Effect of Quercetin on Dexamethasone-Induced C2C12 Skeletal Muscle Cell Injury

Chen, Chun; Yang, Jai Sing; Lu, Chi‐Cheng; Chiu, Yu–Jen; Chen, Hung‐Che; Chung, Mei‐Ing; Wu, Yu Tse; Chen, Fu‐An

doi:10.3390/molecules25143267

Cited by 45 publications

(23 citation statements)

References 91 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Quercetin treatment prevented Dex-induced cell viability and apoptosis by regulating mitochondrial membrane potential (ΔΨm) and reducing ROS production. [ 100 ]. Moreover, in Dex-induced muscle atrophy in mice, quercetin prevented the loss of the GM by suppressing myostatin and the expression of the ubiquitin ligases atrogin-1 and MuRF-1.…”

Section: Polyphenols In Managing Muscle Atrophy and Muscle Healthmentioning

confidence: 99%

Polyphenols and Their Effects on Muscle Atrophy and Muscle Health

2021

View full text Add to dashboard Cite

Skeletal muscle atrophy is the decrease in muscle mass and strength caused by reduced protein synthesis/accelerated protein degradation. Various conditions, such as denervation, disuse, aging, chronic diseases, heart disease, obstructive lung disease, diabetes, renal failure, AIDS, sepsis, cancer, and steroidal medications, can cause muscle atrophy. Mechanistically, inflammation, oxidative stress, and mitochondrial dysfunction are among the major contributors to muscle atrophy, by modulating signaling pathways that regulate muscle homeostasis. To prevent muscle catabolism and enhance muscle anabolism, several natural and synthetic compounds have been investigated. Recently, polyphenols (i.e., natural phytochemicals) have received extensive attention regarding their effect on muscle atrophy because of their potent antioxidant and anti-inflammatory properties. Numerous in vitro and in vivo studies have reported polyphenols as strongly effective bioactive molecules that attenuate muscle atrophy and enhance muscle health. This review describes polyphenols as promising bioactive molecules that impede muscle atrophy induced by various proatrophic factors. The effects of each class/subclass of polyphenolic compounds regarding protection against the muscle disorders induced by various pathological/physiological factors are summarized in tabular form and discussed. Although considerable variations in antiatrophic potencies and mechanisms were observed among structurally diverse polyphenolic compounds, they are vital factors to be considered in muscle atrophy prevention strategies.

show abstract

Section: Polyphenols In Managing Muscle Atrophy and Muscle Healthmentioning

confidence: 99%

Polyphenols and Their Effects on Muscle Atrophy and Muscle Health

2021

View full text Add to dashboard Cite

show abstract

“…Traditional and complementary medicine has grown in popularity because of its natural appeal and availability. Thus, research has targeted safe ingredients from Chinese medicine for use as complementary medicine to reduce the side effects of the pain and anti-inflammatory drugs taken by athletes, older adults, and patients on long-term pain medication [29,89,90]. Quercetin has therapeutic potential in cases of renal damage [91,92].…”

Section: Discussionmentioning

confidence: 99%

“…Finally, 100 µL of dimethyl sulfoxide was added to the wells to dissolve formazan crystals and replace the culture medium. The optical density was measured at 570 nm by using a spectrophotometer as previously described [29,95].…”

Section: Cell Viability Assaymentioning

confidence: 99%

See 1 more Smart Citation

Effect of Quercetin on Injury to Indomethacin-Treated Human Embryonic Kidney 293 Cells

Chen

Yang

et al. 2021

Life

Self Cite

View full text Add to dashboard Cite

Nonsteroidal anti-inflammatory drugs (NSAIDs) are used to treat inflammation and pain and even to prevent the progression of cardiovascular disease. They have become widely used because of their effectiveness, especially among athletes performing high-intensity training. Indomethacin is used for pain management in sports medicine and is highly effective and versatile. However, several clinical studies have reported that indomethacin induces acute renal damage. In the present study, we determined that indomethacin reduced human embryonic kidney 293 (HEK293) cell viability in a concentration-dependent manner by triggering apoptosis. In addition, we demonstrated the effect of quercetin on indomethacin-treated HEK293 cells by inactivating the caspase-3 and caspase-9 signals. Furthermore, quercetin reduced ROS production and increased mitochondrial membrane potential (ΔΨm) in indomethacin-treated HEK293 cells. Our results indicate that quercetin can interrupt the activated caspase and mitochondrial pathway induced by indomethacin in HEK293 cells and affect apoptotic mRNA expression. Quercetin can protect against indomethacin-induced HEK293 cell apoptosis by regulating abnormal ΔΨm and apoptotic mRNA expression.

show abstract

“…Despite that quercetin supplementation inhibits muscle inflammatory cytokine TNf-α and monocyte chemoattractant protein 1(MCP-1) release following down-regulated expression of Atrogin-1/MuRf1 and prevents the obesity-induced reduction of skeletal muscle mass in high fat-fed diet C57BL/6 male mice 40 . Correspondingly quercetin supplementation increases cell viability and exerts anti-apoptotic effects on dexamethasonetreated C2C12 cells by regulating Bcl2, Bax, Apaf-1, caspase-3,9 and mitochondrial membrane potential (ΔΨm) result in attenuation in cellular oxidative stress and skeletal muscle atrophy 41 . Quercetin prevents disuse muscle atrophy in denervated mice by improving anti-oxidative capacity and decreasing hydrogen peroxide production ultimately restore protein synthesis marker pAkt/Akt and down-regulates degradation marker MuRf-1as shown in (fig.…”

Section: Quercetin Maintains Physiological Anti-oxidant and Mitochondrial Homeostasismentioning

confidence: 99%

Polyphenols as Therapeutic Approach to High Altitude Mediated Skeletal Muscle Impairments

2021

View full text Add to dashboard Cite

Skeletal muscle impairments at high altitudes resulted into various consequences in un-acclimatised individuals thus hampering their physical activities by imposing severe oxidative stress, skeletal muscle atrophy, mitochondrial dysfunction/autophagy, and regeneration disability. Researchers have described many natural and synthetic supplements to alleviate oxidative stress-induced muscle impairments. In this review article we are focusing on the skeletal muscle impairments and their alleviation by using natural polyphenols. Polyphenols are plant-based compounds showing anti-oxidative and anti-inflammatory properties like Curcumin, Catechins, Resveratrol, Quercetin and Salidrosides appear to mainly act by reversing oxidative stress and mitochondrial dysfunction eventually ameliorate skeletal muscle impairments under various imposed pathological conditions. This review also drew attention on the molecular targets of polyphenols and their possible therapeutic effects in preventing HA induced muscle impairments. Unavailability of suitable intervention, there is a need to find a probable solution having highly protective anti-atrophic, anti-oxidative, anti-inflammatory properties with the tint of performance enhancer.

show abstract

Effect of Quercetin on Dexamethasone-Induced C2C12 Skeletal Muscle Cell Injury

Cited by 45 publications

References 91 publications

Polyphenols and Their Effects on Muscle Atrophy and Muscle Health

Polyphenols and Their Effects on Muscle Atrophy and Muscle Health

Effect of Quercetin on Injury to Indomethacin-Treated Human Embryonic Kidney 293 Cells

Polyphenols as Therapeutic Approach to High Altitude Mediated Skeletal Muscle Impairments

Contact Info

Product

Resources

About