Effect of quercetin on oxidative nuclear and mitochondrial DNA damage

Abstract

Quercetin is a well-investigated antioxidant known to protect cells against oxidative nuclear DNA damage. There is no knowledge regarding its effect on oxidative mitochondrial DNA damage. In this study we investigated the effect of quercetin on oxidatively-injured DNA. Cell-free and cell studies were performed. Cell-free analyses carried out on plasmidic DNA showed that quercetin protects from all oxidative challenges used. Cellular studies were carried out on NCTC 2544 cells which were insulted with hydrogen peroxide and UVC radiations. Nuclear and mitochondrial DNAs were analysed by measuring DNA damage with a quantitative polymerase chain reaction. Quercetin supplementation showed significant genoprotective activity on mitochondrial DNA when hydroperoxide was used. The evidence of the protection afforded by quercetin suggests that this flavonoid may play an important role on mitochondrial genome stability.

Aqueous Extract from Vitis vinifera Tendrils is Able to Enrich Keratinocyte Antioxidant Defences

“…Keratinocytes NCTC 2544, incubated in DMEM, were exposed to 25, 50, 62.5 and 100 mg/mL of tendril aqueous extract for 24 h at 37°C. The membrane integrity was evaluated through the release of lactate dehydrogenase [28]. As shown in Figure 2, the keratinocytes were not damaged by the aqueous extract used in concentrations from 25 to 62.5 mg/mL.…”

Effects of Reactive Oxygen Species on Mitochondrial Content and Integrity of Human Anastomotic Colorectal Dehiscence: A Preliminary DNA Study

“…The QPCR assay had already been established with these primers in the authors' laboratory (33,34). The QPCR analyses of the two genomes showed that all three groups were not significantly different from one another, suggesting that oxidative damage in mtDNA ( Figure 3A) or in nDNA was not extensive ( Figure 3B).…”

A Multidirectional Perspective for Novel Functional Products: In vitro Pharmacological Activities and In silico Studies on Ononis natrix subsp. hispanica

“…Quercetin is a well-investigated antioxidant known as promising molecule for pharmacological studies. In a comprehensive research for DNA protection ability of quercetin, its supplementation indicated protection of nuclear DNA from oxidative damage and significant genoprotective activity on mitochondrial DNA (Potenza et al, 2008 ). In a different study, three polyphenolic compounds including luteolin, quercetin and rosmarinic acid, were investigated for their protective effects against oxidative DNA damage (Silva et al, 2008 ).…”