reductase and dopaminergic neurotoxins such as 1-methyl-4-phenylpyridinium ion (MPP ϩ ) caused apoptosis via a caspase-mediated pathway. 12,13) Quercetin has been shown to inhibit cell death and/or apoptosis induced by various stimuli including oxidative stress and MPP ϩ in differentiated PC12 cells. [14][15][16] In undifferentiated PC12 cells, however, antioxidants including quercetin did not have a protective effect against cell death induced by oxidative stress including MPP ϩ . 15,16) The effect of quercetin on cell survival and death in undifferentiated PC12 cells has not been well established. In the present study, we investigated whether quercetin causes cell death in undifferentiated PC12 cells or not. The effect of genistein, a soy isoflavone that has the pro-apoptotic ac...

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Section: Discussionmentioning

confidence: 99%

Quercetin-Induced PC12 Cell Death Accompanied by Caspase-Mediated DNA Fragmentation

Sasaki

Nakamura

Tsuchiya

et al. 2007

Biological & Pharmaceutical Bulletin

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“…15) Moreover, quercetin, cyanidin, and their glucosides were considered to take part in protecting against intracellular oxidative stress resulting from the radical attack. 16) We have demonstrated that the structural stabilization of the ‰avonoid glucosides was due to the introduction of the acyl moiety into their glucoside molecules. Acylation of the ‰avonoid glucosides with aromatic acids might partially decrease their radical-scavenging ability, however, the decreasing scavenging ability was not essential.…”

mentioning

confidence: 95%

Comparison of Acylated Plant Pigments: Light-resistance and Radical-scavenging Ability

Nakajima

Sugimoto

Yokoi

et al. 2003

Bioscience, Biotechnology, and Biochemistry

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“…For instance, it has been shown that quercetin conjugates are detected in rat plasma after oral administration of quercetin and that they exhibit antioxidative activities. 13,14) Recently we found that G-hesperidin is effective on hyperlipidemic subjects, suggesting that this derivative functions as hesperetin conjugates in the body. 15) Many flavonoids are hardly soluble in water.…”

mentioning

confidence: 99%

Bioavailability of Glucosyl Hesperidin in Rats

Yamada

Tanabe

Arai

et al. 2006

Bioscience, Biotechnology, and Biochemistry

128

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Effect of Quercetin and Its Conjugated Metabolite on the Hydrogen Peroxide-induced Intracellular Production of Reactive Oxygen Species in Mouse Fibroblasts

Cited by 57 publications

References 32 publications

Quercetin-Induced PC12 Cell Death Accompanied by Caspase-Mediated DNA Fragmentation

Quercetin-Induced PC12 Cell Death Accompanied by Caspase-Mediated DNA Fragmentation

Comparison of Acylated Plant Pigments: Light-resistance and Radical-scavenging Ability

Bioavailability of Glucosyl Hesperidin in Rats

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